Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

JALALPOUR, Zohreh; SHARIFI TEHRANI, Majid; SHABANI, Leila; amini, sayed asadollah; AFGHANI, Ladan

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Zohreh JALALPOUR, (Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran)

Leila SHABANI, (Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran)

Ladan AFGHANI, (Department of Biology, Faculty of Sciences, Shahrekord University, Shahrekord, Iran)

Majid SHARIFI TEHRANI, (Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran)

Sayed Asadollah AMINI (Department of Biochemistry, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran)

Turkish Journal of Biology

3 0

Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 76 - 82 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Öz:

Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonia- lyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA (100 μM), S (0.1 μM), and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	JALALPOUR Z, SHABANI L, AFGHANI L, SHARIFI TEHRANI M, amini s (2014). Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. , 76 - 82.
Chicago	JALALPOUR Zohreh,SHABANI Leila,AFGHANI Ladan,SHARIFI TEHRANI Majid,amini sayed asadollah Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. (2014): 76 - 82.
MLA	JALALPOUR Zohreh,SHABANI Leila,AFGHANI Ladan,SHARIFI TEHRANI Majid,amini sayed asadollah Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. , 2014, ss.76 - 82.
AMA	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. . 2014; 76 - 82.
Vancouver	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. . 2014; 76 - 82.
IEEE	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s "Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew." , ss.76 - 82, 2014.
ISNAD	JALALPOUR, Zohreh vd. "Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew". (2014), 76-82.

APA	JALALPOUR Z, SHABANI L, AFGHANI L, SHARIFI TEHRANI M, amini s (2014). Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turkish Journal of Biology, 38(1), 76 - 82.
Chicago	JALALPOUR Zohreh,SHABANI Leila,AFGHANI Ladan,SHARIFI TEHRANI Majid,amini sayed asadollah Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turkish Journal of Biology 38, no.1 (2014): 76 - 82.
MLA	JALALPOUR Zohreh,SHABANI Leila,AFGHANI Ladan,SHARIFI TEHRANI Majid,amini sayed asadollah Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turkish Journal of Biology, vol.38, no.1, 2014, ss.76 - 82.
AMA	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turkish Journal of Biology. 2014; 38(1): 76 - 82.
Vancouver	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew. Turkish Journal of Biology. 2014; 38(1): 76 - 82.
IEEE	JALALPOUR Z,SHABANI L,AFGHANI L,SHARIFI TEHRANI M,amini s "Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew." Turkish Journal of Biology, 38, ss.76 - 82, 2014.
ISNAD	JALALPOUR, Zohreh vd. "Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew". Turkish Journal of Biology 38/1 (2014), 76-82.