The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

YORGANCILAR, MUSTAFA; ERİŞEN, Semiha; ATALAY, Emine

The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

Semiha ERİŞEN, (Selçuk Üniversitesi, Ahmet Keleşoğlu Eğitim Fakültesi, Biyoloji Bölümü, Konya, Türkiye)

Emine ATALAY, (Selçuk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Konya, Türkiye)

Mustafa YORGANCILAR (Selçuk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Konya, Türkiye)

Turkish Journal of Botany

0 0

Yıl: 2011 Cilt: 35 Sayı: 5 Sayfa Aralığı: 521 - 526 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey

Öz:

Th idiazuron (TDZ) sitokinin benzeri bileşiklerden biridir ve son yıllarda sürgün rejenerasyon sistemlerinde sıklıkla kullanılmaktadır. Bu çalışmada endemik Astragalus cariensis Boiss. bitkisine ait yaprak ve yaprak sapı eksplantlarında TDZ’nin kallus oluşumu ve sürgün rejenerasyonuna etkisi araştırılmıştır. Eksplantlar 0,2, 0,4, 0,6 mg/L TDZ ile bunların 0,2 ve 1,0 mg/L α-naphthaleneacetic acid (NAA) ile kombinasyonlarını içeren Murashige ve Skoog (MS) besin ortamlarında kültüre alınmıştır. Kallus oluşum yüzdesi ve kallus morfolojisi bakımından eksplantlar arasında farklılık gözlenmiştir. Kallus oluşumunda, yaprak sapı eksplantı, sürgün oluşumunda ise yaprak eksplantı daha iyi sonuç vermiştir. TDZ ve NAA içeren ortamlarda kallus oluşumu % 100 olmasına rağmen, sürgün rejenerasyonunda TDZ ve düşük oranda NAA içeren ortamlar daha iyi sonuç vermiştir. En fazla sürgün rejenerasyonu (15 adet/eksplant) 0,4 mg/L TDZ ve 0,2 mg/L NAA içeren ortamda kültüre alınan yaprak eksplantlarından elde edilmiştir. Rejenere sürgünler 0,5 mg/L indole-3-butyric acid (IBA) içeren MS ortamında köklendirilmiş ve köklenen sürgünler dış ortama başarılı bir şekilde aktarılmıştır.

Anahtar Kelime: filiz Astragalus cariensis gençleştirme endemik bitkiler kallus TDZ organ oluşumu tidiazuron

Konular: Biyoloji

Endemik Astragalus cariensis türünün in vitro sürgün gelişimine thidiazuron’un etkisi

Öz:

Thidiazuron (TDZ) is a cytokinin-like substance that has oft en been used for shoot regeneration in recent years. We observed the eff ect of TDZ on callus formation and shoot regeneration from leaf and petiole explants of the endemic species Astragalus cariensis Boiss. Explants were cultured on Murashige and Skoog (MS) media including TDZ (in concentrations of 0.2, 0.4, or 0.6 mg/L) or in combination with α-naphthaleneacetic acid (NAA) (in concentrations of 0.2 or 1.0 mg/L). Signifi cant variations in the frequency of callus formation and the morphogenesis of the callus were obtained depending on the explant type. Petiole explant was determined to be the best for callus formation, but calli from leaf explants were more responsive in terms of shoot formation. MS media including TDZ and NAA showed 100% callus formation, but shoot regeneration was enhanced by TDZ combined with a low concentration of NAA. Th e highest number of shoots, (15 per explant) was induced from leaf explants on MS medium with 0.4 mg/L TDZ and 0.2 mg/L NAA. Regenerated shoots were rooted in MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). Th e rooted plantlets were acclimatised and produced normal plants.

Anahtar Kelime: regeneration endemic plants callus TDZ organogenesis thidiazuron shoots Astragalus cariensis

