Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model

Belhan, Saadet; Huyut, Zübeyir; yildirim, Serkan; ergül erkeç, özlem; özdek, uğur

doi:10.55730/1300-0128.4315

Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model

Saadet BELHAN, (Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Üreme ve Suni Tohumlama Anabilim Dalı, Van, Türkiye)

Zübeyir HUYUT, (Van Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Van, Türkiye)

Serkan YILDIRIM, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Erzurum, Türkiye)

Özlem ERGÜL ERKEÇ, (Van Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Van, Türkiye)

Uğur ÖZDEK (Van Yüzüncü Yıl Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Van, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

3 0

Yıl: 2023 Cilt: 47 Sayı: 5 Sayfa Aralığı: 469 - 477 Metin Dili: İngilizce DOI: 10.55730/1300-0128.4315 İndeks Tarihi: 23-11-2023

Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model

Öz:

Testicular dysfunction, a severe secondary complication of diabetes, negatively affects reproductive health. Ghrelin is a peptide that plays a role in many metabolic events, such as stimulating growth hormones and regulating appetite, blood pressure, and reproduction. Metformin has antiinflammatory and antioxidant properties and is widely used to treat type 2 diabetes. Therefore, in this study, to investigate the effects of ghrelin and metformin on testicular function in experimental diabetic rats given streptozotocin, placed on a high-fat diet, and that had their sperm parameters evaluated, testosterone hormone, caspase 3 and iNOS expression, and histopathological examinations were performed. In total, 38 Wistar albino male rats were randomized into 4 groups (a control group, diabetes group, diabetes + ghrelin group, and diabetes + metformin group) with different numbers. It was determined that spermatozoa concentration, motility, and testosterone levels were lower (p < 0.001), caspase 3 and iNOS expression levels (p < 0.05) were higher, and abnormal sperm rates were higher (p < 0.001) in the diabetic group compared to the other groups. Metformin and ghrelin administered to the diabetes group increased spermatozoa concentration, motility, and testosterone levels (p < 0.001) and decreased caspase 3 and iNOS expression levels, as well as abnormal sperm rates (p < 0.05). Based on these findings, we propose that ghrelin and metformin can be used to protect reproductive health in testicular dysfunction caused by diabetes.

Anahtar Kelime: Caspase 3 iNOS ghrelin metformin sperm

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Belhan S, Huyut Z, yildirim S, ergül erkeç ö, özdek u (2023). Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. , 469 - 477. 10.55730/1300-0128.4315
Chicago	Belhan Saadet,Huyut Zübeyir,yildirim Serkan,ergül erkeç özlem,özdek uğur Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. (2023): 469 - 477. 10.55730/1300-0128.4315
MLA	Belhan Saadet,Huyut Zübeyir,yildirim Serkan,ergül erkeç özlem,özdek uğur Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. , 2023, ss.469 - 477. 10.55730/1300-0128.4315
AMA	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. . 2023; 469 - 477. 10.55730/1300-0128.4315
Vancouver	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. . 2023; 469 - 477. 10.55730/1300-0128.4315
IEEE	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u "Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model." , ss.469 - 477, 2023. 10.55730/1300-0128.4315
ISNAD	Belhan, Saadet vd. "Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model". (2023), 469-477. https://doi.org/10.55730/1300-0128.4315

APA	Belhan S, Huyut Z, yildirim S, ergül erkeç ö, özdek u (2023). Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. Turkish Journal of Veterinary and Animal Sciences, 47(5), 469 - 477. 10.55730/1300-0128.4315
Chicago	Belhan Saadet,Huyut Zübeyir,yildirim Serkan,ergül erkeç özlem,özdek uğur Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. Turkish Journal of Veterinary and Animal Sciences 47, no.5 (2023): 469 - 477. 10.55730/1300-0128.4315
MLA	Belhan Saadet,Huyut Zübeyir,yildirim Serkan,ergül erkeç özlem,özdek uğur Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. Turkish Journal of Veterinary and Animal Sciences, vol.47, no.5, 2023, ss.469 - 477. 10.55730/1300-0128.4315
AMA	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(5): 469 - 477. 10.55730/1300-0128.4315
Vancouver	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model. Turkish Journal of Veterinary and Animal Sciences. 2023; 47(5): 469 - 477. 10.55730/1300-0128.4315
IEEE	Belhan S,Huyut Z,yildirim S,ergül erkeç ö,özdek u "Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model." Turkish Journal of Veterinary and Animal Sciences, 47, ss.469 - 477, 2023. 10.55730/1300-0128.4315
ISNAD	Belhan, Saadet vd. "Evaluation of the effects of ghrelin and metformin on sperm parameters, testosterone hormones, and immunohistochemical and immunofluorescent markers in an experimental diabetes model". Turkish Journal of Veterinary and Animal Sciences 47/5 (2023), 469-477. https://doi.org/10.55730/1300-0128.4315