Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features

YALMAN, Nevin; DEVECİOĞLU, Ömer; AĞAOĞLU, Leyla; Kandilci, Ayten; ÖZBEK, Uğur; OZGEN, ÜNSAL; SIRMA, Sema; GEDİKOĞLU, Gündüz; Sarper, Nazan; ERYILMAZ, Ertuğrul; Anak, Seniye Sema

Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features

Turkish Journal of Hematology

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Yıl: 2000 Cilt: 17 Sayı: 4 Sayfa Aralığı: 197 - 206 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features

Öz:

BCR/ABL expression, which is the molecular equivalent of the Philadelphia chromosome, is an independent poor risk factor in acute lymphoblastic leukemia (ALL). We used a two-step (nested) reverse transcriptase polimerase chain reaction (RT-PCR) assay to examine BCR/ABL expression in the diagnostic bone marrow specimen of children with ALL, prospectively. Among 75 de novo ALL patients, 4 (%5.3) were found to be BCR/ABL-positive, whereas 4 of 17 relapsed patients (23.5%) were positive.This preliminary study in Turkish children showed an incidence similar to reports from Europe and the U.S.A. More intensive chemotherapies and allogeneic bone marrow transplantations (BMT) during the first remission were planned if a donor was available. Out of 8 BCR/ABL-positive patients, complete remission (CR) was achieved in 7 patients and partial remission (PR) was achieved in 1 patient. Three patients underwent allogeneic BMT during the first CR and 1 under went autologous BMT during the first PR. The Kaplan-Meier estimate o f event-free survival (EFS) of BCR/ABL negative de novo ALL patients was 78.36% at 3 years, whereas the EFS of positive patients was 31.25% at 26 ± 6.4 months. Molecular screening for the Philadelphia chromosome should become a part of the routine diagnostic panel in ALL patients in order to predict which patients have a poor prognosis and need tailored therapy.

Anahtar Kelime: Leukemia, Lymphocytic, Acute Reverse Transcriptase Polymerase Chain Reaction Prospective Studies Diagnostic Techniques and Procedures Philadelphia Chromosome

Konular: Hematoloji

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

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APA	Sarper N, ÖZBEK U, AĞAOĞLU L, OZGEN Ü, Kandilci A, SIRMA S, Anak S, YALMAN N, ERYILMAZ E, DEVECİOĞLU Ö, GEDİKOĞLU G (2000). Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. , 197 - 206.
Chicago	Sarper Nazan,ÖZBEK Uğur,AĞAOĞLU Leyla,OZGEN ÜNSAL,Kandilci Ayten,SIRMA Sema,Anak Seniye Sema,YALMAN Nevin,ERYILMAZ Ertuğrul,DEVECİOĞLU Ömer,GEDİKOĞLU Gündüz Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. (2000): 197 - 206.
MLA	Sarper Nazan,ÖZBEK Uğur,AĞAOĞLU Leyla,OZGEN ÜNSAL,Kandilci Ayten,SIRMA Sema,Anak Seniye Sema,YALMAN Nevin,ERYILMAZ Ertuğrul,DEVECİOĞLU Ömer,GEDİKOĞLU Gündüz Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. , 2000, ss.197 - 206.
AMA	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. . 2000; 197 - 206.
Vancouver	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. . 2000; 197 - 206.
IEEE	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G "Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features." , ss.197 - 206, 2000.
ISNAD	Sarper, Nazan vd. "Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features". (2000), 197-206.

APA	Sarper N, ÖZBEK U, AĞAOĞLU L, OZGEN Ü, Kandilci A, SIRMA S, Anak S, YALMAN N, ERYILMAZ E, DEVECİOĞLU Ö, GEDİKOĞLU G (2000). Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. Turkish Journal of Hematology, 17(4), 197 - 206.
Chicago	Sarper Nazan,ÖZBEK Uğur,AĞAOĞLU Leyla,OZGEN ÜNSAL,Kandilci Ayten,SIRMA Sema,Anak Seniye Sema,YALMAN Nevin,ERYILMAZ Ertuğrul,DEVECİOĞLU Ömer,GEDİKOĞLU Gündüz Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. Turkish Journal of Hematology 17, no.4 (2000): 197 - 206.
MLA	Sarper Nazan,ÖZBEK Uğur,AĞAOĞLU Leyla,OZGEN ÜNSAL,Kandilci Ayten,SIRMA Sema,Anak Seniye Sema,YALMAN Nevin,ERYILMAZ Ertuğrul,DEVECİOĞLU Ömer,GEDİKOĞLU Gündüz Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. Turkish Journal of Hematology, vol.17, no.4, 2000, ss.197 - 206.
AMA	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. Turkish Journal of Hematology. 2000; 17(4): 197 - 206.
Vancouver	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features. Turkish Journal of Hematology. 2000; 17(4): 197 - 206.
IEEE	Sarper N,ÖZBEK U,AĞAOĞLU L,OZGEN Ü,Kandilci A,SIRMA S,Anak S,YALMAN N,ERYILMAZ E,DEVECİOĞLU Ö,GEDİKOĞLU G "Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features." Turkish Journal of Hematology, 17, ss.197 - 206, 2000.
ISNAD	Sarper, Nazan vd. "Detection of BCR / ABL transcripts by reverse transcriptase polimerase chain reaction in pediatric acute lymphoblastic leukemia: Incidence and clinical features". Turkish Journal of Hematology 17/4 (2000), 197-206.