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Babaoglu M & Yorgancilar M (2000). TDZ-specifi c plant regeneration in salad burnet. Plant Cell Tiss Org 63: 31-34.
Başalma D, Uranbey S, Gürlek D & Özcan S (2008). TDZ-induced plant regeneration in Astragalus cicer L. Afr J Biotechnol 7: 955- 959.
Chamberlain DF & Matthews VA (1970). Astragalus L. In: Davis PH (ed.), Flora of Turkey and the East Aegean Islands, Vol. 3, pp. 49-254. Edinburgh: Edinburgh University Press.
Cho HJ & Widholm JM (2002). Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus. Plant Cell Tiss Org 69: 259-269.
Davis PH, Mill R & Tan K (1988). Astragalus L. In: Davis PH, Mill R, Tan K. (eds.), Flora of Turkey and the East Aegean Islands (Suppl. 1), Vol. 10, pp. 114-124. Edinburgh: Edinburgh University Press.
Duman H & Akan H (2003). New species of Astragalus (sect. Alopecuroidei: Leguminosae) from Turkey. Bot J Linn Soc 143: 201-205.
Duran A & Aytaç Z (2005) Astragalus nezaketae (Fabaceae), a new species from Turkey. Ann Bot Fenn 42: 381-385.
Erisen S, Yorgancilar M, Atalay E & Babaoglu, M (2010). Prolifi c shoot regeneration of Astragalus cariensis Boiss. Plant Cell Tiss Org 100: 229-233.
Ghahremaninejad F & Behçet L (2003) Astragalus subhanensis (Fabaceae), a new species from Turkey. Ann Bot Fenn 40: 209- 211.
Hou SW & Jia JF (2004). High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis. Plant Cell Tiss Org 79: 95-100.
Huetteman CA & Preece JE (1993). Th idiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss Org 33: 105-119.
Hung CY & Xie J (2008). Development of an effi cient plant regeneration system for the selenium-hyperaccumulator Astragalus recemosus and the nonaccumulator Astragalus canadensis. Hortscience 43: 2138-2142.
IUCN (2001). Red List Categories, Version 3.1. Prepared by the IUCN Species Survival Commission. Gland, Switzerland and Cambridge, United Kingdom: IUCN.
Lincy A & Sasikumar B (2010). Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turk J Bot 34: 21-29.
Luo JP & Jia JP (1998a). Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens. Plant Cell Rep 17: 567-570.
Luo JP & Jia JP (1998b). Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall. Plant Cell Rep 17: 313-317.
Martin E, Duran A, Dinç M & Erişen S (2008). Karyotype analyses of four Astragalus L. (Fabaceae) species from Turkey. Phytologia 90: 133-146.
Mirici S (2004). High frequency of adventitious shoot regeneration from leaf and leaf petiole of endemic Astragalus polemoniacus Bunge. Selçuk University Journal of Agricultural Faculty 18: 31- 34.
Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15: 473-497.
Murthy BNS, Murch SJ & Saxena PK (1998). Th idiazuron: A potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Pl 34: 267-275.
Sahai A, Shahzad A & Anis M (2010). High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turk J Bot 34: 11-20.
Turgut-Kara N & Arı S (2008). In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae). Afr J Biotechnol 7: 1250-1255.
Uranbey S, Çöcü S, Sancak C, Parmaksız İ, Khawar KM, Mirici S & Özcan S (2003). Adventitious shoot regeneration in Cicer milkvetch. Biotechnol Biotec Eq 17: 33-37.

APA	ERİŞEN S, ATALAY E, YORGANCILAR M (2011). The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. , 521 - 526.
Chicago	ERİŞEN Semiha,ATALAY Emine,YORGANCILAR MUSTAFA The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. (2011): 521 - 526.
MLA	ERİŞEN Semiha,ATALAY Emine,YORGANCILAR MUSTAFA The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. , 2011, ss.521 - 526.
AMA	ERİŞEN S,ATALAY E,YORGANCILAR M The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. . 2011; 521 - 526.
Vancouver	ERİŞEN S,ATALAY E,YORGANCILAR M The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. . 2011; 521 - 526.
IEEE	ERİŞEN S,ATALAY E,YORGANCILAR M "The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey." , ss.521 - 526, 2011.
ISNAD	ERİŞEN, Semiha vd. "The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey". (2011), 521-526.

APA	ERİŞEN S, ATALAY E, YORGANCILAR M (2011). The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany, 35(5), 521 - 526.
Chicago	ERİŞEN Semiha,ATALAY Emine,YORGANCILAR MUSTAFA The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany 35, no.5 (2011): 521 - 526.
MLA	ERİŞEN Semiha,ATALAY Emine,YORGANCILAR MUSTAFA The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany, vol.35, no.5, 2011, ss.521 - 526.
AMA	ERİŞEN S,ATALAY E,YORGANCILAR M The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany. 2011; 35(5): 521 - 526.
Vancouver	ERİŞEN S,ATALAY E,YORGANCILAR M The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey. Turkish Journal of Botany. 2011; 35(5): 521 - 526.
IEEE	ERİŞEN S,ATALAY E,YORGANCILAR M "The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey." Turkish Journal of Botany, 35, ss.521 - 526, 2011.
ISNAD	ERİŞEN, Semiha vd. "The effect of thidiazuron on the in vitro shoot development of endemic Astragalus cariensis in Turkey". Turkish Journal of Botany 35/5 (2011), 521-526.