Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity

SENER, SILA OZLEM; badem, merve; ÇATALBAŞ, Mehmet; KANBOLAT, Şeyda; Özgen, Ufuk; Ulaş çolak, Nevin

doi:10.15671/hjbc.1196913

Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity

Sıla Özlem SENER, (Sağlık Bilimleri Üniversitesi, Eczacılık Fakültesi, Farmakanozi Anabilim Dalı, Ankara, Türkiye)

Merve BADEM, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Trabzon, Türkiye)

Mehmet CATALBAS, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Trabzon, Türkiye)

Şeyda KANBOLAT, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Trabzon, Türkiye)

Ufuk OZGEN, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Farmakanozi Anabilim Dalı, Trabzon, Türkiye)

Nevin ULAS COLAK (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Analitik Kimya Anabilim Dalı, Trabzon, Türkiye)

Hacettepe Journal of Biology and Chemistry

2 0

Yıl: 2023 Cilt: 51 Sayı: 3 Sayfa Aralığı: 251 - 258 Metin Dili: İngilizce DOI: 10.15671/hjbc.1196913 İndeks Tarihi: 17-10-2023

Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity

Öz:

Nowadays, one of the therapeutic approaches for obesity is the use pancreatic lipase inhibitors which reduce the digestion and absorption of fats. Most research indicates that natural sources which have a lipase inhibitory effect, may be utilized to treat obesity. Verbascum pyramidatum is one of the potential natural sources for obesity, and it has been demonstrated to have anti-inflammatory, antidiabetic, and regulatory effects on lipid metabolism. With this study, V. pyramidatum's potential lipase inhibitor effect, it is aimed to reveal its value in the treatment of obesity. In vitro spectroscopic method was used to determine the lipase inhibitory effect of V. pyramidatum. The quantitative investigation of V. pyramidatum's phenolic metabolites with antiobesity activity was carried out utilizing the Reverse Phase-High Performance Liquid Chromatography method. In this reported study, it was proven that extract and all fractions had an impact that inhibited lipase, with the ethyl acetate extract showing the highest inhibitory effect. Additionally, it was revealed through HPLC analysis that the species included p-OH benzoic acid, coumaric acid, quercetin, sinapic acid, and syringaldehyde. It has been demonstrated that V. pyramidatum may be a promising candidate for obesity treatment, but further investigations are required to use it as a therapeutic agent.

Anahtar Kelime: Lipase Obesity RP-HPLC Verbascum pyramidatum

Verbascum pyramidatum Bieb.’in Obezitedeki Tedavi Edici Değerinin Araştırılması

Öz:

Obezite için günümüzde geçerliliğini koruyan tedavi yaklaşımlarından biri, lipitlerin sindirimini ve absorpsiyonunu azaltan pankreatik lipaz inhibitörlerinin kullanılmasıdır. Lipaz inhibitör etki potansiyeli bulunan doğal kaynakların obezite tedavisinde kullanılabileceği ileri sürülmektedir. Anti-enflamatuvar, antidiyabetik ve lipit metabolizması üzerinde düzenleyici etkileri kanıt- lanmış Verbascum pyramidatum, obezite tedavisinde kullanılma potansiyeli bulunan doğal kaynaklardan biridir. Bu çalışma ile, V. pyramidatum’un potansiyel lipaz inhibitör etkisi üzerinden, obezite tedavisindeki değerinin ortaya çıkarılması amaçlanmıştır. V. pyramidatum’un lipaz inhibitör etkisi belirlemek için in vitro spektroskopik yöntemden yararlanılmıştır. Ters Faz Yüksek Basınçlı Sıvı Kromatografisi yöntemi ile V. pyramidatum içeriğindeki antiobezite aktiviteye sahip fenolik karakterde bazı metabolitlerin kantitatif analizi gerçekleştirilmiştir. In vitro lipaz inhibitör etki belirleme çalışmaları sonucunda, V. pyramidatum’un farklı çözücü sistemleriyle hazırlanan ekstre ve fraksiyonlarının lipaz inhibitör etkiye sahip olduğu belirlenmiştir. En yüksek lipaz inhibitör etki etil asetat fraksiyonunda gözlenmiştir. YPSK analizinde türün p-OH benzoik asit; kumarik asit, kersetin, sinapik asit ve syringaldehit içerdiği tespit edilmiştir. Bu çalışma ile V. pyramidatum’un obezite tedavisi için umut verici bir doğal kaynak olduğu ortaya çıkarıl- makla birlikte terapötik bir ajan olarak kullanımı daha ileri araştırmalar gerektirmektedir.

Anahtar Kelime: Lipaz Obezite TF-YBSK Verbascum pyramidatum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SENER S, badem m, ÇATALBAŞ M, KANBOLAT Ş, Özgen U, Ulaş çolak N (2023). Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. , 251 - 258. 10.15671/hjbc.1196913
Chicago	SENER SILA OZLEM,badem merve,ÇATALBAŞ Mehmet,KANBOLAT Şeyda,Özgen Ufuk,Ulaş çolak Nevin Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. (2023): 251 - 258. 10.15671/hjbc.1196913
MLA	SENER SILA OZLEM,badem merve,ÇATALBAŞ Mehmet,KANBOLAT Şeyda,Özgen Ufuk,Ulaş çolak Nevin Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. , 2023, ss.251 - 258. 10.15671/hjbc.1196913
AMA	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. . 2023; 251 - 258. 10.15671/hjbc.1196913
Vancouver	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. . 2023; 251 - 258. 10.15671/hjbc.1196913
IEEE	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N "Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity." , ss.251 - 258, 2023. 10.15671/hjbc.1196913
ISNAD	SENER, SILA OZLEM vd. "Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity". (2023), 251-258. https://doi.org/10.15671/hjbc.1196913

APA	SENER S, badem m, ÇATALBAŞ M, KANBOLAT Ş, Özgen U, Ulaş çolak N (2023). Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. Hacettepe Journal of Biology and Chemistry, 51(3), 251 - 258. 10.15671/hjbc.1196913
Chicago	SENER SILA OZLEM,badem merve,ÇATALBAŞ Mehmet,KANBOLAT Şeyda,Özgen Ufuk,Ulaş çolak Nevin Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. Hacettepe Journal of Biology and Chemistry 51, no.3 (2023): 251 - 258. 10.15671/hjbc.1196913
MLA	SENER SILA OZLEM,badem merve,ÇATALBAŞ Mehmet,KANBOLAT Şeyda,Özgen Ufuk,Ulaş çolak Nevin Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. Hacettepe Journal of Biology and Chemistry, vol.51, no.3, 2023, ss.251 - 258. 10.15671/hjbc.1196913
AMA	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. Hacettepe Journal of Biology and Chemistry. 2023; 51(3): 251 - 258. 10.15671/hjbc.1196913
Vancouver	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity. Hacettepe Journal of Biology and Chemistry. 2023; 51(3): 251 - 258. 10.15671/hjbc.1196913
IEEE	SENER S,badem m,ÇATALBAŞ M,KANBOLAT Ş,Özgen U,Ulaş çolak N "Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity." Hacettepe Journal of Biology and Chemistry, 51, ss.251 - 258, 2023. 10.15671/hjbc.1196913
ISNAD	SENER, SILA OZLEM vd. "Investigation of the Therapeutic Value of Verbascum pyramidatum Bieb. for Obesity". Hacettepe Journal of Biology and Chemistry 51/3 (2023), 251-258. https://doi.org/10.15671/hjbc.1196913