A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

Özkan, Aysun; ERDOĞAN, Ayse

A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

Aysun ÖZKAN, (Akdeniz Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Antalya, Türkiye)

Ayşe ERDOĞAN (Akdeniz Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Antalya, Türkiye)

Turkish Journal of Biology

5 0

Yıl: 2011 Cilt: 35 Sayı: 6 Sayfa Aralığı: 735 - 742 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

Öz:

Bu çalışmanın amacı, uçucu yağın ve onun iki önemli bileşeni olan karvakrol ve timol’ün hücre canlığı ve hepatoma G2 (Hep G2) hücrelerinde hidrojen peroksidin indüklediği sitotoksisite ve membran hasarına karşı koruyucu etkisinin araştırılmasıdır. Uçucu yağın ve bileşenlerinin antioksidan özelliği, birbirini tamamlayan iki in vitro test sistemi ile ortaya konmuştur, bu testler; DPPH radikalini ortadan kaldırma ve linoleik asit oksidasyonu inhibisyonu testidir. Bitknin toprak üstü organlarının uçucu yağı Clevenger (su buharı distilasyon) cihazı ile izole edilmiş, bileşenler ise gaz kromotografi si/kütle spektrometresi (GC/MS) yöntemi ile analiz edilmiştir. Çalışılan yağ, Türk kekiğinden elde edilmiştir (Origanum onites L.). O. onites’ten elde edilen uçucu yağ yüksek konsantrasyonlarda, kanser hücrelerinin canlılığını azaltmıştır. Hep G2 hücrelerinde, uçucu yağ (IC50: 149,12 μg/mL), karvakrol ve timol’den (IC50: 53,09 ve 60.01 μg/mL) daha az toksik bulunmuştur. Diğer taraft an, Hep G2 hücrelerinin $H _2O _2$ uygulamasından önce, düşük konsantrasyonlarda O. onites uçucu yağı, karvakrol ve timol (<IC50) ile ön uygulamaya maruz bırakılması hücreleri $H _2O _2$ tarafından indüklenen sitotoksisiteye karşı korumuştur. Malondialdehit miktarı, $H _2O _2$’e (IC50 ve IC70) maruz bırakılan hücrelerde artmış, fakat $H _2O _2$’e maruz bırakmadan önce uçucu yağ ve bileşenleri ile ön-uygulamaya maruz bırakılan hücrelerde azalmıştır. Uçucu yağ, timol ve karvakrolden daha yüksek membrane koruyucu etki göstermiştir. Uçucu yağın en önemli üç bileşeni sırasıyla linalool (% 50,53), carvacrol (% 24,52) ve thymol (% 15,66) olarak tanımlanmıştır. Uçucu yağın DPPH radikali süpürücü aktivite (EC50: 80 μg/mL) karvakrol ve timolden daha yüksek bulunmuştur. Uçucu yağın linoleik asit oksidasyonunu inhibisyon oranı (% 40) diğer iki majör bileşene yakındır. Bu bulgular, O. onites’den elde edilen uçucu yağın ve onun iki önemli bileşeninin antioksidan aktiviteye ve karsinogenezi azaltıcı potansiyele sahip olduğunu göstermektedir.

Anahtar Kelime: uçucu yağlar hücre kültürü fenolik bileşikler kekik sitotoksik bileşikler GC-MS neoplazma yemeklik baharatlar karşılaştırmalar uçucu yağ bitkileri timol antineoplastik ajanlar Origanum onites antioksidanlar

Konular: Biyoloji

Origanum onites (Lamiaceae)’den elde edilen uçucu yağın ve iki önemli fenolik bileşeninin antioksidan ve antikanser aktivitelerinin karşılaştırılması

Öz:

The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential oil of O. onites decreased cancer cell viability at higher concentrations. The essential oil was found to be less cytototoxic (IC50: 149.12 μg/mL) than carvacrol and thymol (IC50: 53.09 and 60.01 μg/mL, respectively) for Hep G2 cells. On the other hand, with the O. onites essential oil, carvacrol and thymol protected the cells against $H _2O _2$-induced cytotoxicity when the cells were preincubated with the oil and its components at a lower concentration (<IC50) before H2O2 incubation. The malondialdehyde (MDA) level increased in $H _2O _2$-exposed cells (IC50 and IC70), but decreased in cells preincubated with the essential oil and its components before $H _2O _2$ exposure. The essential oil showed a higher membrane-protective effect than thymol and carvacrol. The 3 major constituents of the essential oil were linalool (50.53%), carvacrol (24.52%), and thymol (15.66%), respectively. The DPPH radical scavenging activity (EC50: 80 μg/mL) of the essential oil was found to be higher than that of carvacrol and thymol. The linoleic acid oxidation inhibition rate of the essential oil (40%) was close to that of its 2 major components. These findings suggest that the essential oil from O. onites and its 2 major components exhibit antioxidant activity and carcinogenesis-reducing potential.

Anahtar Kelime: comparisons essential oil plants thymol antineoplastic agents Origanum onites antioxidants essential oils cell culture phenolic compounds thyme cytotoxic compounds GC-MS neoplasms culinary herbs

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Özkan A, ERDOĞAN A (2011). A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. , 735 - 742.
Chicago	Özkan Aysun,ERDOĞAN Ayse A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. (2011): 735 - 742.
MLA	Özkan Aysun,ERDOĞAN Ayse A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. , 2011, ss.735 - 742.
AMA	Özkan A,ERDOĞAN A A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. . 2011; 735 - 742.
Vancouver	Özkan A,ERDOĞAN A A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. . 2011; 735 - 742.
IEEE	Özkan A,ERDOĞAN A "A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components." , ss.735 - 742, 2011.
ISNAD	Özkan, Aysun - ERDOĞAN, Ayse. "A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components". (2011), 735-742.

APA	Özkan A, ERDOĞAN A (2011). A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkish Journal of Biology, 35(6), 735 - 742.
Chicago	Özkan Aysun,ERDOĞAN Ayse A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkish Journal of Biology 35, no.6 (2011): 735 - 742.
MLA	Özkan Aysun,ERDOĞAN Ayse A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkish Journal of Biology, vol.35, no.6, 2011, ss.735 - 742.
AMA	Özkan A,ERDOĞAN A A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkish Journal of Biology. 2011; 35(6): 735 - 742.
Vancouver	Özkan A,ERDOĞAN A A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components. Turkish Journal of Biology. 2011; 35(6): 735 - 742.
IEEE	Özkan A,ERDOĞAN A "A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components." Turkish Journal of Biology, 35, ss.735 - 742, 2011.
ISNAD	Özkan, Aysun - ERDOĞAN, Ayse. "A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components". Turkish Journal of Biology 35/6 (2011), 735-742.