The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)

KOÇ, Evren; ERSAN, Yusuf; ÇITIL, MEHMET; KARAMAN, Musa; ATAKiŞİ, Onur; HARMANKAYA, Ahmet; YILMAZ, MUHİTDİN

The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)

Muhitdin YILMAZ, (Sinop Üniversitesi, Mühendislik ve Mimarlık Fakültesi, Biyomühendislik Bölümü, Sinop, Türkiye)

Evren KOÇ, (Kafkas Üniversitesi, Mühendislik Mimarlık Fakültesi, Biyomühendislik Bölümü, Kars, Türkiye)

Onur ATAKİŞİ, (Kafkas Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Kars, Türkiye)

Ahmet HARMANKAYA, (Kafkas Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Kars, Türkiye)

Yusuf ERSAN, (Kafkas Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kars, Türkiye)

Musa KARAMAN, (Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kars, Türkiye)

Mehmet ÇİTİL (Kafkas Üniversitesi, Veteriner Fakültesi, Anatomi Anabilim Dalı, Kars, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

3 0

Yıl: 2016 Cilt: 22 Sayı: 4 Sayfa Aralığı: 511 - 518 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)

Öz:

Çalışmada, Capoeta capoeta'da Kurşun (II) asetat (Pb(C2H3O2)2) toksisitesine karşı L-karnitinin (LK) koruyucu etkileri histopatolojik, elektroforetik ve biyokimyasal yöntemlerle incelendi. Kars Çayı'ndan yakalanan balıklar 500 L'lik tanklarda her grupta 10'ar adet balık bulunan 4 gruba ayrıldı. Kurşun ve sıvı LK suya ilave edildi. I. gruptaki balıklar kontrol grubu olarak belirlendi. II. gruptaki balıklara 10 gün süreyle 1 mg/L kurşun asetat uygulandı. III. gruptaki balıklara 100 mg/L LK uygulandı. IV. gruptaki balıklar ise 1 mg/L kurşun ve 100 mg/L dozunda LK uygulandı. Karaciğer, solungaç, bağırsak ve böbrek dokularında kurşun asetatın toksik etki gösterdiği ve buna karşı L-karnitin uygulamasının koruyucu etki göstererek oluşan bu dejenerasyonların şiddetini azalttığı gözlemlendi. Elektroforetik incelemede, kurşun asetatın birçok protein bandında inhibisyona neden olduğu, LK uygulaması sonucunda da protein ekspresyonlarında artış meydana geldiği saptandı. Kurşun uygulanan grupta total protein düzeyinin yüksek, kurşun + LK verilen grubun ise düşük olduğu belirlendi (P<0.05). Globulin düzeylerinin, LK + kurşun verilen grupta istatistiksel olarak düşük olduğu saptandı (P<0.05). Kurşun uygulanan grubun Total Oksidan Kapasitesinin (TOK) kontrol grubuna göre yüksek, kurşun + LK verilen grubun TOK düzeyinin ise kontrol ile kurşun verilen grubun arasında olduğu belirlendi. Sonuç olarak; LK'in Capoeta capoeta'da kurşun toksisitesine karşı koruyucu özellik gösterdiği kanısına varıldı.

Anahtar Kelime:

Konular: Veterinerlik

Capoeta capoeta (Guldensteadt 1773)'larda Kurşun (II) Asetat Toksisitesine Karşı L-Karnitinin Koruyucu Etkileri

Öz:

In this study, the protective effects of L-carnitine (LC) against Lead (II) acetate (Pb(C2H3O2)2) toxicity in Capoeta capoeta were investigated by means of histopathologically, via electrophoretic and biochemical methods. Fish caught from Kars creek were divided into four groups, which include 10 fish each in 500 L tanks. Lead and liquid LC were added to water. Fish in the first group were adjusted as control. Fish in the II. group were applied 1 mg/L lead (as Lead (II) acetate) for 10 days. Fish in the III. group were administered 100 mg/L LC. Fish in the IV. group were administered 1 mg/L lead and 100 mg/L LC. Degenerations in liver, gill, intestine and kidney tissues were observed to reduce LC administration against the toxic effects of lead acetate. Electrophoretically, inhibitions of some protein bands in the group, which was applied lead acetate, were caused, and increases in protein expression in the group, which was applied L-carnitine, have occurred. While high a level of total protein in the group that was administered lead was found; in the group that was treated lead + LC, it was found to be lower (P<0.05). Levels of globulin in the group that was administered LC + lead were observed to be significantly lower (P<0.05). Total oxidant capacity (TOC) in lead treatment group were higher than the control group, TOC levels in lead + LC treatment group were determined to be between the control and lead group. LC was concluded to show a protective effect on Capoeta capoeta that were exposed to lead.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YILMAZ M, KOÇ E, ATAKiŞİ O, HARMANKAYA A, ERSAN Y, KARAMAN M, ÇITIL M (2016). The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). , 511 - 518.
Chicago	YILMAZ MUHİTDİN,KOÇ Evren,ATAKiŞİ Onur,HARMANKAYA Ahmet,ERSAN Yusuf,KARAMAN Musa,ÇITIL MEHMET The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). (2016): 511 - 518.
MLA	YILMAZ MUHİTDİN,KOÇ Evren,ATAKiŞİ Onur,HARMANKAYA Ahmet,ERSAN Yusuf,KARAMAN Musa,ÇITIL MEHMET The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). , 2016, ss.511 - 518.
AMA	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). . 2016; 511 - 518.
Vancouver	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). . 2016; 511 - 518.
IEEE	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M "The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)." , ss.511 - 518, 2016.
ISNAD	YILMAZ, MUHİTDİN vd. "The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)". (2016), 511-518.

APA	YILMAZ M, KOÇ E, ATAKiŞİ O, HARMANKAYA A, ERSAN Y, KARAMAN M, ÇITIL M (2016). The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(4), 511 - 518.
Chicago	YILMAZ MUHİTDİN,KOÇ Evren,ATAKiŞİ Onur,HARMANKAYA Ahmet,ERSAN Yusuf,KARAMAN Musa,ÇITIL MEHMET The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22, no.4 (2016): 511 - 518.
MLA	YILMAZ MUHİTDİN,KOÇ Evren,ATAKiŞİ Onur,HARMANKAYA Ahmet,ERSAN Yusuf,KARAMAN Musa,ÇITIL MEHMET The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.22, no.4, 2016, ss.511 - 518.
AMA	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 511 - 518.
Vancouver	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773). Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 511 - 518.
IEEE	YILMAZ M,KOÇ E,ATAKiŞİ O,HARMANKAYA A,ERSAN Y,KARAMAN M,ÇITIL M "The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22, ss.511 - 518, 2016.
ISNAD	YILMAZ, MUHİTDİN vd. "The Protective Effects of L-Carnitine Against Lead (II) Acetate Toxicity in Capoeta capoeta (Guldensteadt 1773)". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22/4 (2016), 511-518.