Screening the antifungal activity of soilborne actinomycetes

KIVANÇ, Merih; DEMİREL, RASİME; TAY, Turgay; YILMAZ SARIÖZLÜ, NALAN

Screening the antifungal activity of soilborne actinomycetes

Rasime DEMİREL, (Anadolu Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Nalan YILMAZ SARIÖZLÜ, (Anadolu Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Turgay TAY, (Anadolu Üniversitesi, Fen Fakültesi, Kimya Bölümü, Eskişehir, Türkiye)

Merih KIVANÇ (Anadolu Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Biological Diversity and Conservation

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Yıl: 2016 Cilt: 9 Sayı: 2 Sayfa Aralığı: 207 - 213 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Screening the antifungal activity of soilborne actinomycetes

Öz:

Türkiye'nin batısından toplanan 35 toprak örneğinden izole edilen toplam 288 aktinomiset suşu Aspergillus niger (ATCC-1094), A. parasiticus (NRRL-465), Fusarium moniliforme (NRRL 1866), F. solani (NRRL-13414), Penicillium chrysogenum (NRRL 807), A. fumigatus, Alternaria citri, Al. tenuissima, Athelia rolfsii, F. acuminatum, F. aveneceum, F. clamydosporium, F. culmarum, F. equiseti, F. oxysporum, F. semitectum, F. subglutanas, Fusarium sp., Gaeumannomyces graminis, Mycosphaerella rabiei, P. citrinum ve P. piceum gibi hasat öncesi ve sonrası fungal patojenlere karşı test edilmiştir. Birinci taramada, test edilen suşların % 30,55'i fungal patojenlere karşı inhibisyon potansiyeli göstermiştir. Etkin izolatlardan 7 suş birinci taramada yüksek inhibisyon potansiyellerine dayalı olarak dört farklı fermantasyon ortamı ile ikinci tarama için seçilmiştir. Fermantasyon B ortamının hücresiz süpernatantı test funguslarına karşı geniş spectrum ve yüksek inhibisyon potansiyeli göstermiştir. Etil asetat ile ektraksiyondan sonra, seçilen yedi suştan XF, BF, AM3.1, 9M ve 14M özellikle bazı Aspergillus ve Fusarium üyelerine karşı antifungal aktivite göstermişlerdir. Bu sonuçlar; test edilen beş aktinomiset suşunun hasat öncesi ve sonrası fungal patojenlere karşı potansiyel aktiviteye sahip olduğunu, zirai ürünlerin korunması için kullanışlı olabilecek antagonistik bileşiklerinin üretilebilir ve antifungal aktivitelerini kaybetmeden elde edilebilir olduğunu işaret etmektedir.

Anahtar Kelime:

Konular: Biyoloji

Toprak kaynaklı aktinomisetlerin antifungal aktivitesinin taranması

Öz:

A total of 288 actinomycete strains, isolated from 35 soil samples collected from western Turkey, were tested against pre- and post-harvest fungal pathogens such as Aspergillus niger (ATCC-1094), A. parasiticus (NRRL-465), Fusarium moniliforme (NRRL 1866), F. solani (NRRL-13414), Penicillium chrysogenum (NRRL 807), A. fumigatus, Alternaria citri, Al. tenuissima, Athelia rolfsii, F. acuminatum, F. aveneceum, F. clamydosporium, F. culmarum, F. equiseti, F. oxysporum, F. semitectum, F. subglutanas, Fusarium sp., Gaeumannomyces graminis, Mycosphaerella rabiei, P. citrinum and P. piceum. During primary screening, 30.55% of the strains showed inhibition potential against the test pathogen fungi. Of the effective isolates, seven strains were selected for secondary screening with four different fermentation media based on their high inhibition potential in primary screening. The cell-free supernatant of fermentation B medium showed broad-spectrum and high inhibition potential against the test fungi. After extraction with ethyl acetate, XF, BF, AM3.1, 9M and 14M from the selected seven strains exhibited antifungal activity against particularly some members of Fusarium and Aspergillus genera. These results indicate that the tested five actinomycete strains have potential activity against pre- and post-harvest pathogens, and their antagonist compounds can be produced and obtained without the loss of antifungal effects, which could be useful for the protection of agricultural products.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DEMİREL R, YILMAZ SARIÖZLÜ N, TAY T, KIVANÇ M (2016). Screening the antifungal activity of soilborne actinomycetes. , 207 - 213.
Chicago	DEMİREL RASİME,YILMAZ SARIÖZLÜ NALAN,TAY Turgay,KIVANÇ Merih Screening the antifungal activity of soilborne actinomycetes. (2016): 207 - 213.
MLA	DEMİREL RASİME,YILMAZ SARIÖZLÜ NALAN,TAY Turgay,KIVANÇ Merih Screening the antifungal activity of soilborne actinomycetes. , 2016, ss.207 - 213.
AMA	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M Screening the antifungal activity of soilborne actinomycetes. . 2016; 207 - 213.
Vancouver	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M Screening the antifungal activity of soilborne actinomycetes. . 2016; 207 - 213.
IEEE	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M "Screening the antifungal activity of soilborne actinomycetes." , ss.207 - 213, 2016.
ISNAD	DEMİREL, RASİME vd. "Screening the antifungal activity of soilborne actinomycetes". (2016), 207-213.

APA	DEMİREL R, YILMAZ SARIÖZLÜ N, TAY T, KIVANÇ M (2016). Screening the antifungal activity of soilborne actinomycetes. Biological Diversity and Conservation, 9(2), 207 - 213.
Chicago	DEMİREL RASİME,YILMAZ SARIÖZLÜ NALAN,TAY Turgay,KIVANÇ Merih Screening the antifungal activity of soilborne actinomycetes. Biological Diversity and Conservation 9, no.2 (2016): 207 - 213.
MLA	DEMİREL RASİME,YILMAZ SARIÖZLÜ NALAN,TAY Turgay,KIVANÇ Merih Screening the antifungal activity of soilborne actinomycetes. Biological Diversity and Conservation, vol.9, no.2, 2016, ss.207 - 213.
AMA	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M Screening the antifungal activity of soilborne actinomycetes. Biological Diversity and Conservation. 2016; 9(2): 207 - 213.
Vancouver	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M Screening the antifungal activity of soilborne actinomycetes. Biological Diversity and Conservation. 2016; 9(2): 207 - 213.
IEEE	DEMİREL R,YILMAZ SARIÖZLÜ N,TAY T,KIVANÇ M "Screening the antifungal activity of soilborne actinomycetes." Biological Diversity and Conservation, 9, ss.207 - 213, 2016.
ISNAD	DEMİREL, RASİME vd. "Screening the antifungal activity of soilborne actinomycetes". Biological Diversity and Conservation 9/2 (2016), 207-213.