Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats

Silici, Sibel; ARSLAN, SOLEY; KOC, A. Nedret; Perçin Renders, Duygu; ER, OZGUR

Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats

Soley ARSLAN, (Erciyes Üniversitesi, Diş Hekimliği Fakültesi, Restoratif Diş Hastalıkları ve Tedavisi Anabilim Dalı, Kayseri, Türkiye)

Sibel SİLİCİ, (Erciyes Üniversitesi, Safiye Çıkrıkoğlu Meslek Yüksek Okulu, Kayseri, Türkiye)

DUYGU PERÇİN RENDERS, (Erciyes Üniversitesi, Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, Kayseri, Türkiye)

Ayşe Nedret KOÇ, (Erciyes Üniversitesi, Tıp Fakültesi, Mikrobiyoloji Anabilim Dalı, Kayseri, Türkiye)

Özgür ER (Erciyes Üniversitesi, Diş Hekimliği Fakültesi, Restoratif Diş Hastalıkları ve Tedavisi Anabilim Dalı, Kayseri, Türkiye)

Turkish Journal of Biology

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Yıl: 2012 Cilt: 36 Sayı: 1 Sayfa Aralığı: 65 - 73 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats

Öz:

Bu çalışmada klorhekzidin ve propolis ekstraktlarının mutans streptokoklar ve ratlarda çürük gelişimi üzerine etkisi değerlendirildi. Propolis ekstraktlarının (etanol, metanol, kloroform, hekzan, propilen glikol ve etil asetat) Streptococcus mutans UA159 and Streptococcus sobrinus 6715 üzerine antimikrobiyal etkisi çalışıldı. MIC ve MBC, Klinik Laboratuarlar için Uluslararası Komiteye göre broth makrodilüsyonla belirlendi. Hayvan çalışmasında, ratlar S. sobrinus ile enfekte edildi ve kariyojenik diyetle beslendi. Ratlara beş hafta süresince propolis ekstrakları (hekzan, etanol) günde iki kez topikal olarak uygulandı. Deneysel periyottan sonra, dental plağın mikrobiyal kompozisyonu ve çürük skorları belirlendi. Propolisin hekzan (HEP) ve etanol (EEP) ekstraktları test edilen mikroorganizmalara karşı yüksek antimikrobial aktivite gösterdiler. HEP ve EEP nin kimyasal kompozisyonu gaz kromatografi /kütle spektrometri ile belirlendi. Her iki ekstrakta pinbanksin ve naringenin ana fenolik bileşenlerdi. Ekstraktların her ikisi düz yüzey çürük şiddetini önemli oranda azalttı. Sulkal çürük şiddeti (Ds) kontrol grubu ile karşılaştırıldığında EEP ile azaldı (P < 0,05). Sonuç olarak, poplar propolis mutans streptokoklara karşı antimikrobiyal aktiviteye sahiptir ve çürüğün önlenmesinde kullanılabilir.

Anahtar Kelime: kavak antimikrobiyal özellikler propolis özüt sıçan diş çürüğü Streptococcus mutans Streptococcus sobrinus

Konular: Biyoloji

Kavak propolisinin mutans streptokoklar üzerine antimikrobiyal aktivitesi ve ratlarda çürük gelişimine etkisi

Öz:

The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts (including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate) on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract of propolis (EEP) and hexane extract of propolis (HEP) for 5 weeks. After the experimental period, both the microbial composition of their dental plaque and their caries score were assessed. EEP and HEP showed the highest antimicrobial activity against the tested microorganisms. The chemical composition of EEP and HEP were examined using gas chromatography-mass spectrometry. The pinobanksin and naringenin were the main phenolic compounds identified in both extracts. Further, both of the extracts significantly reduced the severity of smooth surface caries in vivo. The severity of the sulcal caries (Ds) was significantly reduced by EEP compared to the control group (P < 0.05). In conclusion, poplar propolis demonstrated antimicrobial activity against mutans streptococci, indicating that it may be used in caries prevention.

Anahtar Kelime: propolis extracts rats dental caries Streptococcus mutans Streptococcus sobrinus poplars antimicrobial properties

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ARSLAN S, Silici S, Perçin Renders D, KOC A, ER O (2012). Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. , 65 - 73.
Chicago	ARSLAN SOLEY,Silici Sibel,Perçin Renders Duygu,KOC A. Nedret,ER OZGUR Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. (2012): 65 - 73.
MLA	ARSLAN SOLEY,Silici Sibel,Perçin Renders Duygu,KOC A. Nedret,ER OZGUR Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. , 2012, ss.65 - 73.
AMA	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. . 2012; 65 - 73.
Vancouver	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. . 2012; 65 - 73.
IEEE	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O "Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats." , ss.65 - 73, 2012.
ISNAD	ARSLAN, SOLEY vd. "Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats". (2012), 65-73.

APA	ARSLAN S, Silici S, Perçin Renders D, KOC A, ER O (2012). Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turkish Journal of Biology, 36(1), 65 - 73.
Chicago	ARSLAN SOLEY,Silici Sibel,Perçin Renders Duygu,KOC A. Nedret,ER OZGUR Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turkish Journal of Biology 36, no.1 (2012): 65 - 73.
MLA	ARSLAN SOLEY,Silici Sibel,Perçin Renders Duygu,KOC A. Nedret,ER OZGUR Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turkish Journal of Biology, vol.36, no.1, 2012, ss.65 - 73.
AMA	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turkish Journal of Biology. 2012; 36(1): 65 - 73.
Vancouver	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats. Turkish Journal of Biology. 2012; 36(1): 65 - 73.
IEEE	ARSLAN S,Silici S,Perçin Renders D,KOC A,ER O "Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats." Turkish Journal of Biology, 36, ss.65 - 73, 2012.
ISNAD	ARSLAN, SOLEY vd. "Antimicrobial activity of poplar propolis on mutans streptococci and caries development in rats". Turkish Journal of Biology 36/1 (2012), 65-73.