Study of in vitro anther culture in selected genotypes of genus Capsicum

Dorota OLSZEWSKA, (Department of Genetics, Physiology, and Plant Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland)
Anna KISIALA, (Department of Genetics, Physiology, and Plant Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland)
Aleksandra NIKLAS NOWAK, (Department of Genetics, Physiology, and Plant Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland)
Pawel NOWACZYK (Department of Genetics, Physiology, and Plant Biotechnology, Faculty of Agriculture and Biotechnology, University of Technology and Life Sciences, Bydgoszcz, Poland)
Turkish Journal of Biology
3 0
Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 118 - 124 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Study of in vitro anther culture in selected genotypes of genus Capsicum

Öz:
The combined effect of anther incubation time on CP induction medium (12, 14, and 16 days) and 2 concentrations of kinetin in R1 regeneration medium (0.1 and 0.3 mg/L) on the effectiveness of androgenesis was investigated in 17 genotypes of Capsicum grown in Poland. Plant material consisted of breeding lines and intraspecific hybrids of C. annuum; the species of C. frutescens, C. chinense, and C. baccatum var. pendulum; interspecific hybrids F1 (C. frutescens × C. chinense) and F1 (C. frutescens × C. baccatum); and doubled haploid lines derived from the hybrids. The results of the tested variants of the experiment were compared with the androgenic response of the control anthers cultured according to the standard protocol previously developed for pepper (12 days incubation on CP, 0.1 mg/L kinetin in R1). Under control conditions, androgenic embryos regenerated only from anthers of 3 of the tested genotypes, whereas application of the selected modifications promoted embryo development in an additional 12 genotypes. The highest effectiveness of androgenesis was observed after 16 days of anther incubation on CP medium combined with 0.1 mg/L kinetin in R1 medium. Twelve- and 14-day-long anther incubation was more effective when followed by transferring anthers onto R1 medium supplemented with 0.3 mg/L kinetin.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA OLSZEWSKA D, KISIALA A, NIKLAS NOWAK A, NOWACZYK P (2014). Study of in vitro anther culture in selected genotypes of genus Capsicum. , 118 - 124.
Chicago OLSZEWSKA Dorota,KISIALA Anna,NIKLAS NOWAK Aleksandra,NOWACZYK Pawel Study of in vitro anther culture in selected genotypes of genus Capsicum. (2014): 118 - 124.
MLA OLSZEWSKA Dorota,KISIALA Anna,NIKLAS NOWAK Aleksandra,NOWACZYK Pawel Study of in vitro anther culture in selected genotypes of genus Capsicum. , 2014, ss.118 - 124.
AMA OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P Study of in vitro anther culture in selected genotypes of genus Capsicum. . 2014; 118 - 124.
Vancouver OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P Study of in vitro anther culture in selected genotypes of genus Capsicum. . 2014; 118 - 124.
IEEE OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P "Study of in vitro anther culture in selected genotypes of genus Capsicum." , ss.118 - 124, 2014.
ISNAD OLSZEWSKA, Dorota vd. "Study of in vitro anther culture in selected genotypes of genus Capsicum". (2014), 118-124.
APA OLSZEWSKA D, KISIALA A, NIKLAS NOWAK A, NOWACZYK P (2014). Study of in vitro anther culture in selected genotypes of genus Capsicum. Turkish Journal of Biology, 38(1), 118 - 124.
Chicago OLSZEWSKA Dorota,KISIALA Anna,NIKLAS NOWAK Aleksandra,NOWACZYK Pawel Study of in vitro anther culture in selected genotypes of genus Capsicum. Turkish Journal of Biology 38, no.1 (2014): 118 - 124.
MLA OLSZEWSKA Dorota,KISIALA Anna,NIKLAS NOWAK Aleksandra,NOWACZYK Pawel Study of in vitro anther culture in selected genotypes of genus Capsicum. Turkish Journal of Biology, vol.38, no.1, 2014, ss.118 - 124.
AMA OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P Study of in vitro anther culture in selected genotypes of genus Capsicum. Turkish Journal of Biology. 2014; 38(1): 118 - 124.
Vancouver OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P Study of in vitro anther culture in selected genotypes of genus Capsicum. Turkish Journal of Biology. 2014; 38(1): 118 - 124.
IEEE OLSZEWSKA D,KISIALA A,NIKLAS NOWAK A,NOWACZYK P "Study of in vitro anther culture in selected genotypes of genus Capsicum." Turkish Journal of Biology, 38, ss.118 - 124, 2014.
ISNAD OLSZEWSKA, Dorota vd. "Study of in vitro anther culture in selected genotypes of genus Capsicum". Turkish Journal of Biology 38/1 (2014), 118-124.
Sayfa Oluşturulma Tarihi
22-04-2024 19:40

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