Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?

kaya, semra; SOMI SCHAFER, Sabine; Aslan, Selim; KACAR, Cihan; KURU, Mushap; Kaya, Duygu

Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?

Duygu KAYA, (Kafkas Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kars, Türkiye)

Selim ASLAN, (Ankara Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Ankara, Türkiye)

Semra KAYA, (Kafkas Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kars, Türkiye)

Mushap KURU, (Kafkas Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kars, Türkiye)

Cihan KAÇAR, (Kafkas Üniversitesi, Veteriner Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, Kars, Türkiye)

Sabine SOMI SCHAFER (Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, A 1210, Vienna, Austria)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2013 Cilt: 19 Sayı: 2 Sayfa Aralığı: 299 - 304 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?

Öz:

Uzun etkili GnRH agonistleri köpeklerde hem östrusun uyarılmasında hem de baskılanmasında kullanılmaktadırlar. Bu çalışmada, pubertas öncesi dönemdeki dişi köpeklerde deslorelin implant (4.7 ve 9.4 mg) kullanımının “flare-up” dönemi olarak değerlendirilebilecek klinik ve endokrinolojik kısa dönem etkilerinin varlığı araştırılmıştır. Bu amaçla pubertas öncesi dönemde olduğu belirlenen ortalama 4 aylık yaşta on dört adet sağlıklı melez dişi kopek kullanıldı. Köpeklere deri altı yolla 9.4 mg (G1, n=5) ve 4.7 mg (G2, n=4) deslorelin (Suprelorin®) etken maddesi içeren implantlar ya da plasebo (sodyum klorür %0.9; G3; n=5) uygulandı. Östrus belirtileri günlük olarak fiziksel değişiklikler ve davranış değişiklikleri yönünden takip edildi. Ayrıca çalışma süresince vaginal sitoloji, serum progesteron (P4) ve östradiol 17β (E2) konsantrasyonları haftalık olarak izlendi. Tedavi (G1 ve G2) ve kontrol grubundaki (G3) hayvanların hiçbirinde östrus bulgusu belirlenmedi. İmplantın yerleştirildiği bölgede herhangi bir lokal yan etki gözlenmedi. Grup 1’de iki köpekte (2/5; %40) serum P4 düzeyinde bir artış gözlenirken, bu köpeklerden yalnızca birinde (P4=6,37 ng/ml) serum E2 düzeyinin 37 pg/ml’ye kadar yükseldiği belirlendi. Grup 2’de 13 haftalık tedavi süresince serum P4 ve E2 düzeylerinde önemli bir değişiklik gözlenmedi. Bu çalışmadan elde edilen bulgular, pubertas öncesi köpeklerde deslorelin implantın, yetişkin köpeklerde implante edildikten sonraki ilk bir ay içerisinde sıklıkla gözlenen “flare-up etkisine” neden olmadığını göstermiştir. Bununla birlikte deslorelin implant uygulamalarının özellikle pubertasa yakın dönemlerde oluşturabileceği “flare-up” etkisine dair yeni çalışmalara ihtiyaç vardır.

Anahtar Kelime: progesteron kızgınlık dönemi kızgınlık dişi köpek östradiol sodyum klorür üreme davranışı anöstrüs kan serumu GnRH vajina sitoloji köpek agonistler cinsel davranış hayvan davranışları içeyerleşim endokrin sistem

Konular: Veterinerlik

Prepubertal köpeklerde GnRH analoğu deslorelinin kısa dönem klinik ve endokrinolojik etkileri: “Flare-up” gözlenir mi?

Öz:

Long-acting GnRH agonists have been used both in canine estrus induction and prevention. The objective of the study was to investigate the clinical and endocrine short-term effects of a deslorelin implant (4.7 and 9.4 mg) in terms of initial “flare-up” in prepubertal bitches. Fourteen healthy, cross-breed prepubertal female dogs, aged 4 months, were used in the study. An implant containing 9.4 mg (G1, n=5) and 4.7 mg (G2, n=4) deslorelin (Suprelorin®) or a placebo (sodium chloride 0.9%, G3, n=5) was inserted subcutaneously. Estrus was monitored once daily by physical and sexual behavioral changes. Vaginal cytology, serum progesterone (P4) and estradiol 17β (E2) concentrations were monitored weekly for the first five weeks throughout the treatment. None of the bitches in the treatment (G1 and G2) and control (G3) group showed any signs of estrous throughout the study period. No local reactions were observed at the implantation site. Two bitches showed an increase in serum P4 concentrations (>1.0 ng/ml) in G1 (2/5; 40%), however, only one of these two animals (P4=6.37 ng/ml) showed an increase in serum E2 concentrations up to 37 pg/ml. No marked increase in serum P4 and E2 concentrations were observed during the first 13 weeks of treatment in G2. These data demonstrate that in prepubertal bitches, insertion of a deslorelin implant does not cause a “flare-up effect” which commonly occurs in anestrus adult bitches during the first month after implantation. However, further studies are needed to demonstrate that the “flare-up” effect of deslorelin implant in bitches especially during later prepubertal stages.

Anahtar Kelime: vagina cytology dogs agonists sexual behaviour animal behaviour implantation endocrine system progesterone oestrous cycle oestrus bitches estradiol sodium chloride reproductive behaviour anoestrus blood serum GnRH

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Kaya D, Aslan S, kaya s, KURU M, KACAR C, SOMI SCHAFER S (2013). Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. , 299 - 304.
Chicago	Kaya Duygu,Aslan Selim,kaya semra,KURU Mushap,KACAR Cihan,SOMI SCHAFER Sabine Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. (2013): 299 - 304.
MLA	Kaya Duygu,Aslan Selim,kaya semra,KURU Mushap,KACAR Cihan,SOMI SCHAFER Sabine Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. , 2013, ss.299 - 304.
AMA	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. . 2013; 299 - 304.
Vancouver	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. . 2013; 299 - 304.
IEEE	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S "Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?." , ss.299 - 304, 2013.
ISNAD	Kaya, Duygu vd. "Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?". (2013), 299-304.

APA	Kaya D, Aslan S, kaya s, KURU M, KACAR C, SOMI SCHAFER S (2013). Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 299 - 304.
Chicago	Kaya Duygu,Aslan Selim,kaya semra,KURU Mushap,KACAR Cihan,SOMI SCHAFER Sabine Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.2 (2013): 299 - 304.
MLA	Kaya Duygu,Aslan Selim,kaya semra,KURU Mushap,KACAR Cihan,SOMI SCHAFER Sabine Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.2, 2013, ss.299 - 304.
AMA	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(2): 299 - 304.
Vancouver	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(2): 299 - 304.
IEEE	Kaya D,Aslan S,kaya s,KURU M,KACAR C,SOMI SCHAFER S "Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.299 - 304, 2013.
ISNAD	Kaya, Duygu vd. "Clinical and endocrine short-term effects of GnRH analogue deslorelin in prepubertal bitches: Does a “flare-up” occur?". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/2 (2013), 299-304.