Yıl: 2022 Cilt: 9 Sayı: 4 Sayfa Aralığı: 538 - 550 Metin Dili: İngilizce DOI: 10.21448/ijsm.1084743 İndeks Tarihi: 23-12-2022

The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia

Öz:
The present study focused on chemical composition and base therapeutic potential of common three Scorzonera species (Scorzonera mollis M.Bieb. subsp. mollis, Scorzonera papposa DC. and Scorzonera semicana DC), which have been utilized as food and medicine by local people of Eastern Anatolia for a long time. Comparative analytical studies were performed on ethanol-based extracts and traditional preparations (infusions) through chromatographic (HPLC-MS/MS) and reagent-based antioxidant and enzyme inhibitory assays. Results revealed that leaf extracts were rich in phenolics, particularly hydroxycinnamic acids that were confirmed by HPLC-MS/MS, chlorogenic acids and luteolin were the major phytochemical compounds of extracts. With regards to biological activities findings, it was determined that ethanol-based extracts showed better antioxidant activities and effectively suppressed the activities of α-glucosidase. In addition, both of the extracts were found as strong suppressive agents of pancreatic lipase activity so Scorzonera species were rich sources of bioactive compounds that able to deactivate reactive oxygen species and free radicals and as well as suppress the activities of α-glucosidase and pancreatic lipase. Finally, obtained findings reveal base data of Scorzonera species for researches that focused on novel candidates of nutraceuticals and biotherapeutics.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Abd El Raheim, M.D. (2016). Phytochemical and pharmacological studies on Scorzonera alexandrina Boiss. Journal of Saudi Chemical Society, 20, S433 S439. http://dx.doi.org/10.1016/j.jscs.2013.01.001
  • Agarwal, P., & Gupta, R. (2016). Alpha-amylase inhibition can treat diabetes mellitus. Research & Reviews: Journal of Medical and Health Sciences, 5(4), 1-8.
  • Ainsworth, E.A., & Gillespie, K.M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nature Protocols, 2(4), 875-877. https://doi.org/10.1038/nprot.2007.102.
  • Ak, G., Dall'Acqua, S., Sut, S., Ferrarese, I., Yıldıztugay, E., Mahomoodally, M.F., & Zengin, G. (2020). Chemical characterization and bio-pharmaceutical abilities of five different solvent extracts from aerial parts and roots of Scorzonera hispanica L. South African Journal of Botany, 133(1), 212-221. https://doi.org/10.1016/j.sajb.2020.08.003
  • Baytop, T. (1999). Therapy with Medicinal Plants in Turkey (Past and Present). Publication of the Istanbul University, 312, Türkiye.
  • Benzie, I.F.F., & Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical Biochemistry, 23(1)9, 706. https://doi.org/10.1006/abio.1996.0292.
  • Boussaada, O., Saidana, D., Chriaa, J., Chraif, I., Ammar Mahjoub, M.A., & Helal, A.N. (2008). Chemical composition and antimicrobial activity of volatile components of Scorzonera undulata. Journal of Essential Oil Research, 20(4), 358 362. https://doi.org/10.1080/10412905.2008.9700030
  • Dalar, A., & Konczak, I. (2013). Phenolic contents, antioxidant capacities and inhibitory activities against key metabolic syndrome relevant enzymes of herbal teas from Eastern Anatolia. Industrial Crops and Products, 44(1), 383 390. https://doi.org/10.1016/j.indcrop.2012.11.037
  • Dalar, A., Türker, M., & Konczak, I. (2012). Antioxidant capacity and phenolic constituents of Malva neglecta Wallr. and Plantago lanceolata L. from Eastern Anatolia Region of Turkey. Journal of Herbal Medicine, 2(2), 42 51. https://doi.org/10.1016/j.hermed.2012.03.001
  • Dall'Acqua, S., Ak, G., Sut, S., Zengin, G., Yıldıztugay, E., Mahomoodally, M.F., & Lobine, D. (2020). Comprehensive bioactivity and chemical characterization of the endemic plant Scorzonera hieraciifolia Hayek extracts: A promising source of bioactive compounds. International Food Research Journal, 137(1), 109371. https://doi.org/10.1016/j.foodres.2020.109371
  • Ergün, F. (2021). Cotoneaster transcaucasicus Pojark. Determination of bioactive component amounts and antioxidant activities in fruit extracts. Food Science and Technology, 9(7), 1258-1263.
