Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes

TÜYLÜ, Aslı özkök; TEMİZ, Ayhan; ŞENER, Ayla; SORKUN, KADRİYE; Salih, Bekir

Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes

Ayhan TEMİZ, (Hacettepe Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, Ankara, Türkiye)

Ayla ŞENER, (Hacettepe Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, Ankara, Türkiye)

Aslı ÖZKÖK TÜYLÜ, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Kadriye SORKUN, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Bekir SALİH (Hacettepe Üniverstesi, Fen Fakültesi, Kimya Bölümü, Ankara, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2011 Cilt: 35 Sayı: 4 Sayfa Aralığı: 503 - 511 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes

Öz:

Bu çalışmanın amacı, Türkiye’nin çeşitli coğrafi k bölgelerinden toplanan 25 propolis örneğinin, iki gıda patojenine (Salmonella Enteritidis ATCC 13076 ve Listeria monocytogenes ATCC 1462) karşı antibakteriyal aktivitelerini araştırmaktır. Propolis örneklerinin etil alkol ekstraktlarının (EEP) kimyasal kompozisyonları, kütle spektorometresine birleştirilmiş gaz kromatografi si ile belirlenmiştir. EEP örneklerinin ana bileşimleri fl avonoidler, aromatik asit esterleri, aromatik alkoller, aromatik asitler, alifatik karboksilik asitler, terpenler ve alifatik karboksilik asit esterleri olarak tespit edilmiştir. EEP örneklerinin antibakteriyal aktiviteleri, 1:10 ve 1:100 (v/v) şeklinde iki farklı seyreltmesi denenmiştir. EEP örneklerinin hepsi 1:10 seyreltmede denenen bakteri suşlarına karşı yüksek antibakteriyal aktivite göstermiş ve inkübasyon sonrasında canlı bakteri varlığı belirlenmemiştir. S. Enteritidis suşunun, 1:100 seyreltme oranında EEP örneklerine karşı dirençli olduğu gözlenmiştir. Ancak, 1:100 seyreltme oranında EEP örneklerinin çoğunun L. monocytogenes suşuna karşı yüksek antibakteriyal aktiviteye sahip olduğu görülmüştür. Bazı EEP örnekleri 1:100 seyreltme oranında ortamdaki canlı L. monocytogenes hücrelerinin tümünü öldürürken, bazı EEP örnekleri bu suş üzerinde 1-6 log düzeyinde azalmalar gerçekleştirmiştir. Propolisin antibakteriyal aktivitesi, kimyasal kompozisyonuna ve bakteri türüne bağlıdır. Propolis örneklerinin EEP konsantrasyonuna bağlı olarak, gram-pozitif suşa karşı önemli düzeyde antibakteriyal aktiviteye sahip olduğu tespit edilirken, gram-negatif suşa karşı sınırlı düzeyde aktivite gösterdiği belirlenmiştir.

Anahtar Kelime: Salmonella Salmonella enteritidis antibakteriyel özellikler mikrobiyal bulaşıklık gıda bulaşıklılığı gıda hijyeni propolis Listeria monocytogenes arı ürünleri

Konular: Biyoloji

Türkiye’nin farklı coğrafik bölgelerinden toplanan propolis örneklerinin Salmonella enteritidis ve Listeria monocytogenes’e karşı antibakteriyal aktiviteleri

Öz:

Th e aim of the present study was to investigate the antibacterial activities of 25 propolis samples collected from various geographical regions of Turkey against 2 food-borne pathogens, Salmonella Enteritidis ATCC 13076 and Listeria monocytogenes ATCC 1462. Th e chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. Th e main components of EEP samples were fl avonoids, aromatic acid esters, aromatic alcohols, aromatic acids, aliphatic carboxylic acids, terpens, and aliphatic carboxylic acid esters. Antibacterial activities of the EEP samples were tested at 2 diff erent dilutions of 1:10 and 1:100 (v/v). All EEP samples at 1:10 dilution showed high antibacterial activity against the test bacterial strains and no viable bacteria were determined aft er incubation. Th e S. Enteritidis strain was found to be resistant toward EEP samples at 1:100 dilution ratios. However, most of the EEP at 1:100 dilution ratios had high antibacterial activity against the L. monocytogenes strain. While some of EEP at 1:100 dilution ratios killed all viable cells of L. monocytogenes, some EEP carried out 1-6 log reductions in the viable cells of this strain. Antibacterial activity of propolis depends on the chemical composition and types of bacteria. Th e propolis samples had a marked antibacterial action against the gram-positive strain and limited activity against gram-negative one depending on EEP concentration.

Anahtar Kelime: propolis Listeria monocytogenes hive products Salmonella Salmonella enteritidis antibacterial properties microbial contamination food contamination food hygiene

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TEMİZ A, ŞENER A, TÜYLÜ A, SORKUN K, Salih B (2011). Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. , 503 - 511.
Chicago	TEMİZ Ayhan,ŞENER Ayla,TÜYLÜ Aslı özkök,SORKUN KADRİYE,Salih Bekir Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. (2011): 503 - 511.
MLA	TEMİZ Ayhan,ŞENER Ayla,TÜYLÜ Aslı özkök,SORKUN KADRİYE,Salih Bekir Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. , 2011, ss.503 - 511.
AMA	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. . 2011; 503 - 511.
Vancouver	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. . 2011; 503 - 511.
IEEE	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B "Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes." , ss.503 - 511, 2011.
ISNAD	TEMİZ, Ayhan vd. "Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes". (2011), 503-511.

APA	TEMİZ A, ŞENER A, TÜYLÜ A, SORKUN K, Salih B (2011). Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology, 35(4), 503 - 511.
Chicago	TEMİZ Ayhan,ŞENER Ayla,TÜYLÜ Aslı özkök,SORKUN KADRİYE,Salih Bekir Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology 35, no.4 (2011): 503 - 511.
MLA	TEMİZ Ayhan,ŞENER Ayla,TÜYLÜ Aslı özkök,SORKUN KADRİYE,Salih Bekir Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology, vol.35, no.4, 2011, ss.503 - 511.
AMA	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology. 2011; 35(4): 503 - 511.
Vancouver	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes. Turkish Journal of Biology. 2011; 35(4): 503 - 511.
IEEE	TEMİZ A,ŞENER A,TÜYLÜ A,SORKUN K,Salih B "Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes." Turkish Journal of Biology, 35, ss.503 - 511, 2011.
ISNAD	TEMİZ, Ayhan vd. "Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella enteritidis and Listeria monocytogenes". Turkish Journal of Biology 35/4 (2011), 503-511.