Investigation of relationships between DNA integrity and fresh semen parameters in rams

Soylu, Mustafa Kemal; TOKER, Berk; NUR, ZEKARIYA; ALÇAY, SELİM; SAĞIRKAYA, Hakan; ÜSTÜNER, BURCU

Investigation of relationships between DNA integrity and fresh semen parameters in rams

Selim ALÇAY, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Berk TOKER, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Burcu ÜSTÜNER, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Zekariya NUR, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Hakan SAĞIRKAYA, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Mustafa Kemal SOYLU (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

11 0

Yıl: 2014 Cilt: 20 Sayı: 5 Sayfa Aralığı: 793 - 798 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation of relationships between DNA integrity and fresh semen parameters in rams

Öz:

Bu çalışmanın amacı, Kıvırcık ve İvesi ırkı koçlardan alınan spermaların, sezon içerisinde rutin sperma analizleri ve TUNEL ile yapılansperm DNA bütünlüğü arasındaki korelasyonu değerlendirmektir. Sperma elektro-ejakülasyon yöntemiyle 5 kez ve birer gün aralıklarlaalındı. Bu amaçla; 6 baş Kıvırcık ve 4 baş İvesi ırkı koçtan alınan 50 ejakülat değerlendirildi. Kıvırcık ve İvesi ırkı koçların ortalama spermahacmi, konsantrasyonu, mass aktivitesi, motilitesi, akrozomal bozukluğu, HOST ve DNA fragmantasyonu yüzdeleri sırasıyla 1.3 ml, 1.9x109, 3.3 (0-5 skala), %72.6, %13.2, %73.6, %16.2 ve 1.0 ml, 1.9 x109, 3.3 (0-5 skala), %72.5, %11.1, %77.9 ve %15.9 olarak bulundu. SpermDNA fragmantasyonu; sperma hacmi (r = -0.329, P<0.05), sperma konsantrasyonu (r = -0.188, P>0.05), mass aktivite (r = -0.349, P<0.05),motilite (r = -0.448, P<0.01), akrozomal bozukluk (r = -0.103, P>0.05) ve HOS testi (r= -0.513, P<0.01) ile negatif korelasyon gösterdi. Buçalışma, değerlendirilen çoğu parametrenin DNA fragmantasyonu ile bağlantılı olduğunu açıkça gösterdi. Dolayısıyla, DNA yapısınınincelenmesi her bir koç ejakülatının fertilitesinin doğru olarak tespit edilmesi konusunda faydalı olabilir.

Anahtar Kelime:

Konular: Veterinerlik

Koçlarda taze sperma parametreleri ve DNA bütünlüğü arasındaki ilişkilerin araştırılması

Öz:

The aim of the present study was to evaluate the correlation between the routine semen analyses and sperm DNA integrity, as assesedby TUNEL in fresh semen of rams in Kivircik and Awassi breeds in breeding season. Semen was collected by electro-ejaculation five timesevery other day. For that purpose, 50 fresh ejaculates from 6 Kivircik and 4 Awassi rams were evaluated. The mean percentages of semenvolume, concentration, mass activity, motility, acrosomal defects, HOST and DNA fragmentation of Kivircik and Awassi rams were 1.3 ml,1.9 x109, 3.3 (0-5 scale), 72.6%, 13.2%, 73.6%, 16.2% and 1.0 ml, 1.9 x109, 3.3 (0-5 scale), 72.5%, 11.1%, 77.9%, 15.9%; respectively (P>0.05).Sperm DNA fragmentation was correlated adversely with semen volume (r = -0.329, P<0.05), concentration (r = -0.188, P>0.05), massactivity (r = -0.349, P<0.05), motility (r = -0.448, P<0.01), acrosomal defects (r = -0.103, P>0.05) and HOS test (r = -0.513, P<0.01). This studyprovided clear evidence that most of the parameters evaluated herein related to DNA fragmentation at one point or the other. Hence,DNA structure evaluation of sperm might be a useful tool for accurate prediction of the male fertility in individual rams.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALÇAY S, TOKER B, ÜSTÜNER B, NUR Z, SAĞIRKAYA H, Soylu M (2014). Investigation of relationships between DNA integrity and fresh semen parameters in rams. , 793 - 798.
Chicago	ALÇAY SELİM,TOKER Berk,ÜSTÜNER BURCU,NUR ZEKARIYA,SAĞIRKAYA Hakan,Soylu Mustafa Kemal Investigation of relationships between DNA integrity and fresh semen parameters in rams. (2014): 793 - 798.
MLA	ALÇAY SELİM,TOKER Berk,ÜSTÜNER BURCU,NUR ZEKARIYA,SAĞIRKAYA Hakan,Soylu Mustafa Kemal Investigation of relationships between DNA integrity and fresh semen parameters in rams. , 2014, ss.793 - 798.
AMA	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M Investigation of relationships between DNA integrity and fresh semen parameters in rams. . 2014; 793 - 798.
Vancouver	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M Investigation of relationships between DNA integrity and fresh semen parameters in rams. . 2014; 793 - 798.
IEEE	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M "Investigation of relationships between DNA integrity and fresh semen parameters in rams." , ss.793 - 798, 2014.
ISNAD	ALÇAY, SELİM vd. "Investigation of relationships between DNA integrity and fresh semen parameters in rams". (2014), 793-798.

APA	ALÇAY S, TOKER B, ÜSTÜNER B, NUR Z, SAĞIRKAYA H, Soylu M (2014). Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(5), 793 - 798.
Chicago	ALÇAY SELİM,TOKER Berk,ÜSTÜNER BURCU,NUR ZEKARIYA,SAĞIRKAYA Hakan,Soylu Mustafa Kemal Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20, no.5 (2014): 793 - 798.
MLA	ALÇAY SELİM,TOKER Berk,ÜSTÜNER BURCU,NUR ZEKARIYA,SAĞIRKAYA Hakan,Soylu Mustafa Kemal Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.20, no.5, 2014, ss.793 - 798.
AMA	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(5): 793 - 798.
Vancouver	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(5): 793 - 798.
IEEE	ALÇAY S,TOKER B,ÜSTÜNER B,NUR Z,SAĞIRKAYA H,Soylu M "Investigation of relationships between DNA integrity and fresh semen parameters in rams." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20, ss.793 - 798, 2014.
ISNAD	ALÇAY, SELİM vd. "Investigation of relationships between DNA integrity and fresh semen parameters in rams". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20/5 (2014), 793-798.