  • Ergün, F. (2022). The Effects of Drying Methods on total phenolic and flavonoid substances and antioxidant capacity of redstem filaree Erodium cicutarium. Applied Ecology and Environmental Research, 20(1), 499-509.
  • Funk, V.A, Anderberg, A.A., Baldwin, B.G., Bayer, R.J., Bonifacino, J.M., Breitwieser, I., & Crawford, D.J. (2009). Compositae Metatrees: The next generation. systematics, evolution, and biogeography of Compositae. Institute of Botany, University of Vienna, Rennweg/Austria.
  • Gironés-Vilaplana, A., Valentão, P., Andrade, P.B., Ferreres, F., Moreno, D.A., & García-Viguera, C. (2012). Phytochemical profile of a blend of black chokeberry and lemon juice with cholinesterase inhibitory effect and antioxidant potential. Food Chemistry, 134(4), 2090-2096. https://doi.org/10.1016/j.foodchem.2012.04.010
  • Goncalves, S., & Romano, A. (2017). Inhibitory properties of phenolic compounds against enzymes linked with human diseases. Phenolic compounds-biological activity. London: IntechOpen, 99-118.
  • Granica, S., & Zidorn, C. (2015). Phenolic compounds from aerial parts as chemosystematic markers in the Scorzonerinae (Asteraceae). Biochemical Systematics and Ecology, 58(1), 102-113. https://doi.org/10.1016/j.bse.2014.11.005
  • Gülçin., İ., Huyut, Z., Elmastaş, M., & Aboul-Enein, H.Y. (2010). Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry, 3(1), 43-53. https://doi.org/10.1016/j.arabjc.2009.12.008
  • Hattori, E.K., & Nakajima, J.N. (2008). A família Asteraceae na estação de pesquisa e desenvolvimento ambiental galheiro, perdizes, Minas Gerais, Brasil. Rodriguésia. 59(4), 687-749. https://doi.org/10.1590/2175-7860200859405
  • Jiang, T.F., Wang, Y.H., Lv Z.H., & Yue, M.E. (2007). Determination of kava lactones and flavonoid glycoside in Scorzonera austriaca by capillary zone electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 43(3), 854 858. https://doi.org/10.1016/j.jpba.2006.08.024
  • Kenny, O., Smyth, T.J., Walsh, D., Kelleher, C.T., Hewage, C.M, & Brunton N.P. (2014). Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts. Food Chemistry, 161(1), 79-86. https://doi.org/10.1016/j.foodchem.2014.03.126
  • Konczak, I., Yoshimoto, M., Hou, D.X., Terahara, N., & Yamakawa, O. (2003). Potential chemopreventive properties of anthocyanin-rich aqueous extracts from in vitro produced tissue of Sweetpotato (Ipomoea batatas L.). Journal of Agricultural and Food Chemistry, 51(1), 5916-5922. https://doi.org/10.1021/jf030066o
  • Kwon, Y.I, Apostolidis, E., & Shetty, K. (2008). Inhibitory potential of wine and tea against α Amylase and α Glucosidase for management of hyperglycemia linked to type 2 diabetes. Journal of Food Biochemistry, 32(1), 15-31. https://doi.org/10.1111/j.1745-4514.2007.00165.x
  • Luthria, D.L., Biswas, R., & Natarajan, S. (2007). Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean. Food Chemistry, 105(1), 325-333. https://doi.org/10.1016/j.foodchem.2006.11.047
  • Mai, T.T., Thu, N.N., Tien, P.G., & Van Chuyen, N. (2007). Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. Journal of Nutritional Science and Vitaminology, 53(3), 267-276. https://doi.org/10.3177/jnsv.53.267
  • Martinez-Gonzalez, A.I., Alvarez-Parrilla, E., Díaz-Sánchez, Á.G., de la Rosa, L.A., Núñez-Gastélum, J.A., Vazquez-Flores, A.A., & Gonzalez-Aguilar, G.A. (2017). In vitro inhibition of pancreatic lipase by polyphenols: A kinetic, fluorescence spectroscopy and molecular docking study. Food Technology and Biotechnology, 55(4), 519 530. https://doi.org/10.17113/ftb.55.04.17.5138
  • Milella, L., Bader, A., De Tommasi, N., Russo. D., & Braca, A. (2014). Antioxidant and free radical-scavenging activity of constituents from two Scorzonera species. Food Chemistry, 160, 298-304. https://doi.org/10.1016/j.foodchem.2014.03.097
  • Moe, T.S., Win, H.H., Hlaing, T.T., Lwin, W.W., Htet, Z.M., & Mya, K.M. (2018). Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants. Journal of Integrative Medicine, 16(5), 358 366. https://doi.org/10.1016/j.joim.2018.08.001
