Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia

GEDİKOĞLU, Gündüz; YALMAN, Nevin; DEVECİOĞLU, Ömer; AĞAOĞLU, Leyla; ERYILMAZ, Ertuğrul; ÖZBEK, Uğur; Anak, Seniye Sema; OZGEN, ÜNSAL; Sarper, Nazan

Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia

Ünsal ÖZGEN, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Pediatrik Hematoloji Bilim Dalı, İstanbul, Türkiye)

Sema ANAK, (Girilmedi)

Uğur ÖZBEK, (Girilmedi)

Nazan SARPER, (Girilmedi)

Ertuğrul ERYILMAZ, (Girilmedi)

Leyla AĞAOĞLU, (Girilmedi)

Ömer DEVECİOĞLU, (Girilmedi)

Nevin YALMAN, (Girilmedi)

Gündüz GEDİKOĞLU (Girilmedi)

Turkish Journal of Hematology

1 0

Yıl: 2000 Cilt: 17 Sayı: 4 Sayfa Aralığı: 183 - 188 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia

Öz:

Wilms tumor 1 (WT1) gene is a tumor supressor gene, expressed in malignant and normal hematopoietic progenitor cells. Prognostic significance of this gene in childhood acute lymphoid leukemia (ALL) is not clear. We evaluated the presence of WT1 expression in bone marrow samples of 28 children with de novo ALL at diagnosis by two step RT-PCR. Expression of WT1 gene was detected in 78.5% of patients. There was no correlation between WT1 gene expression and age, sex, FAB type, leukocyte count, and presence of t(4;11) and t(9;22). All patients were treated with modified BFM 86 protocol. There was no difference in the complete remission (CR) rate between WT1 positive and negative patients. Event free survival (EFS) and overall survival (OS) of WT1 positive and negative patients were also not significant. We conclude that expression of WT1 gene is not associated with specific characteristics of ALL blast cells and is not a prognostic factor for CR, remission duration and overall survival.

Anahtar Kelime: Child Leukemia, Lymphocytic, Acute Genes, Wilms Tumor Stem Cells Gene Expression Prognosis

Konular: Hematoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Reddy JC, Hosono S, Licht JD. The transcriptional effect of WT1 is modulated by choice of expression vector. J Biol Chem 1995;270:29976-82.
2. Miyagi T, Ajuha H, Kubota T, Kubonishi I, Koeffler HP, Miyoshi I. Expression of the Wilms tumor 1 gene in human leukemia cells. Leukemia 1993;7:970- 7.
3. Brenner B, Wildhardt G, Schneider S, Royer-Pokora B. RNA polymerase chain reaction detects different levels of four alternatively spliced WT1 transcripts in Wilm’s tumors. Oncogene 1992;7:1431-3.
4. Inoue K, Sugiyama H, Ogawa H, Nakagawa M, Yamagami T, Miwa H et al. WT1 as a new prognostic factor and a new marker for the detection of minimal residual disease in acute leukemia. Blood 1994;84:3071-9.
5. Hartley AL, Birch JM, Harris M, Blair V, Morris-Jones PH, Gattamanen HR et al. Leukemia, lymphoma and related disorders in families of children diagnosed with Wilms tumor. Cancer Genet Cytoge net 1994;77:129-33.
6. Schmid D, Heinze G, Linnerth B, Tisljat K, Kusec R, Geissler K et al. Prognostic significance of WT1 gene expression at diagnosis in adult de novo acute myeloid leukemia. Leukemia 1997;11:639-43.
7. Fraizer GC, Patmasirivat P, Zhang X, Saunders GF. Expression of the tumor supressor gene WT1 in both human and mouse bone marrow. Blood 1995;86:4704-6.
8. Chomczynski P, Sacchi N. Single step method of RNA isolation by acid guanidum thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162: 156-9.
9. Becker S, Quay J, Saukup J. Cytokine (tumor necrosis factor, IL-6 and IL-8) production by respiratory syncytial virus-infected human alveolar macrophages. J Immunol 1991;147:4307-12.
10. Rubnitz JE, Behm FG, Downing JR. 11q23 rearrengements in acute leukemia. Leukemia 1996;10:74- 82.
11. Frenoy N, Chabli A, Sol O, Golschmit E, Lemonnier MP,Misset JL, Debiure B. Application of a new protocol for nested PCR to the detection of minimal residual bcr/abl transcripts. Leukemia 1994;8: 1411-4.
12. Miwa H, Beran M, Saunders GF. Expression of the Wilms’ tumor gene (WT1) in human leukemias. Leukemia 1992;6:405-9.
13. Brieger J, Weidmann E, Fenchel K, Mitrou PS, Hoelzer D, Bergmann L. The expression of the Wilms’ tumor gene in acute myelocytic leukemias as a possible marker for leukemic blast cells. Leukemia 1994;8:2138-43.
14. Menssen HD, Renkl HJ, Maurer J, Notter M, Schwartz S, Reinhardt R, Thiel E. Presence of the Wilms’ tumor gene (wt1) transcripts and the WT1 nuclear protein in the majority of human acute leukemias. Leukemia 1995;9:1060-
15. Sekiya M, Adachi M, Hinoda Y, Imai K, yachi A. Downregulation of Wilms’ tumor gene (wt1) during myelomonocytic differantiation in HL60 cells. Blood 1994;83:1876-82.
16. Maurer U, Weidmann E, Karakas T, Hoelzer D, Bergmann L. Wilms’ tumor gene (wt1) mRNA equally expressed in blast cells from acute myeloid leukemia and normal CD34+ progenitors. Blood 1997;90:4230-8.
17. Neigemann E, Wehner S, Kornhuber B, Schwabe D, Ebener U. WT1 gene expression in childhood le ukemias. Acta Haematol 1999;102:72-6.
18. Gaiger A, Linnerth B, mann G, Schmid D, Heinze G, Tisljar K, Haas OA, Gadner H. Wilms’ tumour gene (wt1) expression at diagnosis has no prognostic relevance in childhood acute lymphoblastic leukemia treated by an intensive chemotherapy protocol. Eur J Haematol 1999;63:86-93.
19. Bergmann L, Miething C, Maurer U, Brieger J, Karakas T, Weidmann E, Hoelzer D. High levels of Wilms tumor gene (wt1) mRNA in acute myeloid leukemia are associated with a worse long term outcome. Blood 1997;90:1217-25.

