Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)

Sevinç BAŞAY, (Uludağ Üniversitesi, Karacabey Meslek Yüksekokulu, Bursa, Türkiye)
Şeküre Şebnem ELLİALTIOĞLU (Ankara Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Ankara, Türkiye)
Turkish Journal of Biology
13 0
Yıl: 2013 Cilt: 37 Sayı: 4 Sayfa Aralığı: 499 - 505 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)

Öz:
The aim of this study was to determine the androgenic capacity of some eggplant (Solanum melongena L.) varieties and breeding lines, and to establish the effectiveness of androgenesis induction in the less responsive genotypes by crossing with more responsive genotypes that have a high ability for haploid embryo formation. Flower buds containing greenish-yellow anthers were collected when the microspores were at the late uninucleate stage, which is equivalent to a bud size where petals are not visible. The anthers were kept in the dark at 35 °C for 8 days, then incubated with a 12 h light period at 25 °C for 4 days on C medium, and then transferred to solid R medium, supplemented with 30 g/L sucrose and 0.1 mg/L kinetin. Topan, Halep Karası, and Teorem $F_{1}$ varieties and 2 breeding lines [Vd-1 and Vd-2 (LS 2346)] that have a tolerance to Verticillium dahliae Kleb. were used as the plant materials during the first part of the study. Haploid embryos were obtained from Topan and Halep Karası at the rate of 4.16% and 2.63%, respectively. The responses of Topan and Halep Karası eggplant varieties to the anther culture were better than those of the Teorem $F_{1}$ cultivar and the Vd-1 and Vd-2 lines. Because of their responses to anther culture, the Topan and Halep Karası varieties were used as donor parents, and they were crossed with the other 3 genotypes (Teorem $F_{1}$, Vd-1, and Vd-2) reciprocally. Gametic embryogenesis among hybrids was only obtained from the Topan × Teorem $F_{1}$ and Teorem $F_{1}$ × Topan combinations (0.87% and 2.57%, respectively). Development of the haploid embryos and plant formation occurred at the rates of 0.69% and 2.57%, respectively. This study revealed that the effectiveness of androgenesis in eggplant strongly depended on genotypes. This crossing technique could be useful for increasing the opportunity for haploid embryogenesis from genotypes that are unlikely to form haploid embryos, and crossing between the fair-responsive and poor/ nonresponsive genotypes could be a good system for qualifying androgenic response in eggplant.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Başay S, ELLİALTIOĞLU Ş (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). , 499 - 505.
Chicago Başay Sevinç,ELLİALTIOĞLU Şeküre Şebnem Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). (2013): 499 - 505.
MLA Başay Sevinç,ELLİALTIOĞLU Şeküre Şebnem Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). , 2013, ss.499 - 505.
AMA Başay S,ELLİALTIOĞLU Ş Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). . 2013; 499 - 505.
Vancouver Başay S,ELLİALTIOĞLU Ş Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). . 2013; 499 - 505.
IEEE Başay S,ELLİALTIOĞLU Ş "Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)." , ss.499 - 505, 2013.
ISNAD Başay, Sevinç - ELLİALTIOĞLU, Şeküre Şebnem. "Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)". (2013), 499-505.
APA Başay S, ELLİALTIOĞLU Ş (2013). Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology, 37(4), 499 - 505.
Chicago Başay Sevinç,ELLİALTIOĞLU Şeküre Şebnem Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology 37, no.4 (2013): 499 - 505.
MLA Başay Sevinç,ELLİALTIOĞLU Şeküre Şebnem Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology, vol.37, no.4, 2013, ss.499 - 505.
AMA Başay S,ELLİALTIOĞLU Ş Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology. 2013; 37(4): 499 - 505.
Vancouver Başay S,ELLİALTIOĞLU Ş Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.). Turkish Journal of Biology. 2013; 37(4): 499 - 505.
IEEE Başay S,ELLİALTIOĞLU Ş "Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)." Turkish Journal of Biology, 37, ss.499 - 505, 2013.
ISNAD Başay, Sevinç - ELLİALTIOĞLU, Şeküre Şebnem. "Effect of genotypical factors on the effectiveness of anther culture in eggplant (Solanum melongena L.)". Turkish Journal of Biology 37/4 (2013), 499-505.
Sayfa Oluşturulma Tarihi
22-04-2024 19:3

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