Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma

Aksoy, Seçil; Tunca, Berrin; Tekin, Cagla; Tezcan, Gulcin; Ercelik, melis

doi:10.4274/tjps.galenos.2022.57639

Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma

melis Ercelik, (Bursa Uludağ Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Bursa, Türkiye)

Berrin TUNCA, (Bursa Uludağ Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Bursa, Türkiye)

Seçil AK AKSOY, (Bursa Uludağ Üniversitesi, İnegöl Meslek Yüksekokulu, Bursa, Türkiye)

Çağla TEKİN, (Bursa Uludağ Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Bursa, Türkiye)

Gulcin Tezcan (Bursa Uludağ Üniversitesi, Diş Hekimliği Fakültesi, Temel Bilimler Bölümü, Bursa, Türkiye)

Turkish Journal of Pharmaceutical Sciences

4 1

Yıl: 2023 Cilt: 20 Sayı: 2 Sayfa Aralığı: 68 - 77 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2022.57639 İndeks Tarihi: 02-06-2023

Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma

Öz:

Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB). Materials and Methods: A senescence β-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-κB1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology. Results: TMZ increased the senescence rate of GB cells (p<0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p<0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-κB1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p<0.0001). Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ercelik m, Tunca B, Aksoy S, Tekin C, Tezcan G (2023). Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. , 68 - 77. 10.4274/tjps.galenos.2022.57639
Chicago	Ercelik melis,Tunca Berrin,Aksoy Seçil,Tekin Cagla,Tezcan Gulcin Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. (2023): 68 - 77. 10.4274/tjps.galenos.2022.57639
MLA	Ercelik melis,Tunca Berrin,Aksoy Seçil,Tekin Cagla,Tezcan Gulcin Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. , 2023, ss.68 - 77. 10.4274/tjps.galenos.2022.57639
AMA	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. . 2023; 68 - 77. 10.4274/tjps.galenos.2022.57639
Vancouver	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. . 2023; 68 - 77. 10.4274/tjps.galenos.2022.57639
IEEE	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G "Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma." , ss.68 - 77, 2023. 10.4274/tjps.galenos.2022.57639
ISNAD	Ercelik, melis vd. "Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma". (2023), 68-77. https://doi.org/10.4274/tjps.galenos.2022.57639

APA	Ercelik m, Tunca B, Aksoy S, Tekin C, Tezcan G (2023). Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. Turkish Journal of Pharmaceutical Sciences, 20(2), 68 - 77. 10.4274/tjps.galenos.2022.57639
Chicago	Ercelik melis,Tunca Berrin,Aksoy Seçil,Tekin Cagla,Tezcan Gulcin Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. Turkish Journal of Pharmaceutical Sciences 20, no.2 (2023): 68 - 77. 10.4274/tjps.galenos.2022.57639
MLA	Ercelik melis,Tunca Berrin,Aksoy Seçil,Tekin Cagla,Tezcan Gulcin Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. Turkish Journal of Pharmaceutical Sciences, vol.20, no.2, 2023, ss.68 - 77. 10.4274/tjps.galenos.2022.57639
AMA	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. Turkish Journal of Pharmaceutical Sciences. 2023; 20(2): 68 - 77. 10.4274/tjps.galenos.2022.57639
Vancouver	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma. Turkish Journal of Pharmaceutical Sciences. 2023; 20(2): 68 - 77. 10.4274/tjps.galenos.2022.57639
IEEE	Ercelik m,Tunca B,Aksoy S,Tekin C,Tezcan G "Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma." Turkish Journal of Pharmaceutical Sciences, 20, ss.68 - 77, 2023. 10.4274/tjps.galenos.2022.57639
ISNAD	Ercelik, melis vd. "Olea europaea L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma". Turkish Journal of Pharmaceutical Sciences 20/2 (2023), 68-77. https://doi.org/10.4274/tjps.galenos.2022.57639