Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function

Ihsan YOZGAT, (Acıbadem Mehmet Ali Aydınlar Üniversitesi, Sağlık Bilimleri Enstitüsü, Tıbbi Biyoteknoloji Anabilim Dalı, İstanbul, Türkiye)
Betul ŞAHİN, (Acıbadem Labmed Klinik Laboratuvarları, İstanbul, Türkiye)
Neslihan YILDIRIM SARAL, (Acıbadem Labmed Klinik Laboratuvarları, İstanbul, Türkiye)
Zafer Banu ULUSOY, (Acıbadem Bursa Hastanesi, Merkez Klinik Laboratuvarı, Bursa, Türkiye)
Meltem KİLERCİK, (Acıbadem Bursa Hastanesi, Merkez Klinik Laboratuvarı, Bursa, Türkiye)
Huseyin ÇELİK, (Acıbadem Bursa Hastanesi, Nefroloji ve Organ Nakli Bölümü, Bursa, Türkiye)
Mahmut Esat DANIŞOĞLU, (Acıbadem Bursa Hastanesi, Üroloji ve Organ Nakli Kliniği, Bursa, Türkiye)
Soner DUMAN, (Ege Üniversitesi, Tıp Fakültesi, Nefroloji Anabilim Dalı, İzmir, Türkiye)
Bülent OKTAY, (Acıbadem Bursa Hastanesi, Üroloji ve Organ Nakli Kliniği, Bursa, Türkiye)
Mustafa SERTESER, (Acıbadem Bursa Hastanesi, Merkez Klinik Laboratuvarı, Bursa, Türkiye)
Ahmet Tarik BAYKAL,
Journal of research in pharmacy (online)
27 0
Yıl: 2023 Cilt: 27 Sayı: 4 Sayfa Aralığı: 1673 - 1686 Metin Dili: İngilizce DOI: 10.29228/jrp.451 İndeks Tarihi: 28-07-2023

Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function

Öz:
The ability to monitor patients plays a major role in the success of kidney transplants. However, transplant monitoring still depends on relatively outdated, inadequate technologies. The aim of this study was to reveal the metabolomic profile of the kidney allograft using the metabolomic screening technique and to identify specific eGFRbased biomarkers to monitor individuals with different levels of post-transplantation graft dysfunction. In the current study, urine samples from 131 unique kidney transplant recipients were collected and analyzed by ultra-high performance liquid chromatography and benchtop QTof mass spectrometer (Xevo G2 XS QTof). Acquired data were first pre-processed by Progenesis QI 2.3 (Nonlinear Dynamics, Waters, UK). Putative annotation was performed against the HMDB database following multivariate statistical analysis. Post-transplant biomarker panels that can distinguish stages of renal dysfunction were created by combining the significant markers and taking their ratios. Overall, 8 metabolites were significantly altered within three groups of kidney transplant recipients:4,5-Dihydroorotic acid, N2- Succinyl-L-glutamic acid 5-semialdehyde, Valyl-Arginine, Pantothenic acid, L-phenylalanyl-L-hydroxyproline, MG(0:0/24:0/0:0), QYNAD and 12-Hydroxy-13-O-D-glucuronoside-octadec-9Z-enoate as biomarker candidates (p<0.05). The ratio of 4,5-Dihydroorotic acid to Pantothenic acid (panel-1) can be used to monitor kidney function. Specifically, these metabolite ratios were found to be more sensitive to changes in kidney function than panel-2, which consisted of 7 metabolites, excluding QYNAD, of the 8 major metabolites. Our results may contribute to the monitoring of kidney transplant patients based on post-transplant eGFR-based kidney function stages, thus providing a method for the early evaluation and monitoring of the kidney transplant recipient after transplantation for kidney transplant patient management.
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APA YOZGAT I, ŞAHİN B, SARAL N, ULUSOY Z, KİLERCİK M, ÇELİK H, DANIŞOĞLU M, DUMAN S, OKTAY B, Serteser M, BAYKAL A (2023). Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. , 1673 - 1686. 10.29228/jrp.451
Chicago YOZGAT Ihsan,ŞAHİN Betul,SARAL Neslihan YILDIRIM,ULUSOY Zafer Banu,KİLERCİK Meltem,ÇELİK Huseyin,DANIŞOĞLU Mahmut Esat,DUMAN SONER,OKTAY Bülent,Serteser Mustafa,BAYKAL Ahmet Tarik Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. (2023): 1673 - 1686. 10.29228/jrp.451
MLA YOZGAT Ihsan,ŞAHİN Betul,SARAL Neslihan YILDIRIM,ULUSOY Zafer Banu,KİLERCİK Meltem,ÇELİK Huseyin,DANIŞOĞLU Mahmut Esat,DUMAN SONER,OKTAY Bülent,Serteser Mustafa,BAYKAL Ahmet Tarik Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. , 2023, ss.1673 - 1686. 10.29228/jrp.451
AMA YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. . 2023; 1673 - 1686. 10.29228/jrp.451
Vancouver YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. . 2023; 1673 - 1686. 10.29228/jrp.451
IEEE YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A "Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function." , ss.1673 - 1686, 2023. 10.29228/jrp.451
ISNAD YOZGAT, Ihsan vd. "Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function". (2023), 1673-1686. https://doi.org/10.29228/jrp.451
APA YOZGAT I, ŞAHİN B, SARAL N, ULUSOY Z, KİLERCİK M, ÇELİK H, DANIŞOĞLU M, DUMAN S, OKTAY B, Serteser M, BAYKAL A (2023). Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. Journal of research in pharmacy (online), 27(4), 1673 - 1686. 10.29228/jrp.451
Chicago YOZGAT Ihsan,ŞAHİN Betul,SARAL Neslihan YILDIRIM,ULUSOY Zafer Banu,KİLERCİK Meltem,ÇELİK Huseyin,DANIŞOĞLU Mahmut Esat,DUMAN SONER,OKTAY Bülent,Serteser Mustafa,BAYKAL Ahmet Tarik Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. Journal of research in pharmacy (online) 27, no.4 (2023): 1673 - 1686. 10.29228/jrp.451
MLA YOZGAT Ihsan,ŞAHİN Betul,SARAL Neslihan YILDIRIM,ULUSOY Zafer Banu,KİLERCİK Meltem,ÇELİK Huseyin,DANIŞOĞLU Mahmut Esat,DUMAN SONER,OKTAY Bülent,Serteser Mustafa,BAYKAL Ahmet Tarik Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. Journal of research in pharmacy (online), vol.27, no.4, 2023, ss.1673 - 1686. 10.29228/jrp.451
AMA YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. Journal of research in pharmacy (online). 2023; 27(4): 1673 - 1686. 10.29228/jrp.451
Vancouver YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function. Journal of research in pharmacy (online). 2023; 27(4): 1673 - 1686. 10.29228/jrp.451
IEEE YOZGAT I,ŞAHİN B,SARAL N,ULUSOY Z,KİLERCİK M,ÇELİK H,DANIŞOĞLU M,DUMAN S,OKTAY B,Serteser M,BAYKAL A "Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function." Journal of research in pharmacy (online), 27, ss.1673 - 1686, 2023. 10.29228/jrp.451
ISNAD YOZGAT, Ihsan vd. "Untargeted urinary metabolomic profiling in post-kidney transplant with different levels of kidney function". Journal of research in pharmacy (online) 27/4 (2023), 1673-1686. https://doi.org/10.29228/jrp.451
Sayfa Oluşturulma Tarihi
24-04-2024 8:38

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