Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi

CEREN BAYRAÇ (KARAMANOĞLU MEHMETBEY Ü. MÜHENDİSLİK F. BİYOMÜHENDİSLİK B.)
GÜLNUR CAMIZCI ARAN (KARAMANOĞLU MEHMETBEY Ü. FEN BİLİMLERİ ENSTİTÜSÜ)
29 0

Proje Grubu: KBAG Sayfa Sayısı: 89 Proje No: 218Z125 Proje Bitiş Tarihi: 01.02.2023 Metin Dili: Türkçe DOI: 218Z125 İndeks Tarihi: 19-03-2024

Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi

Öz:
Kloramfenikol geniş spektrumlu bir antibiyotik olup hem gram pozitif hem de gram negatif bakterilere karşı etkilidir. Günümüzde sentetik olarak üretilebilmesi, ucuz maliyeti ve geniş spektrum etki mekanizmasına sahip olması nedeniyle insan ve hayvan hastalıklarında sıklıkla kullanılmasına rağmen, gri bebek sendromu, lösemi, aplastik anemi ve kemik iliği baskılanması gibi birçok hastalığa sebep olduğu bilinmektedir. Bu nedenle ülkemizde 2017 yılında yürürlüğü giren yönetmeliklerle kloramfenikol yasaklı madde olarak belirlenmiştir. Aflatoksin M1 Aspergillus cinsi küfler tarafından üretilen, özelikle süt ve süt ürünlerinde bulunan, 4-hidroksi aflatoksin B1`in toksik türevidir. Karsinojen olarak değerlendirilen bu aflatoksin çeşidi pastörizasyon gibi ısıl işlemlere karşı stabilite gösterdiğinden süt ve süt ürünlerinde risk oluşturmaktadır ve bu nedenle 2011 yılında yayınlanan bulaşan yönetmeliğiyle çiğ süt, ısıl işlem görmüş süt ve süt bazlı ürünlerin üretiminde kullanılan sütlerde aflatoksin M1`in maksimum limiti 0.050 ?g/kg olarak belirtilmiştir. Günümüzde kloramfenikol ve aflatoksin M1 için kromatografik ve spektroskobik tayin yöntemleri bulunmasına karşılık ön ekstraksiyon ve saflaştırma basamaklarına ihtiyaç duymaları, maliyetli ve zaman alıcı olmaları nedeniyle farklı yöntemlerin geliştirilmesine ihtiyaç duyulmaktadır. Bu projede, kloramfenikol ve aflatoksin M1`i aynı anda algılayabilecek ve aflatoksin M1 miktar tayini yapabilecek, aptamer bağlayıcılı DNA hidrojel tabanlı tek bir algılama platformunun geliştirilmesi amaçlandı. Bu amaçla, kloramfenikol aptameri ve ona kısmen eşlenik tasarlanan DNA iplik 1 ile DNA hidrojeli sentezlendi ve karakterizasyonu gerçekleştirildi. Sentez esnasında aflatoksin M1 aptameri ve ona kısmen eşlenik tasarlanan DNA iplik 2 de polimer yapıya entegre edildi. Her iki ayrı hedef için tasarlanan tespit yöntemine ait optimizasyon çalışmaları yapılarak en uygun sentez, entegrasyon ve tespit koşulları belirlendi. Algılama platformuna ait performans parametreleri olan tespit (LOD) ve tayin limiti (LOQ) kloramfenikol için 0.10 nM LOD, aflatoksin M1 için ise 1.68 nM LOD ve 5.16 nM LOQ olarak belirlendi. Sentezlenen DNA hidrojelin hedef moleküllere karşı verdiği cevap hem solüsyon ortamında hem de gerçek örnek olarak seçilen süt örneğinde denendi ve iki hedefe karşı tasarlanan algılama platformunun çalışır olduğu gösterildi. Sonuç olarak; proje başarılı bir şekilde tamamlandı.
Anahtar Kelime: Aptamer DNA Hidrojel Kloramfenikol Aflatoksin M1 Dual Aptasensör

Development of DNA hydrogel-based dual aptasensor platform specific for chloramphenicol and aflatoxin M1 food contaminants

