Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Tugba YİLMAZ OZDEN, (İstanbul Üniversitesi, Eczacılık Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)
Ayşe CAN, (İstanbul Üniversitesi, Eczacılık Fakültesi, Biyokimya Bölümü, İstanbul, Türkiye)
Serap SANCAR BAŞ, (İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Türkiye)
Zeliha KARA PALA, (İstanbul Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye)
Alper OKYAR, (İstanbul Üniversitesi, Eczacılık Fakültesi, Farmakoloji Anabilim Dalı, İstanbul, Türkiye)
Şehnaz BOLKENT (İstanbul Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, İstanbul, Türkiye)
Türk Biyokimya Dergisi
4 0
Yıl: 2015 Cilt: 40 Sayı: 2 Sayfa Aralığı: 125 - 131 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats

Öz:
Amaç: Amaranthus lividus, Türkiye'nin Batı Karadeniz Bölgesinde sebze olarak yaygın şekilde tüketilen bir bitkidir. Bu çalışmada A. lividus'un karbon tetraklorür (CCl4) ile sıçanlarda oluşturulan oksidatif stres ve akut karaciğer hasarı üzerine koruyucu ve antioksidan etkilerinin ölçülmesini amaçladık.Metod: Erkek albino Wistar cinsi sıçanlar 7 gruba ayrıldı: Normal kontrol, A. lividus kontrol, silimarin kontrol, CCl4, A. lividus (250 mg/kg)+CCl4, A. lividus (500 mg/kg)+CCl4, silimarin+CCl4. Sıçanlara CCl4 (1.5 mL/kg, 1:1 zeytin yağı içinde, i.p.) verilmeden önce 9 gün boyunca günde 1 kere ağızdan A. lividus (250 ve 500 mg/kg) veya silimarin (25 mg/kg)'in ön uygulaması yapıldı.Bulgular: Sıçanlara A. lividus ile ön uygulama yapılması, serumdaki alanin aminotrasferaz, aspartat aminotransferaz, bilirubin ve karaciğerdeki lipit peroksidasyonu ve miyeloperoksidaz düzeylerinde CCl4'ün neden olduğu artışı anlamlı şekilde önledi. İlave olarak A. lividus ile ön uygulama yapılması, karaciğerdeki katalaz, glutatyon-S-transferaz, glutatyon peroksidaz, glutatyon redüktaz ve süperoksit dismutaz gibi antioksidan enzim aktivitelerinde ve glutatyon düzeyinde CCl4'ün neden olduğu azalışı anlamlı şekilde önledi. A. lividus ve standart ilaç olarak kullanılan silimarinin karaciğer üzerinde benzer koruyucu etkiler gösterdiği gözlenmiştir. Ayrıca karaciğerin histopatolojik olarak değerlendirilmesi, 250 mg/kg dozda A. lividus'un CCl4nedeni ile sıçanların karaciğerinde oluşan hasarı kısmen baskıladığını ortaya koymuştur. Sonuç: Bulgularımız, CCl4 ile sıçanlarda oluşturulan akut karaciğer toksisitesine karşı A. lividus'un koruyucu etkiye sahip olduğunu ve bu etkinin antioksidan aktivitesi ile bağlantılı olabileceğini göstermektedir.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

[Karbon tetraklorür ile sıçanlarda oluşturulan karaciğer toksisitesi üzerine Amaranthus lividus L. bitkisinin koruyucu etkisi]

Öz:
Objective: Amaranthus lividus is consumed as popular vegetable in West Black Sea Region of Turkey. In this study, we aimed to evaluate the protective and antioxidant effects of A. lividus on carbon tetrachloride (CCl4) induced oxidative stress and acute liver injury in rats.Methods: Male albino Wistar rats were divided into 7 groups: Normal control, A. lividus control, silymarin control, CCl4, A. lividus (250 mg/kg)+CCl4, A. lividus (500 mg/kg)+CCl4, silymarin+CCl4. Rats were orally pretreated with A. lividus (250 and 500 mg/kg) or silymarin (25 mg/kg) daily for 9 days before administration of CCl4 (1.5 mL/kg, 1:1 in olive oil, i.p.).Results: Pretreatment of rats with A. lividus, significantly prevented the CCl4 induced elevation in the levels of serum alanine aminotransferase, aspartate aminotransferase, bilirubin and hepatic lipid peroxidation and myeloperoxidase. In addition, pretreatment with A. lividus significantly prevented the CCl4 induced depletion in the activities of antioxidant enzymes such as catalase, glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase and glutathione level in liver. It has been observed that the hepatoprotective effect of A. lividus was comparable to that of silymarin, a standard drug. Histopathological evaluation of the liver also revealed that A. lividus at 250 mg/kg dose partially suppressed the CCl4 induced liver damage in rats.Conclusion: Our results indicated that A. lividus has a protective effect against CCl4 induced acute hepatotoxicity in rats, and this effect might be related to its antioxidant activity.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA YİLMAZ OZDEN T, CAN A, SANCAR BAŞ S, KARA PALA Z, OKYAR A, BOLKENT Ş (2015). Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. , 125 - 131.
Chicago YİLMAZ OZDEN Tugba,CAN AYSE,SANCAR BAŞ Serap,KARA PALA Zeliha,OKYAR Alper,BOLKENT Şehnaz Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. (2015): 125 - 131.
MLA YİLMAZ OZDEN Tugba,CAN AYSE,SANCAR BAŞ Serap,KARA PALA Zeliha,OKYAR Alper,BOLKENT Şehnaz Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. , 2015, ss.125 - 131.
AMA YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. . 2015; 125 - 131.
Vancouver YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. . 2015; 125 - 131.
IEEE YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş "Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats." , ss.125 - 131, 2015.
ISNAD YİLMAZ OZDEN, Tugba vd. "Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats". (2015), 125-131.
APA YİLMAZ OZDEN T, CAN A, SANCAR BAŞ S, KARA PALA Z, OKYAR A, BOLKENT Ş (2015). Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. Türk Biyokimya Dergisi, 40(2), 125 - 131.
Chicago YİLMAZ OZDEN Tugba,CAN AYSE,SANCAR BAŞ Serap,KARA PALA Zeliha,OKYAR Alper,BOLKENT Şehnaz Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. Türk Biyokimya Dergisi 40, no.2 (2015): 125 - 131.
MLA YİLMAZ OZDEN Tugba,CAN AYSE,SANCAR BAŞ Serap,KARA PALA Zeliha,OKYAR Alper,BOLKENT Şehnaz Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. Türk Biyokimya Dergisi, vol.40, no.2, 2015, ss.125 - 131.
AMA YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. Türk Biyokimya Dergisi. 2015; 40(2): 125 - 131.
Vancouver YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats. Türk Biyokimya Dergisi. 2015; 40(2): 125 - 131.
IEEE YİLMAZ OZDEN T,CAN A,SANCAR BAŞ S,KARA PALA Z,OKYAR A,BOLKENT Ş "Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats." Türk Biyokimya Dergisi, 40, ss.125 - 131, 2015.
ISNAD YİLMAZ OZDEN, Tugba vd. "Protective effect of Amaranthus lividus L. on carbon tetrachloride induced hepatotoxicity in rats". Türk Biyokimya Dergisi 40/2 (2015), 125-131.
Sayfa Oluşturulma Tarihi
22-04-2024 23:59

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