Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province

PİŞKİN, Fatma Çiğdem; ÜTÜK, Armağan Erdem; DALKILIÇ, Bestami

Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province

Armağan Erdem ÜTÜK, (Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Parazitoloji Laboratuvarı, Ankara, Türkiye)

Fatma Çiğdem PİŞKİN, (Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Parazitoloji Laboratuvarı, Ankara, Türkiye)

Bestami DALKILIÇ (Gaziantep Üniversitesi, Gaziantep Meslek Yüksek Okulu, Gaziantep, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2012 Cilt: 18 Sayı: (A) Sayfa Aralığı: 35 - 38 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province

Öz:

Kistik ekinokokkozis Türkiye'de ve dünyada en önemli helmintozoonozlardan biridir. Kilis ilindeki 19 koyundan elde edilen 28 Echinococcus granulosus izolatının moleküler karakterizasyonu için mitokondrial 12S rRNA (mt-12S rRNA) ve küçük altünite ribosomal RNA (rrnS) genleri polimeraz zincir reaksiyonu (PZR) ile çoğaltıldı. Ayrıca rastgele seçilen iki koyun izolatının mitokondrial sitokrom c oksidaz altünite 1 (mt-CO1) geni kısmi olarak çoğaltılıp dizi analizi yapıldı. Çalışma sonucunda tüm koyun izolatları E. granulosus sensu stricto (G1-G3 kompleks) olarak belirlendi

Anahtar Kelime:

Konular: Veterinerlik

Kilis İlinde Echinococcus granulosus'un Koyun İzolatlarının Moleküler Karakterizasyonu

Öz:

Cystic echinococcosis is one of the most important helminthozoonosis both in Turkey and all around world. Polymerase Chain Reaction (PCR) amplification of mitochondrial 12S rRNA (mt-12S rRNA) and small subunit ribosomal RNA (rrnS) genes were used for molecular characterization of 28 isolates of Echinococcus granulosus obtained from 19 sheep in Kilis province. And also partial mitochondrial cytochrome c oxidase subunit I (mt-CO1) genes of randomly selected two sheep isolates were amplified and sequenced. At the end of the study, all sheep isolates were detected as E. granulosus sensu stricto (G1-G3 complex)

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÜTÜK A, PİŞKİN F, DALKILIÇ B (2012). Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. , 35 - 38.
Chicago	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,DALKILIÇ Bestami Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. (2012): 35 - 38.
MLA	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,DALKILIÇ Bestami Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. , 2012, ss.35 - 38.
AMA	ÜTÜK A,PİŞKİN F,DALKILIÇ B Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. . 2012; 35 - 38.
Vancouver	ÜTÜK A,PİŞKİN F,DALKILIÇ B Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. . 2012; 35 - 38.
IEEE	ÜTÜK A,PİŞKİN F,DALKILIÇ B "Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province." , ss.35 - 38, 2012.
ISNAD	ÜTÜK, Armağan Erdem vd. "Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province". (2012), 35-38.

APA	ÜTÜK A, PİŞKİN F, DALKILIÇ B (2012). Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18((A)), 35 - 38.
Chicago	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,DALKILIÇ Bestami Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18, no.(A) (2012): 35 - 38.
MLA	ÜTÜK Armağan Erdem,PİŞKİN Fatma Çiğdem,DALKILIÇ Bestami Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.18, no.(A), 2012, ss.35 - 38.
AMA	ÜTÜK A,PİŞKİN F,DALKILIÇ B Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2012; 18((A)): 35 - 38.
Vancouver	ÜTÜK A,PİŞKİN F,DALKILIÇ B Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2012; 18((A)): 35 - 38.
IEEE	ÜTÜK A,PİŞKİN F,DALKILIÇ B "Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 18, ss.35 - 38, 2012.
ISNAD	ÜTÜK, Armağan Erdem vd. "Molecular Characterization of Sheep Isolates of Echinococcus granulosus in Kilis Province". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 18/(A) (2012), 35-38.