Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis

CANBOLAT, FADİME; AKINCI KENANOĞLU, Nihan; YÜKSEL, Tuğba Nurcan; Berber, Ahmet Ali

doi:10.16984/saufenbilder.1339199

Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis

Fadime CANBOLAT, (Çanakkale Onsekiz Mart Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Eczacılık Hizmetleri Bölümü, Çanakkale, Türkiye)

Nihan AKINCI KENANOGLU, (Çanakkale Onsekiz Mart Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Çanakkale, Türkiye)

Tugba Nurcan YUKSEL, (Namık Kemal Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Tekirdağ, Türkiye)

Ahmet Ali BERBER (Çanakkale Onsekiz Mart Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Çanakkale, Türkiye)

Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Yıl: 2023 Cilt: 27 Sayı: 6 Sayfa Aralığı: 1185 - 1196 Metin Dili: İngilizce DOI: 10.16984/saufenbilder.1339199 İndeks Tarihi: 24-12-2023

Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis

Öz:

Valproic acid (VPA) is among the most commonly used antiepileptic drugs in childhood and adult epilepsy. Although VPA is well tolerated, it can cause life-threatening side effects. VPA has toxic and genotoxic effects. Antioxidants can reverse drugs' toxic and genotoxic effects. Therefore, our study aimed to evaluate the antigenotoxic protective effect of quercetin (QUE) against VPA genotoxicity by in vitro comet assay analysis method. Comet assay analysis was performed in five different groups. Group I; negative control (Sterile H2O), Group II; positive control (H2O2), Group III; VPA was applied in four different dose ranges, Group IV; QUE was applied in four different dose ranges, Group V; For the simultaneous combined administration of VPA and QUE, three different doses of VPA + four different doses of QUE were administered. Low-dose administration of QUE was more effective in ameliorating the damage caused by low-dose VPA (62.5 μg/ml) administration. It is seen that the genotoxic damage caused by the application of 125 μg/ml VPA can be eliminated by QUE at all doses. It was determined that different doses of QUE exhibited a significant antigenotoxic effect against damage caused by 125 µg/mL VPA (P<0.05). In our study, the curative effect of QUE on DNA damage was determined by in vitro comet analysis. Our analysis results showed that QUE ameliorates VPA-induced genetic damage.

Anahtar Kelime: Valproic acid comet assay in vitro quercetin

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	CANBOLAT F, AKINCI KENANOĞLU N, YÜKSEL T, Berber A (2023). Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. , 1185 - 1196. 10.16984/saufenbilder.1339199
Chicago	CANBOLAT FADİME,AKINCI KENANOĞLU Nihan,YÜKSEL Tuğba Nurcan,Berber Ahmet Ali Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. (2023): 1185 - 1196. 10.16984/saufenbilder.1339199
MLA	CANBOLAT FADİME,AKINCI KENANOĞLU Nihan,YÜKSEL Tuğba Nurcan,Berber Ahmet Ali Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. , 2023, ss.1185 - 1196. 10.16984/saufenbilder.1339199
AMA	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. . 2023; 1185 - 1196. 10.16984/saufenbilder.1339199
Vancouver	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. . 2023; 1185 - 1196. 10.16984/saufenbilder.1339199
IEEE	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A "Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis." , ss.1185 - 1196, 2023. 10.16984/saufenbilder.1339199
ISNAD	CANBOLAT, FADİME vd. "Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis". (2023), 1185-1196. https://doi.org/10.16984/saufenbilder.1339199

APA	CANBOLAT F, AKINCI KENANOĞLU N, YÜKSEL T, Berber A (2023). Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 27(6), 1185 - 1196. 10.16984/saufenbilder.1339199
Chicago	CANBOLAT FADİME,AKINCI KENANOĞLU Nihan,YÜKSEL Tuğba Nurcan,Berber Ahmet Ali Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 27, no.6 (2023): 1185 - 1196. 10.16984/saufenbilder.1339199
MLA	CANBOLAT FADİME,AKINCI KENANOĞLU Nihan,YÜKSEL Tuğba Nurcan,Berber Ahmet Ali Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.27, no.6, 2023, ss.1185 - 1196. 10.16984/saufenbilder.1339199
AMA	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2023; 27(6): 1185 - 1196. 10.16984/saufenbilder.1339199
Vancouver	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2023; 27(6): 1185 - 1196. 10.16984/saufenbilder.1339199
IEEE	CANBOLAT F,AKINCI KENANOĞLU N,YÜKSEL T,Berber A "Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis." Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 27, ss.1185 - 1196, 2023. 10.16984/saufenbilder.1339199
ISNAD	CANBOLAT, FADİME vd. "Evaluation of the Antigenotoxic Effect of Quercetin Against Antiepileptic Drug Genotoxicity by Comet Analysis". Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 27/6 (2023), 1185-1196. https://doi.org/10.16984/saufenbilder.1339199