Role of simvastatin and ROR a activity in the macrophage apoptotic pathway

ÜNALTUNA ERGİNEL, Nihan; Gulec, Cagri; SELÇUK ÖZSAİT, Bilge; coban, neslihan

Role of simvastatin and ROR a activity in the macrophage apoptotic pathway

Neslihan ÇOBAN, (İstanbul Üniversitesi Deneysel Tıp Enstitüsü, Genetik Bölümü, İstanbul, Türkiye)

Çağrı GÜLEÇ, (İstanbul Üniversitesi Deneysel Tıp Enstitüsü, Genetik Bölümü, İstanbul, Türkiye)

Bilge SELÇUK ÖZSAİT, (İstanbul Üniversitesi Deneysel Tıp Enstitüsü, Genetik Bölümü, İstanbul, Türkiye)

Nihan ÜNALTUNA ERGİNEL (İstanbul Üniversitesi Deneysel Tıp Enstitüsü, Genetik Bölümü, İstanbul, Türkiye)

The Anatolian Journal of Cardiology

1 0

Yıl: 2017 Cilt: 17 Sayı: 5 Sayfa Aralığı: 362 - 366 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Role of simvastatin and ROR a activity in the macrophage apoptotic pathway

Öz:

Objective: Atherosclerosis is a chronic inflammatory condition and is one of the main causes of death worldwide. Macrophages play important roles in the formation of atherosclerotic plaques. Apoptosis is progressively observed while plaques develop, although the precise mechanisms and outcomes of apoptosis in atherosclerosis development and progression are still contradictory. This study was conducted to explore the effects of simvastatin and retinoic acid receptor-related orphan receptor alpha (ROR?) ligands on apoptosis in human acute monocytic leukemia (THP-1) macrophage cells.Methods: Briefly, the occupancy of ROR? in the promoter regions of apoptotic pathway genes was demonstrated in THP-1 cell lines using chromatin immunoprecipitation (ChIP) analysis. In order to modulate ROR? activity, THP-1 macrophage cells were treated with specific ligands (CPG52608 and SR1001) and then viability as well as count of THP-1 macrophage cells were analyzed.Results: We observed that simvastatin and both ROR? ligands had a tendency to decrease THP-1 macrophage cell viability in culture. When compared with non-treated controls, simvastatin significantly decreased cell viability (p=0.04) and cell count (p=0.03). However, this negative effect of simvastatin seemed to be partly prevented by ROR? ligands. In addition, bioinformatics analysis of ChIP-on-chip data demonstrated that several genes that are involved in the apoptotic pathway were likely ROR? target genes. These genes were involved in the regulation of apoptosis through various pathways.Conclusion: In summary, our study suggest that simvastatin-mediated macrophage apoptosis might be modulated by SR1001 administration. However, involvement of ROR? in this modulation through potential apoptotic target genes remains elusive.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	coban n, Gulec C, SELÇUK ÖZSAİT B, ÜNALTUNA ERGİNEL N (2017). Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. , 362 - 366.
Chicago	coban neslihan,Gulec Cagri,SELÇUK ÖZSAİT Bilge,ÜNALTUNA ERGİNEL Nihan Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. (2017): 362 - 366.
MLA	coban neslihan,Gulec Cagri,SELÇUK ÖZSAİT Bilge,ÜNALTUNA ERGİNEL Nihan Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. , 2017, ss.362 - 366.
AMA	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. . 2017; 362 - 366.
Vancouver	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. . 2017; 362 - 366.
IEEE	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N "Role of simvastatin and ROR a activity in the macrophage apoptotic pathway." , ss.362 - 366, 2017.
ISNAD	coban, neslihan vd. "Role of simvastatin and ROR a activity in the macrophage apoptotic pathway". (2017), 362-366.

APA	coban n, Gulec C, SELÇUK ÖZSAİT B, ÜNALTUNA ERGİNEL N (2017). Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. The Anatolian Journal of Cardiology, 17(5), 362 - 366.
Chicago	coban neslihan,Gulec Cagri,SELÇUK ÖZSAİT Bilge,ÜNALTUNA ERGİNEL Nihan Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. The Anatolian Journal of Cardiology 17, no.5 (2017): 362 - 366.
MLA	coban neslihan,Gulec Cagri,SELÇUK ÖZSAİT Bilge,ÜNALTUNA ERGİNEL Nihan Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. The Anatolian Journal of Cardiology, vol.17, no.5, 2017, ss.362 - 366.
AMA	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. The Anatolian Journal of Cardiology. 2017; 17(5): 362 - 366.
Vancouver	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N Role of simvastatin and ROR a activity in the macrophage apoptotic pathway. The Anatolian Journal of Cardiology. 2017; 17(5): 362 - 366.
IEEE	coban n,Gulec C,SELÇUK ÖZSAİT B,ÜNALTUNA ERGİNEL N "Role of simvastatin and ROR a activity in the macrophage apoptotic pathway." The Anatolian Journal of Cardiology, 17, ss.362 - 366, 2017.
ISNAD	coban, neslihan vd. "Role of simvastatin and ROR a activity in the macrophage apoptotic pathway". The Anatolian Journal of Cardiology 17/5 (2017), 362-366.