Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants

ÜSTÜN, Ayşen Sülün; KOÇ, ESRA

Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants

Esra KOÇ, (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Ayşen Sülün ÜSTÜN (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Turkish Journal of Biology

1 0

Yıl: 2012 Cilt: 36 Sayı: 3 Sayfa Aralığı: 357 - 371 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants

Öz:

Bu araştırmada üç biber çeşidinde P. capsici’nin farklı inokulum konsantrasyonlarının (102, 103 ve 104 zoospor $mL^ {-1}$) belirli zamanlarda (2., 4. ve 6. günlerde) oluşturacağı enfeksiyon şiddeti, meydana gelecek olan enfeksiyonun dayanıklı ve duyarlı biber fidelerinde peroksidaz (POX), katalaz (CAT) ve fenolikler üzerindeki etkisi ile patojene karşı dayanıklılığı sağlayan savunma mekanizması açıklanmaya çalışılmıştır. Çalışmada PM-702’nin kullandığımız izolata karşı dayanıklılığı yüksek bulunurken, KM-Hot ve DEM-8 çeşitlerinin izolata karşı duyarlı reaksiyon verdiği belirlenmiştir. Üç biber çeşidinin yaprak ve gövdelerinde yapılan analizler sonucunda kök çürüklüğü patojeni P. capsici ile inokulasyona karşı dirençli ve duyarlı biber çeşitleri arasında biyokimyasal parametrelerde önemli değişimler gözlenmiştir. POX ve CAT’ın maksimum artışı dirençli PM-702 çeşidinde gözlenmiştir. Buna rağmen yüksek inokulum konsantrasyonunda (104 zoospor $mL^ {-1}$), özellikle duyarlı çeşitlerde CAT’da bir aktivite kaybı belirlenmiştir. Fenoliklerde maksimum artış enfeksiyonu takiben 6. günde PM-702’nin gövdesinde ve DEM-8’in yapraklarında görülmüştür. Sonuçlar Phytophthora kök çürüklüğü gelişimi süresince, P. capsici ile antioksidan sistem arasında bir bağlantı olduğunu göstermiştir.

Anahtar Kelime: peroksidaz fenolik bileşikler kök çürükçülleri bitki hastalıkları etkileşim bitki bozuklukları mantar bitki patojen mantarlar Phytophthora capsici konukçu konuk ilişkisi Capsicum annuum dayanıklı çeşitler katalaz nekroz

Konular: Biyoloji

Phytophthora capsici L. inokulasyonunun dirençli ve duyarlı biber (Capsicum annuum L.) bitkilerinin hastalık şiddeti, nekroz uzunluğu, peroksidaz ve katalaz aktivitesi, fenolik içeriği üzerine etkisi

Öz:

This study explored the level of infection caused by different inoculum concentrations (102, 103, and 104 zoospores $mL^ {-1}$) of Phytophthora capsici in 3 pepper cultivars at days 2, 4, and 6. The effect that the infection induced on the peroxidase (POX), catalase (CAT), and phenolics of resistant and sensitive seedlings, as well as the defense mechanism against the pathogen, were also investigated. The resistance of PM-702 against the isolate used was high, whereas KM-Hot and DEM-8 displayed sensitive reactions. As a result of analyses of the leaves and stems from the 3 pepper cultivars, important changes in biochemical parameters were observed between resistant and sensitive cultivars after inoculation with the root rot pathogen P. capsici. The maximum increase of POX and CAT was observed in the resistant PM-702 cultivar. However, with a high inoculum concentration (104 zoospores $mL^ {-1}$), a loss of CAT activity was determined, especially in susceptible cultivars. The maximum increase in phenolics was detected in leaves of susceptible DEM-8 and stems of PM-702 on day 6 following infection. The results suggest that during Phytophthora root rot development there is a relation between the disease induced by P. capsici and the antioxidant system.

Anahtar Kelime: Phytophthora capsici host guest relations Capsicum annuum resistant cultivars catalase necroses peroxidase phenolic compounds root rots plant diseases interactions plant disorders fungi plant pathogenic fungi

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KOÇ E, ÜSTÜN A (2012). Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. , 357 - 371.
Chicago	KOÇ ESRA,ÜSTÜN Ayşen Sülün Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. (2012): 357 - 371.
MLA	KOÇ ESRA,ÜSTÜN Ayşen Sülün Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. , 2012, ss.357 - 371.
AMA	KOÇ E,ÜSTÜN A Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. . 2012; 357 - 371.
Vancouver	KOÇ E,ÜSTÜN A Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. . 2012; 357 - 371.
IEEE	KOÇ E,ÜSTÜN A "Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants." , ss.357 - 371, 2012.
ISNAD	KOÇ, ESRA - ÜSTÜN, Ayşen Sülün. "Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants". (2012), 357-371.

APA	KOÇ E, ÜSTÜN A (2012). Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turkish Journal of Biology, 36(3), 357 - 371.
Chicago	KOÇ ESRA,ÜSTÜN Ayşen Sülün Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turkish Journal of Biology 36, no.3 (2012): 357 - 371.
MLA	KOÇ ESRA,ÜSTÜN Ayşen Sülün Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turkish Journal of Biology, vol.36, no.3, 2012, ss.357 - 371.
AMA	KOÇ E,ÜSTÜN A Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turkish Journal of Biology. 2012; 36(3): 357 - 371.
Vancouver	KOÇ E,ÜSTÜN A Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turkish Journal of Biology. 2012; 36(3): 357 - 371.
IEEE	KOÇ E,ÜSTÜN A "Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants." Turkish Journal of Biology, 36, ss.357 - 371, 2012.
ISNAD	KOÇ, ESRA - ÜSTÜN, Ayşen Sülün. "Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants". Turkish Journal of Biology 36/3 (2012), 357-371.