Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey

UNVER, Aysegul; ALTINTAŞ, NAZMİYE; ALTINTAS, NURAY; SAKARYA, ASLAN; ÖZTATLICI, Mustafa

Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey

NURAY ALTINTAŞ, (Celal Bayar Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Manisa, Türkiye)

MUSTAFA ÖZTATLICI, (Celal Bayar Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Manisa, Türkiye)

NAZMİYE ALTINTAŞ, (Ege Üniversitesi, Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Türkiye)

Aysegul UNVER, (Ege Üniversitesi, Tıp Fakültesi, Parazitoloji Anabilim Dalı, İzmir, Türkiye)

Aslan SAKARYA (Celal Bayar Üniversitesi, Tıp Fakültesi, Manisa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

11 1

Yıl: 2013 Cilt: 19 Sayı: 3 Sayfa Aralığı: 455 - 459 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey

Öz:

Echinococcus granulosus insanda ve birçok evcil hayvanda kistik ekinokokkozise (KE) neden olan etkendir ve hala dünyada ve Türkiyede en önemli sağlık problemlerinden biridir. Metasestodlarla infeksiyon şiddetli hastalıklara ve yüksek ekonomik kayıplara neden olur. Bazı Echinococcus suşları, suşların epidemiyolojik ve biyolojik karakteristiklerine dayanarak tanımlanmaktadır. Çalışmamızda sığırlardan elde edilen toplam 18 örnek incelenmiştir. Örnekler 2010-2012 yılları arasında Manisa merkez mezbahasından elde edilmiştir. Total genomik DNA (gDNA) üretici firmanın talimatları doğrultusunda protoskoleks ve kistik germinal membranlardan RTA-DNA İzolasyon Kiti kullanılarak (Gebze/Kocaeli, Türkiye) izole edilmiştir. Bu çalışmanın amacı, Türkiyede Manisa ilindeki sığırlardan elde edilen E. granulosus izolatlarının Polimeraz Zincir Reaksiyonu (PZR) ile moleküler karakterizasyonunun elde edilmesidir. PZRdan sonra, izolatların genetik karakteristiklerini araştırmak için mitokondrial sitokrom c oksidaz alt ünite 1 (CO1) ve nikotinamid adenin dinükleotit dehidrogenaz alt ünite 1 (NAD1) genleri deoksiribonükleik asit dizileme ile ABI Prism Genetik Analizör 3100 cihazıyla çalışıldı. Çalışmamızın sonucu olarak, tüm (18) sığır izolatları E. granulosus sensu stricto (G1-G3 kompleksi) olarak teşhis edildi. Bu çalışma Manisa ilindeki sığırlardan elde edilen Echinococcus izolatlarının ilk moleküler genotiplendirme çalışmasıdır.

Anahtar Kelime: sığır ekinokokozis teknikler DNA Echinococcus granulosus Manisa genom Türkiye NAD parasitoz genotip sığır hastalıkları helmintoz genetik analizler genler

Konular: Veterinerlik

Manisa ilinde Echinococcus granulosus un sığır İzolatlarının moleküler analizi

Öz:

Echinococcus granulosus is the causative agent of cystic echinococcosis (CE) in humans and many domestic animals, and still one of the most important global health problem in the world and in Turkey. Infection with metacestode causes severe illness and high economic losses. Several strains of Echinococcus have been identified based on the epidemiological and biological characteristics of strains. In this study, a total of 18 individual hydatid cyst samples from cattle were examined. They were obtained from central slaughterhouse in the province of Manisa/Turkey between 2010-2012. The total genomic DNA (gDNA) was extracted using RTA-DNA Isolation Kit (Gebze/Kocaeli, Turkey) according to manufacturer instructions from protoscoleces and cystic germinal membranes. The aim of this study was to provide molecular characterization of E. granulosus isolates which were obtained from cattles by using polymerase chain reaction (PCR) in Manisa province of Turkey. After PCR, to investigate the genetic characteristics of isolates, deoxyribonucleic acid sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (NAD1) genes were performed with ABI Prism Genetic Analyzer 3100 instrument. As a result of our study, all (18) cattle isolates were detected as E. granulosus sensu stricto (G1-G3 complex). This is the first molecular study report genotyping of Echinococcus isolates from cattle in Manisa province.

Anahtar Kelime: Manisa genomes Turkey NAD parasitoses genotypes cattle diseases helminthoses genetic analysis genes cattle echinococcosis techniques DNA Echinococcus granulosus

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALTINTAS N, ÖZTATLICI M, ALTINTAŞ N, UNVER A, SAKARYA A (2013). Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. , 455 - 459.
Chicago	ALTINTAS NURAY,ÖZTATLICI Mustafa,ALTINTAŞ NAZMİYE,UNVER Aysegul,SAKARYA ASLAN Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. (2013): 455 - 459.
MLA	ALTINTAS NURAY,ÖZTATLICI Mustafa,ALTINTAŞ NAZMİYE,UNVER Aysegul,SAKARYA ASLAN Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. , 2013, ss.455 - 459.
AMA	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. . 2013; 455 - 459.
Vancouver	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. . 2013; 455 - 459.
IEEE	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A "Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey." , ss.455 - 459, 2013.
ISNAD	ALTINTAS, NURAY vd. "Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey". (2013), 455-459.

APA	ALTINTAS N, ÖZTATLICI M, ALTINTAŞ N, UNVER A, SAKARYA A (2013). Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(3), 455 - 459.
Chicago	ALTINTAS NURAY,ÖZTATLICI Mustafa,ALTINTAŞ NAZMİYE,UNVER Aysegul,SAKARYA ASLAN Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19, no.3 (2013): 455 - 459.
MLA	ALTINTAS NURAY,ÖZTATLICI Mustafa,ALTINTAŞ NAZMİYE,UNVER Aysegul,SAKARYA ASLAN Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.19, no.3, 2013, ss.455 - 459.
AMA	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 455 - 459.
Vancouver	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2013; 19(3): 455 - 459.
IEEE	ALTINTAS N,ÖZTATLICI M,ALTINTAŞ N,UNVER A,SAKARYA A "Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19, ss.455 - 459, 2013.
ISNAD	ALTINTAS, NURAY vd. "Molecular analysis of cattle Isolates of Echinococcus granulosus in Manisa province of Turkey". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 19/3 (2013), 455-459.