  • Mukemre, M., Kończak, I., Uzun, Y., & Dalar A. (2020). Phytochemical profile and biological activities of Anatolian plantain (Plantago anatolica). Food Bioscience, 36(1), 100658. https://doi.org/10.1016/j.fbio.2020.100658
  • Oboh, G., Agunloye, O.M., Adefegha, S.A., Akinyemi, A.J., & Ademiluyi, A.O. (2015). Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study. Journal of Basic and Clinical Physiology and Pharmacology, 26(2), 165-170. https://doi.org/10.1515/jbcpp-2013-0141
  • Özgökçe, F., & Özçelik, H. (2004). Ethnobotanical aspects of some taxa in East Anatolia, Turkey. Economic Botany, 58(4), 697-704.
  • Petrovska, B.B. (2012). Historical Review of Medicinal Plants’ Usage. Pharmacognosy Reviews, 6(11), 1-5. https://doi.org/0.4103/0973-7847.95849
  • Pinto, M.S., Lajolo, F.M., & Genoves, M.I.G. (2008). Bioactive compounds and quantification of total ellagic acid in strawberries (Fragaria x ananassa Duch.). Food Chemistry, 107(1), 1629-35. https://doi.org/10.1016/j.foodchem.2007.10.038
  • Prior, R.L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53, 4290–302. https://doi.org/10.1021/jf0502698
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  • Rees, S., & Harborne, J. (1984). Flavonoids and other phenolics of Cichorium and related members of the Lactuceae (Compositae). Botanical Journal of the Linnean Society, 89(4), 313-319. https://doi.org/10.1111/j.1095-8339.1984.tb02563.x
  • Reid, A.M., Oosthuizen, C.B., Fibrich, B.D., Twilley, D., Lambrechts, I.A, de Canha, M.N., & Lall, N. (2018). Traditional Medicine: the Ancient Roots of Modern Practice. In medicinal plants for holistic health and well-Being. Academic Press. 1-11.
  • Şahin, H., Sarı, A., Özsoy, N., Özbek, Ç.B., & Koyuncu, O. (2020). Two new phenolic compounds and some biological activities of Scorzonera pygmaea Sibth. & Sm. subaerial parts. Natural Product Research, 34(5), 621 628. https://doi.org/10.1080/14786419.2018.1493585
  • Sarı, A., Şahin, H., Özsoy, N., & Çelik, B.Ö. (2019). Phenolic compounds and in vitro antioxidant, anti-inflammatory, antimicrobial activities of Scorzonera hieraciifolia Hayek roots. South African Journal of Botany. 125(1), 116 119. https://doi.org/10.1016/j.sajb.2019.07.009
  • Sakulnarmrat, K., & Konczak, I., (2012). Composition of native Australian herbs polyphenolic-rich fractions and in vitro inhibitory activities against key enzymes relevant to metabolic syndrome. Food Chemistry, 134(2), 1011 1019. https://doi.org/10.1016/j.foodchem.2012.02.217
  • Schütz, K., Kammerer, D.R., Carle, R., & Schieber, A. (2005). Characterisation of phenolic acids and flavonoids in dandelion (Taraxacum officinale WEB. ex WIGG) root and herb by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 19(1), 179 186. https://doi.org/10.1002/rcm.1767
  • Slanc, P., Doljak, B., Kreft, S., Lunder, M., Janeš, D., & Štrukelj, B. (2009). Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition. Phytotherapy Research, 23(6), 874-877. https://doi.org/10.1002/ptr.2718
  • Souza, S.P.D., Pereira, L.L., Souza, A.A, & Santos, C.D.D. (2011). Inhibition of pancreatic lipase by extracts of Baccharis trimera (Less.) DC., Asteraceae: evaluation of antinutrients and effect on glycosidases. Revista Brasileira de Farmacognosia, 21(3), 450-455. https://doi.org/10.1590/S0102-695X2011005000049
  • Taskin, D., Gecim, M., Dogan, A., & Beceren, A. (2021). Polyphenolic composition and Antioxidant Effect of Aerial Parts and Roots Extracts from Scorzonera veratrifolia. International Journal of Secondary Metabolite, 8 (3), 284–299
  • Vergun, O., Kačániová, M., Rakhmetov, D., Shymanska, O., Bondarchuk, O., Brindza, J., & Ivanišová, E. (2018). Antioxidant and antimicrobial activity of Bunias orientalis L. and Scorzonera hispanica L. ethanol extracts Agrobiodiversity for Improving Nutrition, Health and Life Quality, 5(2), 29-38.
