Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization

Yildirim, Tuba; YURDUSEV, Nevzat; BÜYÜKTANIR YAŞ, ÖZLEM; FINDIK, ARZU; Yağcı, Tamer

Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization

ÖZLEM BÜYÜKTANIR YAŞ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Kontrol Bölümü, Samsun,Türkiye)

Tamer YAĞCI, (Bilkent Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Ankara, Türkiye)

Arzu FINDIK, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Kontrol Bölümü, Samsun,Türkiye)

Tuba YILDIRIM, (Amasya Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Amasya, Türkiye)

Nevzat YURDUSEV (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Kontrol Bölümü, Samsun,Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2011 Cilt: 17 Sayı: 4 Sayfa Aralığı: 667 - 670 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization

Öz:

Salmonella Enteritidis’e karşı monoklonal antikor üretmek amacıyla BALB/c fareler inaktif bakteri ile periton içi bağışıklandı. Beşincibağışıklamada en yüksek yanıtı veren hayvana ait dalak hücreleri fare Sp2/0 miyeloma hücrelerinin füzyon partneri olarak kullanıldı.IgM ve IgG izotipi antikorlar salgılayan toplam 6 adet kararlı hibridoma hücresi elde edildi. Bu hibridomaların Western blot analizinde S.Enteritidis’e ait 73, 59 ve 42 kDa relatif molekül ağırlıklarına sahip 3 antijen ile reaksiyon verdiği belirlendi. 59 kDa molekül Salmonellaflagellin proteinine denk düşmektedir. Bu ön çalışmada, monoepitopik ve monoizotipik antikor salgılayan monoklonal hibrid hücrelerinelde edilmesi için ilave araştırmaların yapılmasının gerekli olduğu sonucuna varılmıştır.

Anahtar Kelime: fareler Salmonella enteritidis dalak hücreleri inaktivasyon antikor hibridoma

Konular: Veterinerlik

Anti-Salmonella Enteritidis antikorları salgılayan fare hibridomalarının geliştirilmesi ve ön karakterizasyonları

Öz:

BALB/c mice were intraperitoneally immunized with inactivated bacteria for generation of monoclonal anti-S. Enteritidis antibody.The spleen cells of the highest responder animal at fifth immunization were used as the fusion partner of the mouse Sp2/0 myelomacells. A total of 6 stable hybridomas secreting IgM and IgG isotype antibodies were obtained. These hybridomas were found to bereactive with three S. Enteritidis antigens having relative molecular weights of 73, 59 and 42kDa in Western blot analysis. The 59kDamolecule corresponds to the flagellin protein. From this preliminary study, it can be concluded that further investigations are necessaryto obtain monoclonal hybrid cells secreting monoepitopic and monoisotypic antibody by subcloning of the parental hybridomas.

Anahtar Kelime: inactivation antibodies hybridomas mice Salmonella enteritidis spleen cells

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	BÜYÜKTANIR YAŞ Ö, Yağcı T, FINDIK A, Yildirim T, YURDUSEV N (2011). Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. , 667 - 670.
Chicago	BÜYÜKTANIR YAŞ ÖZLEM,Yağcı Tamer,FINDIK ARZU,Yildirim Tuba,YURDUSEV Nevzat Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. (2011): 667 - 670.
MLA	BÜYÜKTANIR YAŞ ÖZLEM,Yağcı Tamer,FINDIK ARZU,Yildirim Tuba,YURDUSEV Nevzat Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. , 2011, ss.667 - 670.
AMA	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. . 2011; 667 - 670.
Vancouver	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. . 2011; 667 - 670.
IEEE	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N "Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization." , ss.667 - 670, 2011.
ISNAD	BÜYÜKTANIR YAŞ, ÖZLEM vd. "Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization". (2011), 667-670.

APA	BÜYÜKTANIR YAŞ Ö, Yağcı T, FINDIK A, Yildirim T, YURDUSEV N (2011). Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(4), 667 - 670.
Chicago	BÜYÜKTANIR YAŞ ÖZLEM,Yağcı Tamer,FINDIK ARZU,Yildirim Tuba,YURDUSEV Nevzat Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, no.4 (2011): 667 - 670.
MLA	BÜYÜKTANIR YAŞ ÖZLEM,Yağcı Tamer,FINDIK ARZU,Yildirim Tuba,YURDUSEV Nevzat Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.4, 2011, ss.667 - 670.
AMA	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(4): 667 - 670.
Vancouver	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(4): 667 - 670.
IEEE	BÜYÜKTANIR YAŞ Ö,Yağcı T,FINDIK A,Yildirim T,YURDUSEV N "Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.667 - 670, 2011.
ISNAD	BÜYÜKTANIR YAŞ, ÖZLEM vd. "Generation of mouse hybridomas secreting anti-Salmonella Enteritidis antibodies and their preliminary characterization". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17/4 (2011), 667-670.