Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model

Genç, Sıdıka

doi:10.14744/ejmi.2023.14125

Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model

Sıdıka GENÇ (Bilecik Şeyh Edebali Üniversitesi Tıp Fakültesi Tıbbi Farmakoloji Anabilim Dalı, Bilecik, Türkiye)

Eurasian Journal of Medical Investigation

4 0

Yıl: 2023 Cilt: 7 Sayı: 1 Sayfa Aralığı: 74 - 81 Metin Dili: İngilizce DOI: 10.14744/ejmi.2023.14125 İndeks Tarihi: 02-06-2023

Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model

Öz:

Objectives: This study was conducted to research the protective effects of Morinda citrifolia (Noni) against the neuro- toxicity, of 6-Hydroxydopamine (6-OHDA)-induced in the neuroblastoma (SH-SY5Y) cells model. Methods and Results: 200 μM 6-OHDA were applied on SH-SY5Y cells under in vitro conditions to model Parkinson’s Disease. Before the 6-OHDA application, it was treated with different doses of Noni extract (5, 10, 25, and 50 μg/mL). Cell viability was measured with routinely used 3-(4,5-Dimethyl Thiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and cytotoxic activity was determined by lactate dehydrogenase (LDH) assays. Besides cell viability, oxidative stress was evaluated by total antioxidant capacity (TAC), total oxidant status, superoxide dismutase assay, malondialdehyde, and glutathione reductase. The effect of Noni on Bax/Bcl2 gene regulation in SH-SY5Y cells was investigated by real-time PCR. 6-OHDA 200 μM decreased cell viability by approximately 60% in SH-SY5Y cells and increased LDH level. With 50 μg/mL Noni treatment, cell viability increased to 88.32%, while the LDH level decreased. Similarly, Noni re-regulated Bax and Bcl-2 level, modified 6-OHDA, and decreased oxidative stress levels (p<0.05 and p<0.001). Conclusion: These findings prove that noni exhibits a neuroprotective effect against 6-OHDA induction, through Bax/ Bc-l2 gene regulation and decreasing oxidative stress.

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Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Bibliyografik

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APA	Genç S (2023). Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. , 74 - 81. 10.14744/ejmi.2023.14125
Chicago	Genç Sıdıka Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. (2023): 74 - 81. 10.14744/ejmi.2023.14125
MLA	Genç Sıdıka Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. , 2023, ss.74 - 81. 10.14744/ejmi.2023.14125
AMA	Genç S Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. . 2023; 74 - 81. 10.14744/ejmi.2023.14125
Vancouver	Genç S Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. . 2023; 74 - 81. 10.14744/ejmi.2023.14125
IEEE	Genç S "Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model." , ss.74 - 81, 2023. 10.14744/ejmi.2023.14125
ISNAD	Genç, Sıdıka. "Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model". (2023), 74-81. https://doi.org/10.14744/ejmi.2023.14125

APA	Genç S (2023). Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. Eurasian Journal of Medical Investigation, 7(1), 74 - 81. 10.14744/ejmi.2023.14125
Chicago	Genç Sıdıka Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. Eurasian Journal of Medical Investigation 7, no.1 (2023): 74 - 81. 10.14744/ejmi.2023.14125
MLA	Genç Sıdıka Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. Eurasian Journal of Medical Investigation, vol.7, no.1, 2023, ss.74 - 81. 10.14744/ejmi.2023.14125
AMA	Genç S Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. Eurasian Journal of Medical Investigation. 2023; 7(1): 74 - 81. 10.14744/ejmi.2023.14125
Vancouver	Genç S Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model. Eurasian Journal of Medical Investigation. 2023; 7(1): 74 - 81. 10.14744/ejmi.2023.14125
IEEE	Genç S "Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model." Eurasian Journal of Medical Investigation, 7, ss.74 - 81, 2023. 10.14744/ejmi.2023.14125
ISNAD	Genç, Sıdıka. "Morinda Citrifolia Modulate Bax/Bcl-2 Ratio to Inhibition of Apoptosis Induced By 6 OHDA – in SY-5Y Cells Model". Eurasian Journal of Medical Investigation 7/1 (2023), 74-81. https://doi.org/10.14744/ejmi.2023.14125