  • Wongsa, P., Chaiwarit, J., & Zamaludien, A. (2012). In vitro screening of phenolic compounds, potential inhibition against α-amylase and α-glucosidase of culinary herbs in Thailand. Food Chemistry, 131(3), 964-971. https://doi.org/10.1016/j.foodchem.2011.09.088
APA hasan s, EREZ M, Mukemre M, Dalar A (2022). The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. , 538 - 550. 10.21448/ijsm.1084743
Chicago hasan sazgar,EREZ Mehmet Emre,Mukemre Muzaffer,Dalar Abdullah The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. (2022): 538 - 550. 10.21448/ijsm.1084743
MLA hasan sazgar,EREZ Mehmet Emre,Mukemre Muzaffer,Dalar Abdullah The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. , 2022, ss.538 - 550. 10.21448/ijsm.1084743
AMA hasan s,EREZ M,Mukemre M,Dalar A The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. . 2022; 538 - 550. 10.21448/ijsm.1084743
Vancouver hasan s,EREZ M,Mukemre M,Dalar A The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. . 2022; 538 - 550. 10.21448/ijsm.1084743
IEEE hasan s,EREZ M,Mukemre M,Dalar A "The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia." , ss.538 - 550, 2022. 10.21448/ijsm.1084743
ISNAD hasan, sazgar vd. "The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia". (2022), 538-550. https://doi.org/10.21448/ijsm.1084743
APA hasan s, EREZ M, Mukemre M, Dalar A (2022). The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. International Journal of Secondary Metabolite, 9(4), 538 - 550. 10.21448/ijsm.1084743
Chicago hasan sazgar,EREZ Mehmet Emre,Mukemre Muzaffer,Dalar Abdullah The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. International Journal of Secondary Metabolite 9, no.4 (2022): 538 - 550. 10.21448/ijsm.1084743
MLA hasan sazgar,EREZ Mehmet Emre,Mukemre Muzaffer,Dalar Abdullah The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. International Journal of Secondary Metabolite, vol.9, no.4, 2022, ss.538 - 550. 10.21448/ijsm.1084743
AMA hasan s,EREZ M,Mukemre M,Dalar A The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. International Journal of Secondary Metabolite. 2022; 9(4): 538 - 550. 10.21448/ijsm.1084743
Vancouver hasan s,EREZ M,Mukemre M,Dalar A The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia. International Journal of Secondary Metabolite. 2022; 9(4): 538 - 550. 10.21448/ijsm.1084743
IEEE hasan s,EREZ M,Mukemre M,Dalar A "The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia." International Journal of Secondary Metabolite, 9, ss.538 - 550, 2022. 10.21448/ijsm.1084743
ISNAD hasan, sazgar vd. "The phenolic profile and biological activities of common Scorzonera species from Eastern Anatolia". International Journal of Secondary Metabolite 9/4 (2022), 538-550. https://doi.org/10.21448/ijsm.1084743