Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)

Doğan, Dilek; COLAKOGLU, Feyza; SİVRİ, Nüket; SANDALLI, Cemal; ÖZGÜMÜŞ, OSMAN BİROL

Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)

Nüket SİVRİ, (İstanbul Üniversitesi, Mühendislik Fakültesi, Çevre Mühendisliği Bölümü, İstanbul, Türkiye)

Cemal SANDALLI, (Recep Tayyip Erdoğan Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

OSMAN BİROL ÖZGÜMÜŞ, (Recep Tayyip Erdoğan Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Rize, Türkiye)

Feyza COLAKOGLU, (Recep Tayyip Erdoğan Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Dilek DOĞAN (Akal Makine ve Kimya Endüstrisi, Barbaros Bulvarı, Beşiktaş, 34349, İstanbul, Türkiye)

Turkish Journal of Fisheries and Aquatic Sciences

3 0

Yıl: 2012 Cilt: 12 Sayı: 3 Sayfa Aralığı: 699 - 707 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)

Öz:

Bu çalışmanın amacı, Küçükçekmece Lagünündeki (İstanbul) fekal bakteri yoğunluğunu bulmak ve izole edilen Gram negatif basillerin antibiyotiklere direncini analiz etmektir. Örnekler, Haziran 2006'dan Haziran 2008 tarihleri arasında aylık olarak alınmış ve toplam 232 Gram negatif basil izole edilmiştir. Antimikrobial hassasiyet testleri için kloramfenikol, tetrasiklin, nalidiksik asit, ampisilin, imipenem, seftazidim, amikasin, streptomisin ve amoksisilin+klavulanik asit kullanılmıştır. Trimetoprim+sulfametoksazola yatkınlık da sadece integron taşıyan organizmalar için incelenmiştir. Antibiyotik direnç sonuçlarına göre bakterilerin en dirençli olduğu antibiyotik ampisilin (%76,29) ve en hassas olduğu antibiyotik ise amikasin (%6,44) olarak bulunmuştur. 232 izolatın 20 si (%8,6) sınıf 1 ve / veya sınıf 2 integron barındırmıştır. DNA sekanslama sonucunda, integronlara ait değişken bölgelerin barındırdığı gen kasetlerinin, DfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, blaOXA-30 ve sat2 olduğu tespit edilmiştir. Bakterilere ait bu integronlar 12 ve D3 nolu istasyonlar dışındaki tüm örnekleme bölgelerinde bulunmuştur. Bu çalışmada, Küçükçekmece Lagününde Gram negatif bakteri türlerinin identifikasyonu ve antibiyotik direnç profili ile ilgili çalışma ilk kez yapılmıştır. Muhtemelen yoğun antropojenik girdilerin sonucu olarak Lagünde ağır bir fekal kirlilik olduğu görülmüştür. Atık su arıtma tesisleri gibi etkili önlemler alınmazsa, insanların normal mikrobiotasında yer alan bakteri izolatlarına, antibiyotik direnç genlerinin aktarımı ile halk sağlığı riski oluşması olasıdır.

Anahtar Kelime:

Konular: Balıkçılık Zooloji

Küçükçekmece Lagünü nden (İstanbul- Türkiye) izole edilen enterik bakterilerin antibiyotik direnç profilleri

Öz:

The aim of this study is to find out the density of the fecal bacteria and to analyze resistance to antimicrobials of Gram negative bacilli isolated from the Kucukcekmece Lagoon, Istanbul. Samples were taken monthly from June 2006 to June 2008 and a total of 232 Gram negative bacilli were isolated. Chloramphenicol, tetracycline, nalidixic acid, ampicillin, imipenem, ceftazidime, amikacin, streptomycin and amoxicillin + clavulanic acid were used in antimicrobial susceptibility tests. Susceptibility to trimethoprim + sulfamethoxazole was also examined in only integron-bearing organisms. The antibiotic resistance tests resulted in bacteria being the most resistant against ampicillin (76.29%) and the most sensitive against amikacin (93.56%). Of 232 isolates, 20 (8.6%) coliforms harbored class 1 and/or class 2 integrons. DNA sequencing showed that variable regions of the integrons harbored various gene cassettes; dfrA12, dfrA15, dfrA17, aadA1, aadA2, aadA5, blaOXA-30 and sat2. Integrons were found in bacteria from all sampling areas except 12 and D3. In this study, the determination of bacterial identification of the species of Gram negative bacilli and their Antibiotic Resistance Profiles in the Kucukcekmece Lagoon for the first time was investigated. A finding indicates that there is a heavy fecal pollution in this lagoon environment, which might probably be resulted from the intensive anthropogenic facilities. The risk to public health could be the transfer of antibiotic resistance determinants from the bacterial isolates to normal microbiota bacteria of humans unless the effective precautions such as water treatment plants are taken.

Anahtar Kelime:

Konular: Balıkçılık Zooloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SİVRİ N, SANDALLI C, ÖZGÜMÜŞ O, COLAKOGLU F, Doğan D (2012). Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). , 699 - 707.
Chicago	SİVRİ Nüket,SANDALLI Cemal,ÖZGÜMÜŞ OSMAN BİROL,COLAKOGLU Feyza,Doğan Dilek Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). (2012): 699 - 707.
MLA	SİVRİ Nüket,SANDALLI Cemal,ÖZGÜMÜŞ OSMAN BİROL,COLAKOGLU Feyza,Doğan Dilek Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). , 2012, ss.699 - 707.
AMA	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). . 2012; 699 - 707.
Vancouver	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). . 2012; 699 - 707.
IEEE	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D "Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)." , ss.699 - 707, 2012.
ISNAD	SİVRİ, Nüket vd. "Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)". (2012), 699-707.

APA	SİVRİ N, SANDALLI C, ÖZGÜMÜŞ O, COLAKOGLU F, Doğan D (2012). Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). Turkish Journal of Fisheries and Aquatic Sciences, 12(3), 699 - 707.
Chicago	SİVRİ Nüket,SANDALLI Cemal,ÖZGÜMÜŞ OSMAN BİROL,COLAKOGLU Feyza,Doğan Dilek Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). Turkish Journal of Fisheries and Aquatic Sciences 12, no.3 (2012): 699 - 707.
MLA	SİVRİ Nüket,SANDALLI Cemal,ÖZGÜMÜŞ OSMAN BİROL,COLAKOGLU Feyza,Doğan Dilek Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). Turkish Journal of Fisheries and Aquatic Sciences, vol.12, no.3, 2012, ss.699 - 707.
AMA	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). Turkish Journal of Fisheries and Aquatic Sciences. 2012; 12(3): 699 - 707.
Vancouver	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey). Turkish Journal of Fisheries and Aquatic Sciences. 2012; 12(3): 699 - 707.
IEEE	SİVRİ N,SANDALLI C,ÖZGÜMÜŞ O,COLAKOGLU F,Doğan D "Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)." Turkish Journal of Fisheries and Aquatic Sciences, 12, ss.699 - 707, 2012.
ISNAD	SİVRİ, Nüket vd. "Antibiotic resistance profiles of enteric bacteria Isolated from Kucukcekmece Lagoon (IstanbulTurkey)". Turkish Journal of Fisheries and Aquatic Sciences 12/3 (2012), 699-707.