Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1
Yıl: 2022 Cilt: 28 Sayı: 5 Sayfa Aralığı: 643 - 652 Metin Dili: İngilizce DOI: 10.9775/kvfd.2022.27945 İndeks Tarihi: 22-10-2022
Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1
Öz: Encephalitic listeriosis is the most significant purulent encephalitis in ruminants and is a very common endemic problem in sheep, cattle, and goats. In this study, it was aimed to compare the presence of Listeria (L.) monocytogenes revealed by immunohistochemical (IHC) and Real-Time PCR methods with histopathological findings obtained from the archive materials. The study material consisted of pons and medulla oblongata paraffin tissue of 100 ruminants (9 cattle, 4 calves, 44 sheep, 38 lambs, and 5 goats). Positivity was obtained by the IHC method in 46 (46%) and by the Real-Time PCR method in 21 (21%) of 100 cases. In the L. monocytogenesis antigen IHC scoring, more severe staining was observed in sheep and goats (P>0.05). In the IHC positive cases, microabscess was more severe in sheep and goats than in cattle and lambs (P<0.05). In addition, 19 patients had Coenurus cerebralis cysts, and 3 of them were found to be positive for the IHC agent of Listeria. It was concluded that IHC and PCR methods can be used to detect L. monocytogenes from paraffin blocks, but the IHC method is a more effective method than PCR in revealing the presence of antigen from paraffin blocks stored for many years.
Anahtar Kelime: Ensefalitisli Ruminantlarda Listeriyozisin Tanısı İçin Histopatolojik, İmmünohistokimyasal ve Real-Time PCR Yöntemlerinin Karşılaştırılması
Öz: Ensefalitik listeriozis, ruminantlardaki purulent ensefalitisler arasında en önemlisidir ve tüm dünyada koyun, sığır ve keçilerde oldukça yaygın görülen endemik bir problemdir. Bu çalışmada, immünohistokimyasal (IHC) ve Real-Time PCR yöntemleri ile ortaya konan Listeria (L.) –monocytogenes’in varlığının arşiv materyallerinden elde edilen histopatolojik bulgularla karşılaştırılması amaçlandı. Çalışma materyalini 100 ruminantın (9 sığır, 4 buzağı, 44 koyun, 38 kuzu ve 5 keçi) pons ve medulla oblongata parafin dokuları oluşturdu. İncelenen 100 olgunun 46’sında (%46) IHC yöntemi ile, 21’inde ise (%21) Real-Time PCR yöntemi ile pozitiflik elde edildi. IHC skorlamasında L. monocytogenes antijeni koyun ve keçilerde daha belirgin boyandığı gözlendi (P>0.05). IHC pozitif vakalarda, koyun ve keçilerde, sığır ve kuzulara göre mikroapse daha şiddetliydi (P<0.05). Ayrıca 19 hastada Coenurus cerebralis kisti vardı ve bunlardan 3’ünde IHC yöntemiyle Listeria etkeni pozitif bulundu. IHC ve PCR yöntemlerinin parafin bloklarından L. monocytogenes tespitinde kullanılabileceği, ancak IHC yönteminin uzun yıllar saklanan parafin bloklarından antijen varlığını ortaya çıkarmada PCR’den daha etkili bir yöntem olduğu sonucuna varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Hatipoglu F, Terzi F, Ozdemir O, ORTATATLI M, ÇIFTÇI M, ATEŞ M (2022). Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. , 643 - 652. 10.9775/kvfd.2022.27945 |
| Chicago | Hatipoglu Fatih,Terzi Funda,Ozdemir Ozgur,ORTATATLI MUSTAFA,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. (2022): 643 - 652. 10.9775/kvfd.2022.27945 |
| MLA | Hatipoglu Fatih,Terzi Funda,Ozdemir Ozgur,ORTATATLI MUSTAFA,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. , 2022, ss.643 - 652. 10.9775/kvfd.2022.27945 |
| AMA | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. . 2022; 643 - 652. 10.9775/kvfd.2022.27945 |
| Vancouver | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. . 2022; 643 - 652. 10.9775/kvfd.2022.27945 |
| IEEE | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M "Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1." , ss.643 - 652, 2022. 10.9775/kvfd.2022.27945 |
| ISNAD | Hatipoglu, Fatih vd. "Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1". (2022), 643-652. https://doi.org/10.9775/kvfd.2022.27945 |
| APA | Hatipoglu F, Terzi F, Ozdemir O, ORTATATLI M, ÇIFTÇI M, ATEŞ M (2022). Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(5), 643 - 652. 10.9775/kvfd.2022.27945 |
| Chicago | Hatipoglu Fatih,Terzi Funda,Ozdemir Ozgur,ORTATATLI MUSTAFA,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.5 (2022): 643 - 652. 10.9775/kvfd.2022.27945 |
| MLA | Hatipoglu Fatih,Terzi Funda,Ozdemir Ozgur,ORTATATLI MUSTAFA,ÇIFTÇI MUSTAFA KEMAL,ATEŞ Mehmet Burak Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.5, 2022, ss.643 - 652. 10.9775/kvfd.2022.27945 |
| AMA | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(5): 643 - 652. 10.9775/kvfd.2022.27945 |
| Vancouver | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(5): 643 - 652. 10.9775/kvfd.2022.27945 |
| IEEE | Hatipoglu F,Terzi F,Ozdemir O,ORTATATLI M,ÇIFTÇI M,ATEŞ M "Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.643 - 652, 2022. 10.9775/kvfd.2022.27945 |
| ISNAD | Hatipoglu, Fatih vd. "Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis [1". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/5 (2022), 643-652. https://doi.org/10.9775/kvfd.2022.27945 |
