First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye
Yıl: 2023 Cilt: 13 Sayı: 1 Sayfa Aralığı: 57 - 65 Metin Dili: İngilizce DOI: 10.17714/gumusfenbil.1179361 İndeks Tarihi: 19-07-2023
First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye
Öz: The study set out to look at the clinical strain of A. faecalis's antibiotic susceptibility pattern. Both biochemical and molecular approaches were used to identify A.faecalis. The VITEK compact system was used to analyze the strain's antibiotic susceptibility profile. The use of PCR was evaluated to determine the presence of resistance genes. It was also determined whether resistance determinants could be transferred. It was determined that A. feacalis showed resistance to imipenem, cefepime, ceftazidime, gentamicin, amikacin, netilmicin, tobramicin, ciprofloxacin, and levofloxacin. The blaVIM-4-like gene was present in this strain, according to the results of the PCR and DNA sequencing studies. In contrast to VIM-4, this blaVIM-4-like gene showed one amino acid change (Ala265Val).Filter-mating experiments showed that the blaVIM-4-like gene cannot be transferred from A. faecalis to E. coli. In this study, This research, demonstrated the presence of the blaVIM-4-like gene for the first time in A. faecalis from Turkey.
Anahtar Kelime: Türkiye'den izole edilen qnr ve blaVIM-4-like üreten klinik Alcaligenes faecalis’in ilk raporu
Öz: Çalışmanın amacı, A. faecalis klinik suşunun antibiyotik duyarlılık paterninin araştırmaktır. A. faecalis hem biyokimyasal hem de moleküler yöntemlerle tanımlanmıştır. VITEK kompakt sistemi, suşun antibiyotik duyarlılık profilini analiz etmek için kullanıldı. Direnç genlerinin belirlenmesi, PCR yöntemi kullanılarak değerlendirildi. Ayrıca, direnç belirleyicilerinin aktarılabilirliği araştırıldı. A. feacalis'in imipenem, sefepim, seftazidim, gentamisin, amikasin, netilmisin, tobramisin, siprofloksasin ve levofloksasine direnç gösterdiği belirlendi. PCR ve DNA dizi analizleri, bu suşun blaVIM-4 benzeri gene sahip olduğunu ortaya çıkardı. Bu blaVIM-4-like gen, VIM-4'e kıyasla bir amino asit ikamesi (Ala265Val) sergiledi. Filtre eşleştirme deneyleri, blaVIM-4-like genin A. faecalis'ten E. coli'ye aktarılamayacağını gösterdi. Bu çalışmada, Türkiye'den A. faecalis'te ilk kez blaVIM-4-like genin varlığı ortaya konmuştur.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | akyildiz e, SARAL SARIYER A, Köse T, M, BERIS F, ÖZAD DÜZGÜN A (2023). First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. , 57 - 65. 10.17714/gumusfenbil.1179361 |
| Chicago | akyildiz esma,SARAL SARIYER AYSEGÜL,Köse Tuba, Mikail,BERIS FATIH SABAN,ÖZAD DÜZGÜN AZER First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. (2023): 57 - 65. 10.17714/gumusfenbil.1179361 |
| MLA | akyildiz esma,SARAL SARIYER AYSEGÜL,Köse Tuba, Mikail,BERIS FATIH SABAN,ÖZAD DÜZGÜN AZER First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. , 2023, ss.57 - 65. 10.17714/gumusfenbil.1179361 |
| AMA | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. . 2023; 57 - 65. 10.17714/gumusfenbil.1179361 |
| Vancouver | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. . 2023; 57 - 65. 10.17714/gumusfenbil.1179361 |
| IEEE | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A "First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye." , ss.57 - 65, 2023. 10.17714/gumusfenbil.1179361 |
| ISNAD | akyildiz, esma vd. "First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye". (2023), 57-65. https://doi.org/10.17714/gumusfenbil.1179361 |
| APA | akyildiz e, SARAL SARIYER A, Köse T, M, BERIS F, ÖZAD DÜZGÜN A (2023). First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 13(1), 57 - 65. 10.17714/gumusfenbil.1179361 |
| Chicago | akyildiz esma,SARAL SARIYER AYSEGÜL,Köse Tuba, Mikail,BERIS FATIH SABAN,ÖZAD DÜZGÜN AZER First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 13, no.1 (2023): 57 - 65. 10.17714/gumusfenbil.1179361 |
| MLA | akyildiz esma,SARAL SARIYER AYSEGÜL,Köse Tuba, Mikail,BERIS FATIH SABAN,ÖZAD DÜZGÜN AZER First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, vol.13, no.1, 2023, ss.57 - 65. 10.17714/gumusfenbil.1179361 |
| AMA | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2023; 13(1): 57 - 65. 10.17714/gumusfenbil.1179361 |
| Vancouver | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2023; 13(1): 57 - 65. 10.17714/gumusfenbil.1179361 |
| IEEE | akyildiz e,SARAL SARIYER A,Köse T, M,BERIS F,ÖZAD DÜZGÜN A "First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye." Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 13, ss.57 - 65, 2023. 10.17714/gumusfenbil.1179361 |
| ISNAD | akyildiz, esma vd. "First report of qnr and blaVIM-4-like producing clinical Alcaligenes faecalis isolated in Türkiye". Gümüşhane Üniversitesi Fen Bilimleri Dergisi 13/1 (2023), 57-65. https://doi.org/10.17714/gumusfenbil.1179361 |
