Object: In this study, we aimed to investigate the data related with the humoral pathology theory in Chagatai Turkish medical works.Materials and Methods: Six texts written in Chagatai Turkish were examined in the study. The study was performed between January 2020 and May 2020. Congress presentations, journal articles, master and doctorate theses, and books which were prepared about İbn-i Kutluk Molla Toh Niyaz Ahond-Nıng Tıbb Kitabı, Ṭabīblik kitābï, Ṭabı̇̄bçılıḳ, Tıb-Nâme-i Türkî, Risāle-i Ṭıb and Müfredat-ı tıb were used as reference source for investigation.Results: At the end of the study, it was seen that information related to humoral pathology theory was present in Chagatai Turkish medical works written in different time periods. The conception in these works are four elements humoral pathology theory which was originated from Ancient Greek and was been the main medical concept of Islamic Medicine and Ottoman Medicine thereafter. Since only a short summary of the contents of Risāle-i Ṭıb ve Müfredat-ı tıb were published in reference works, it couldn’t be reached the full texts of these works. However, there are detailed information about it both in theoretical parts and in diagnosis and treatment parts of the works which were published as full texts. Conclusion: As a result, the main medical concept in the Chagatai Turkish medical works is detected as four elements humoral pathology conception. When evaluated together with the information about Islamic religion in the content of the texts, the obtained data supports the idea that one of the important reasons affecting the medical concept is religion.