APA	OZGEN Ü, Anak S, ÖZBEK U, Sarper N, ERYILMAZ E, AĞAOĞLU L, DEVECİOĞLU Ö, YALMAN N, GEDİKOĞLU G (2000). Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. , 183 - 188.
Chicago	OZGEN ÜNSAL,Anak Seniye Sema,ÖZBEK Uğur,Sarper Nazan,ERYILMAZ Ertuğrul,AĞAOĞLU Leyla,DEVECİOĞLU Ömer,YALMAN Nevin,GEDİKOĞLU Gündüz Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. (2000): 183 - 188.
MLA	OZGEN ÜNSAL,Anak Seniye Sema,ÖZBEK Uğur,Sarper Nazan,ERYILMAZ Ertuğrul,AĞAOĞLU Leyla,DEVECİOĞLU Ömer,YALMAN Nevin,GEDİKOĞLU Gündüz Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. , 2000, ss.183 - 188.
AMA	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. . 2000; 183 - 188.
Vancouver	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. . 2000; 183 - 188.
IEEE	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G "Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia." , ss.183 - 188, 2000.
ISNAD	OZGEN, ÜNSAL vd. "Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia". (2000), 183-188.

APA	OZGEN Ü, Anak S, ÖZBEK U, Sarper N, ERYILMAZ E, AĞAOĞLU L, DEVECİOĞLU Ö, YALMAN N, GEDİKOĞLU G (2000). Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. Turkish Journal of Hematology, 17(4), 183 - 188.
Chicago	OZGEN ÜNSAL,Anak Seniye Sema,ÖZBEK Uğur,Sarper Nazan,ERYILMAZ Ertuğrul,AĞAOĞLU Leyla,DEVECİOĞLU Ömer,YALMAN Nevin,GEDİKOĞLU Gündüz Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. Turkish Journal of Hematology 17, no.4 (2000): 183 - 188.
MLA	OZGEN ÜNSAL,Anak Seniye Sema,ÖZBEK Uğur,Sarper Nazan,ERYILMAZ Ertuğrul,AĞAOĞLU Leyla,DEVECİOĞLU Ömer,YALMAN Nevin,GEDİKOĞLU Gündüz Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. Turkish Journal of Hematology, vol.17, no.4, 2000, ss.183 - 188.
AMA	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. Turkish Journal of Hematology. 2000; 17(4): 183 - 188.
Vancouver	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia. Turkish Journal of Hematology. 2000; 17(4): 183 - 188.
IEEE	OZGEN Ü,Anak S,ÖZBEK U,Sarper N,ERYILMAZ E,AĞAOĞLU L,DEVECİOĞLU Ö,YALMAN N,GEDİKOĞLU G "Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia." Turkish Journal of Hematology, 17, ss.183 - 188, 2000.
ISNAD	OZGEN, ÜNSAL vd. "Prognostic significance of Wilms tumor 1 gene in childhood acute lymphoblastic leukemia". Turkish Journal of Hematology 17/4 (2000), 183-188.