Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease

altuğ, nuri; ÖZKAN, CUMALİ; Yuksek, Nazmi; kozat, Süleyman

Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease

Süleyman KOZAT, (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Van, Türkiye)

Nuri ALTUĞ, (Mustafa Kemal Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Hatay, Türkiye)

Nazmi YÜKSEK, (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Van, Türkiye)

Cumali ÖZKAN (Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Van, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

4 1

Yıl: 2011 Cilt: 17 Sayı: 3 Sayfa Aralığı: 441 - 444 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease

Öz:

Beyaz kas hastalığı veya besinsel kas dejenerasyonları, hayvanlarda iskelet ve kalp kaslarının dejeneratif değişiklikleri ile karakterize bir hastalığıdır. Bu çalışmada kuzulardaki subklinikal beyaz kas hastalığında plazma homosistein (Hcy), serum kardiak troponin I (cTnI) ve nitrik oksit (NO) düzeyleri araştırıldı. Çalışmada 10 sağlıklı ve 20 subklinikal beyaz kas hastalıklı kuzu kullanıldı. Kan örnekleri NMD kuzularda tedavi öncesi, tedavi sonrası 3. ve 10. günlerde ve kontrol grubunda bir kez alındı. Serum kreatinin kinaz (CK), aspartat aminotransferaz (AST), alanin aminotranferaz (ALT), alkalin fosfataz (ALP) ve laktat dehidrogenaz (LDH) değerleri otoanalizatör cihazıyla ölçüldü. Plazma homosistein konsantrasyonu, serum cTn I ve NO düzeyleri ELİSA cihazıyla ve eritrosit glutathione peroxidase (GSH-Px) aktivitesi ise spektrofotometre cihazıyla ölçüldü. Tedavi öncesi, kuzuların subklinik beyaz kas hastalığında GSH-Px aktiviteleri sağlıklı kuzulara göre daha düşük iken (P<0.001), plazma Hcy, serum NO, cTn I, AST, ALT, ALP, CK ve LDH değerleri sağlıklı kuzuların değerlerinden yüksek bulundu (P<0.001). Tedavi sonrası 10. günde elde edilen ortalama değerler, kontrol grubunun değerlerinden farklı değildi. Kuzuların subklinik beyaz kas hastalığında Hcy, cTn I ve NO değerlerindeki yükselmelerin, selenyum eksikliğinin sebep olduğu miyokardiyal hasarlarına bağlı gelişebileceği düşünüldü.

Anahtar Kelime: kan kimyası aspartat aminotransferaz hayvan patolojisi alanin aminotransferaz troponin homosistein laktat dehidrogenaz alkalin fosfotaz kuzu kreatin kinaz enzim aktivitesi nitrik oksit kas distrofisi glutatyon peroksidaz

Konular: Veterinerlik

Subklinik beyaz kas hastalıklı kuzularda homosistein, troponin I ve nitrik oksit düzeylerinin değerlendirilmesi

Öz:

White muscle disease (WMD) or Nutritional myodegeneration disease (NMD) is a degenerative disease of the cardiac and skeletal muscles. The level of serum activity of Plasma homocysteine (Hcy), cardiac troponin I (cTn I), Nitric oxide (NO) were investigated in lambs with subclinical NMD in this study. Ten healthy lambs and twenty lambs with subclinical NMD were used in this study. The blood samples were firstly taken at the onset of the disease and then 3 and 10 days after treatment in NMD, and taken once control group. The values of serum activity of creatin kinase (CK), aspartate aminotranferase (AST), alanine aminotranferase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) were measured with autoanalyzer. Plasma concentration of Hcy, serum cTn I, and levels of NO were determined with ELISA method and value of activity erythrocyte glutathione peroxidase (GSH-Px) was measured with spectrophotometer. Before treatment, values of plasma Hcy, serum NO, cTn I, AST, ALT, ALP, CK, and LDH in lambs with NMD were higher than those of healthy ones (P<0.001), while GSH-Px activity of lambs with NMD are lower than those of healthy (P<0.001). After ten days of treatment, there were no significant diff erences between treated lambs with NMD and healthy lambs. Nutritional myodegeneration in lambs is associated with increased levels of Hcy, cTn I, and NO. Increased levels of Hcy, cTn I, and NO may be a result of selenium deficiency causing myocardial disorder in NMD lambs.

Anahtar Kelime: lambs creatine kinase enzyme activity nitric oxide muscular dystrophy glutathione peroxidase blood chemistry aspartate aminotransferase animal pathology alanine aminotransferase troponins homocysteine lactate dehydrogenase alkaline phosphatase

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	kozat S, altuğ n, Yuksek N, ÖZKAN C (2011). Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. , 441 - 444.
Chicago	kozat Süleyman,altuğ nuri,Yuksek Nazmi,ÖZKAN CUMALİ Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. (2011): 441 - 444.
MLA	kozat Süleyman,altuğ nuri,Yuksek Nazmi,ÖZKAN CUMALİ Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. , 2011, ss.441 - 444.
AMA	kozat S,altuğ n,Yuksek N,ÖZKAN C Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. . 2011; 441 - 444.
Vancouver	kozat S,altuğ n,Yuksek N,ÖZKAN C Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. . 2011; 441 - 444.
IEEE	kozat S,altuğ n,Yuksek N,ÖZKAN C "Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease." , ss.441 - 444, 2011.
ISNAD	kozat, Süleyman vd. "Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease". (2011), 441-444.

APA	kozat S, altuğ n, Yuksek N, ÖZKAN C (2011). Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(3), 441 - 444.
Chicago	kozat Süleyman,altuğ nuri,Yuksek Nazmi,ÖZKAN CUMALİ Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, no.3 (2011): 441 - 444.
MLA	kozat Süleyman,altuğ nuri,Yuksek Nazmi,ÖZKAN CUMALİ Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.3, 2011, ss.441 - 444.
AMA	kozat S,altuğ n,Yuksek N,ÖZKAN C Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(3): 441 - 444.
Vancouver	kozat S,altuğ n,Yuksek N,ÖZKAN C Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(3): 441 - 444.
IEEE	kozat S,altuğ n,Yuksek N,ÖZKAN C "Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.441 - 444, 2011.
ISNAD	kozat, Süleyman vd. "Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17/3 (2011), 441-444.