Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Soylu, Elif; GONÜLOL, ARIF

Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Elif Neyran SOYLU, (Giresun Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Giresun, Türkiye)

Arif GÖNÜLOL (Ondokuz Mayıs Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Samsun, Türkiye)

Turkish Journal of Biology

7 0

Yıl: 2012 Cilt: 36 Sayı: 3 Sayfa Aralığı: 247 - 254 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Öz:

Günümüzde moleküler teknikler pek çok alanda kullanılmakta ve yapılan çalışmaların güvenilirliğini artırmaktadır. Araştırmanın genel amacı, küresel yeşil alglerin klasik morfolojik karakterlerini arazi ve kültür şartları altında modern moleküler analizleri kullanarak karşılaştırmaktır. Bu amacı gerçekleştirebilmek için öncelikle Liman ve Cernek göllerinden (Samsun, Türkiye) alınan küresel yeşil alglerin farklı klon kültürlerinin izolasyonu gerçekleştirilmiştir. Kültür şartları altında yeşil alglerin morfolojik çeşitliliği gözlemlenerek, bu suşların 18S rRNA gen dizilerine göre filogenetik ilişkileri belirlenmiştir. DNA dizi analizine göre 2 izolatın Scenedesmus subspicatus (98%) ve Desmodesmus sp. (100%) türlerine çok benzeklik gösterdikleri tespit edilmiştir.

Anahtar Kelime: göller Scenedesmus DNA dizi analizi yeşil algler Türkiye moleküler genetik ribozomal RNA morfoloji Karadeniz Bölgesi tanımlama izolasyon Samsun Desmodesmus

Konular: Biyoloji

Kızılırmak Deltası’ndaki göllerden izole edilen küresel yeşil alglerin morfolojik ve 18S rRNA analizi

Öz:

Molecular techniques are used today in many areas and have increased the confi dence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus (98%) and Desmodesmus sp. (100%).

Anahtar Kelime: green algae Turkey molecular genetics ribosomal RNA morphology Black Sea Region descriptions isolation Samsun Desmodesmus lakes Scenedesmus DNA sequencing

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Soylu E, GONÜLOL A (2012). Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. , 247 - 254.
Chicago	Soylu Elif,GONÜLOL ARIF Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. (2012): 247 - 254.
MLA	Soylu Elif,GONÜLOL ARIF Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. , 2012, ss.247 - 254.
AMA	Soylu E,GONÜLOL A Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. . 2012; 247 - 254.
Vancouver	Soylu E,GONÜLOL A Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. . 2012; 247 - 254.
IEEE	Soylu E,GONÜLOL A "Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta." , ss.247 - 254, 2012.
ISNAD	Soylu, Elif - GONÜLOL, ARIF. "Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta". (2012), 247-254.

APA	Soylu E, GONÜLOL A (2012). Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology, 36(3), 247 - 254.
Chicago	Soylu Elif,GONÜLOL ARIF Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology 36, no.3 (2012): 247 - 254.
MLA	Soylu Elif,GONÜLOL ARIF Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology, vol.36, no.3, 2012, ss.247 - 254.
AMA	Soylu E,GONÜLOL A Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology. 2012; 36(3): 247 - 254.
Vancouver	Soylu E,GONÜLOL A Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta. Turkish Journal of Biology. 2012; 36(3): 247 - 254.
IEEE	Soylu E,GONÜLOL A "Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta." Turkish Journal of Biology, 36, ss.247 - 254, 2012.
ISNAD	Soylu, Elif - GONÜLOL, ARIF. "Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta". Turkish Journal of Biology 36/3 (2012), 247-254.