It is important to determine the perceived burden of care and the difficulties facing caregivers of the children with chronic disease because of the increase in its incidence in childhood. This descriptive study was conducted to determine the burden of care experienced by the mothers of children with chronic disease. The study sample consisted of the mothers (n=73) of 7-18 years old children with epilepsy, diabetes mellitus (DM) and chronic renal failure (CRF) (epilepsy=26, DM=26, CRF=21) followed up in Pediatric Neurology, Endocrinology and Nephrology clinics of a university hospital. The data was collected with a questionnaire form and Caregiver Burden Inventory. For the analysis of data; Chi-square, Student-t, Kruskal-Wallis and Mann-Witney U tests were used. It was determined that the mean age of the mothers was 38.16±6.13 years (24-56), and the duration of the child's disease was 4.98±3.40 years (1-14). The mean scores of burden of time-dependency, developmental, physical, social and emotional subscales of burden inventory and total scale of the mothers of children with DM were high (p<0.05). It was detected that perceived burden of care was affected by the presence of other health problems and the age of the child with chronic disease, their health problems and the age of the mothers, economic difficulties and giving care to another person except the child with chronic disease (p<0.05). The perceived burden of care experienced by the mothers of children with chronic disease was high. In conclusion, it is recommended that the support should be given to the mothers in the treatment and care of their children.