Yıl: 2023 Cilt: 9 Sayı: 2 Sayfa Aralığı: 136 - 141 Metin Dili: İngilizce DOI: 10.53394/akd.1027038 İndeks Tarihi: 22-05-2023

Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Öz:
Objective: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion (RIR) method. Material and Method: In the existing study, experimental animals were randomlyassigned to 4 groups as sham, RIR, Dimethyl Sulfoxide (DMSO) and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion (IR) model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0.3 ml, 1% DMSO was administered intraperitoneally. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0.5 mg/ml for each rat every other days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated. Tumor Necrosis Factor alpha (TNF-α), Malondialdehyde (MDA), Interleukin 1beta (IL-1 β), Oxidative Stress Index (OSI), Total Oxidant Status (TOS), Total Antioxidant Status (TAS) levels, Superoxide Dismutase (SOD) and Myeloperoxidase (MPO) activity in tissue samples were analyzed using biochemical methods. Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatol+I/R group due to brusatol therapy compared to sham and DMSO groups. Conclusion: It has been thought that brusatol alleviated oxidative damage thanks to its antioxidant and anti-inflammatory roperties. Consequently, brusatol demonstrated protective effects against RIR-induced oxidative kidney injury in rats.
Anahtar Kelime: Brusatol Renal Ischemia Reperfusion Oxidative stress Rat

Brusatol'ün Renal İskemi Reperfüzyon Hasarına Karşı Renoprotektif Etkileri

Öz:
Amaç: Bu çalışmanın amacı, brusatol tedavisinin bilateral renal iskemi reperfüzyon (RIR) yöntemi ile indüklenen oksidatif böbrek hasarı üzerindeki potansiyel yararlı etkilerini belirlemektir. Gereç ve Yöntem: Mevcut çalışmada, deney hayvanları rastgele sham, renal iskemi reperfüzyonu (RIR), Dimetil sülfoksit (DMSO) ve brusatol grupları olarak 4 gruba ayrıldı. Sham grubu; arka bölge insizyon ile açıldı ve sonra sütüre edildi ancak iskemi reperfüzyon (IR) modeli oluşturulmadı. RIR grubunda 1 saatlik iskemiyi takiben 24 saatlik reperfüzyon oluşturuldu. DMSO grubunda, her sıçan için 0,3 ml %1 DMSO intraperitonal olarak uygulandı. Daha sonra RIR grubunda anlatıldığı gibi IR modeli uygulandı. Brusatol grubunda brusatol, deneyden önce 10 gün boyunca her gün her bir sıçan için 0,5 mg/ml olarak intraperitonal olarak uygulandı. Son doz reperfüzyondan 30 dakika önce uygulandı ve IR, RIR grubunda gösterildiği gibi gerçekleştirildi. Reperfüzyon döneminden sonra sıçanlar hareketsiz hale getirildi ve böbrek dokuları izole edildi. Doku örneklerinde Tümör Nekroz Faktör Alfa (TNF-α), Malondialdehit (MDA), İnterlökin 1Beta (IL-1β), Oksidatif Stres İndeksi (OSI), Total Oksidan Seviyesi (TOS), Total Antioksidan Seviyesi (TAS) düzeyleri, SOD ve MPO aktivitesi biyokimyal metodlar kullanılarak analiz edildi. Bulgular: TNF-α, MDA ve IL-1β düzeyleri, OSI, TOS ve MPO değerleri anlamlı olarak yükselmiş, ancak TAS ve SOD düzeyleri sham grubuna göre RIR ve DMSO gruplarında azalmıştır. Öte yandan TAS ve SOD artarken OSI ve TOS değerleri, MPO ve TNF-α, MDA ve IL-1β düzeylerinin brusatol+I/R grubunda sham ve DMSO gruplarına göre brusatol tedavisine bağlı olarak anlamlı düzeyde azaldığı görülmüştür. Sonuç: Brusatolün sahip olduğu antioksidan ve anti-enflamatuar özellikleri sayesinde oksidatif hasarı hafifletmiş olduğu düşünüldü. Sonuç olarak, brusatolun sıçanlarda RIR kaynaklı oksidatif böbrek hasarına karşı koruyucu etkiler gösterdiği söylenebilir.
Anahtar Kelime: Brusatol Renal İskemi Reperfüzyonu Oksidatif stres Sıçan

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Ekinci Akdemir F, tanyeli a, Eraslan E (2023). Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. , 136 - 141. 10.53394/akd.1027038
Chicago Ekinci Akdemir Fazile Nur,tanyeli ayhan,Eraslan Ersen Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. (2023): 136 - 141. 10.53394/akd.1027038
MLA Ekinci Akdemir Fazile Nur,tanyeli ayhan,Eraslan Ersen Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. , 2023, ss.136 - 141. 10.53394/akd.1027038
AMA Ekinci Akdemir F,tanyeli a,Eraslan E Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. . 2023; 136 - 141. 10.53394/akd.1027038
Vancouver Ekinci Akdemir F,tanyeli a,Eraslan E Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. . 2023; 136 - 141. 10.53394/akd.1027038
IEEE Ekinci Akdemir F,tanyeli a,Eraslan E "Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury." , ss.136 - 141, 2023. 10.53394/akd.1027038
ISNAD Ekinci Akdemir, Fazile Nur vd. "Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury". (2023), 136-141. https://doi.org/10.53394/akd.1027038
APA Ekinci Akdemir F, tanyeli a, Eraslan E (2023). Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. Akdeniz Tıp Dergisi, 9(2), 136 - 141. 10.53394/akd.1027038
Chicago Ekinci Akdemir Fazile Nur,tanyeli ayhan,Eraslan Ersen Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. Akdeniz Tıp Dergisi 9, no.2 (2023): 136 - 141. 10.53394/akd.1027038
MLA Ekinci Akdemir Fazile Nur,tanyeli ayhan,Eraslan Ersen Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. Akdeniz Tıp Dergisi, vol.9, no.2, 2023, ss.136 - 141. 10.53394/akd.1027038
AMA Ekinci Akdemir F,tanyeli a,Eraslan E Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. Akdeniz Tıp Dergisi. 2023; 9(2): 136 - 141. 10.53394/akd.1027038
Vancouver Ekinci Akdemir F,tanyeli a,Eraslan E Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury. Akdeniz Tıp Dergisi. 2023; 9(2): 136 - 141. 10.53394/akd.1027038
IEEE Ekinci Akdemir F,tanyeli a,Eraslan E "Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury." Akdeniz Tıp Dergisi, 9, ss.136 - 141, 2023. 10.53394/akd.1027038
ISNAD Ekinci Akdemir, Fazile Nur vd. "Reno-Protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury". Akdeniz Tıp Dergisi 9/2 (2023), 136-141. https://doi.org/10.53394/akd.1027038