Öz:
Chloramphenicol is a broad-spectrum antibiotic and it is effective against both gram-positive and gram-negative bacteria. Although it has been frequently used in human and animal diseases due to its synthetic production, cheap cost and broad spectrum mechanism of action, it may cause many diseases such as gray baby syndrome, leukemia, aplastic anemia and bone marrow suppression. For this reason, chloramphenicol has been determined as a prohibited substance in our country with the regulation adopted in 2017. Aflatoxin M1 is a toxic metabolite of 4-hydroxy aflatoxin B1,produced by molds of the genus Aspergillus and found in milk and dairy products. This type of aflatoxin, which is considered a carcinogen, poses a risk in milk and dairy products since it shows stability against heat treatments such as pasteurization. Therefore, with the contaminant regulation published in 2011, the maximum limit of aflatoxin M1 in pasteurized milk and its product is specified as 0.050 ?g/kg. Although there have been certain chromatographic and spectrosphotometric determination methods for chloramphenicol and aflatoxin M1, different detection methods are needed since these methods require pre-extraction and purification steps, are costly and time consuming. In this project, it was aimed to develop a single detection platform based on aptamer-functionalized DNA hydrogel that can simultaneously detect chloramphenicol and aflatoxin M1 and also quantify aflatoxin M1. For this purpose, DNA hydrogel was synthesized with the use of chloramphenicol aptamer and partially conjugated DNA strand 1 and it was characterized. During the synthesis, the aflatoxin M1 aptamer and the partially conjugated DNA strand 2 were integrated into the polymer structure. Optimization studies were carried out for the detection method designed for both targets, and the most suitable synthesis, integration and detection conditions were determined. The performance parameters of the detection platform, limit of detection (LOD) and limit of quantification (LOQ) were determined as 0.10 nM LOD for chloramphenicol, 1.68 nM LOD and 5.16 nM LOQ for aflatoxin M1, respectively. The response of the synthesized DNA hydrogel to the target molecules was tested both in the solution medium and in the milk sample selected as the real sample, and it was shown that the detection platform designed against the two targets works properly. In conclusion; the project was successfully completed.
Anahtar Kelime: Aptamer DNA Hidrojel Kloramfenikol Aflatoksin M1 Dual Aptasensör

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APA BAYRAC C, ARAN G (2023). Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. , 0 - 89. 218Z125
Chicago BAYRAC CEREN,ARAN GÜLNUR CAMIZCI Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. (2023): 0 - 89. 218Z125
MLA BAYRAC CEREN,ARAN GÜLNUR CAMIZCI Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. , 2023, ss.0 - 89. 218Z125
AMA BAYRAC C,ARAN G Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. . 2023; 0 - 89. 218Z125
Vancouver BAYRAC C,ARAN G Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. . 2023; 0 - 89. 218Z125
IEEE BAYRAC C,ARAN G "Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi." , ss.0 - 89, 2023. 218Z125
ISNAD BAYRAC, CEREN - ARAN, GÜLNUR CAMIZCI. "Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi". (2023), 0-89. https://doi.org/218Z125
APA BAYRAC C, ARAN G (2023). Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. , 0 - 89. 218Z125
Chicago BAYRAC CEREN,ARAN GÜLNUR CAMIZCI Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. (2023): 0 - 89. 218Z125
MLA BAYRAC CEREN,ARAN GÜLNUR CAMIZCI Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. , 2023, ss.0 - 89. 218Z125
AMA BAYRAC C,ARAN G Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. . 2023; 0 - 89. 218Z125
Vancouver BAYRAC C,ARAN G Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi. . 2023; 0 - 89. 218Z125
IEEE BAYRAC C,ARAN G "Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi." , ss.0 - 89, 2023. 218Z125
ISNAD BAYRAC, CEREN - ARAN, GÜLNUR CAMIZCI. "Kloramfenikol ve Aflatoksin M1 Gıda Bulaşanlarına Özgü DNA Hidrojel Tabanlı Dual Aptasensör Platformunun Geliştirilmesi". (2023), 0-89. https://doi.org/218Z125
Sayfa Oluşturulma Tarihi
24-04-2024 10:58

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