The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities

GEVEN, Fatmagül; SHOMALI MOGHADDAM, Naznoosh; GÜNEŞ, Merve G.; İŞGÖR, Belgin S.; İŞGÖR, Yasemin G.; YILDIRIM, Özlem

The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities

Merve G. GÜNEŞ, (Ankara University, Faculty of Science, Department of Biology, 06100 Ankara, TURKEY,)

Belgin S. İŞGÖR, (Atilim University, Faculty of Engineering, Department of Chemical Engineering and Applied Chemistry, 06836 Ankara, TURKEY,)

Yasemin G. İŞGÖR, (Ankara University, Department of Medicinal Laboratory Techniques, Vocational School of)

Naznoosh SHOMALI MOGHADDAM, (Ankara University, Faculty of Science, Department of Biology, 06100 Ankara, TURKEY,)

Fatmagül GEVEN, (Ankara University, Faculty of Science, Department of Biology, 06100 Ankara, TURKEY,)

Özlem YILDIRIM (Ankara University, Faculty of Science, Department of Biology, 06100 Ankara, TURKEY,)

Turkish Journal of Pharmaceutical Sciences

5 1

Yıl: 2014 Cilt: 11 Sayı: 3 Sayfa Aralığı: 339 - 346 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities

Öz:

Geleneksel Türk tıbbında 'Boğa dikeni' olarak bilinen Eryngium campestre L. (Apiaceae), Türkiye'nin her bölgesinde genişçe yayılım göstermektedir. Bu bitkinin çeşitli kısımlarından hazırlanan infüzyonların, diüretik, iştah açıçı ve uyarıcı olarak etki gösterdiği bilinmektedir. Bu çalışmada, E. campestre'nin çiçek ve yapraklarından elde edilen metanol özütlerinin, antioksidan savunma sistemlerinde yer alan glutatyon- S-transferaz (GST), glutatyon peroksidaz (GPx) ve katalaz (CAT) enzimleri üzerine etkileri incelenmiştir. Elde edilen sonuçlarda, bitkinin çiçek kısımlarının metanol özütlerinin fenolik ve flavonoid içeriklerinin, yapraklarından elde edilen özütlerden daha yüksek değere sahip olduğu bulunmuştur. Biyolojik hedeflere karşı özütlerin etkilerinin değerlendirilmesinde ise, maksimum GST inhibisyonunun, E. campestre'nin çiçek kısmından elde edilen ekstrelerle sağlandığı ve IC50 değerinin 363 ng/mL olduğu görülmüştür. Bu çalışmada değerlendirilen özütlerin GPx ve CAT inhibisyonu sağlamadığı görülmüştür

Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık

Eryngium campestre Ekstrelerinin Glutatyon-S-Transferaz, Glutatyon Peroksidaz ve Katalaz Enzim Aktiviteleri Üzerine Etkileri

Öz:

Eryngium campestre L. (Apiaceae) are known in Turkish folk medicine as ‘Bogadikeni’ and wildly distributed in all parts of Turkey. Infusion of different parts of this species are well known as diuretic, appetizer and stimulant. In the present study, methanol extracts from flowers and leaves of E. campestre were evaluated for their potential medicinal value in terms of biological targets participate in antioxidant defense such as glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT). Results obtained show that the methanol extract of flowers were found to have higher amount of phenolic and flavonoid contents than the leaves. The evaluation of extracts against biological target revealed that, the maximum GST enzymes inhibition activities was observed with methanol extract from flowers of E. campestre with IC50 value of 363 ng/mL. In this study, none of the extracts evaluated were shown any reasonable GPx and CAT inhibition

Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Duke JA, Bogenschutz-Godwin MJ, duCellier J, Duke PAK, Handbook of medicinal herbs, 2nd Ed, pp. 277-278, London, CRC Press, 2002.
2. Erdelmeier CAJ, Sticher O, A cyclohexenone and a cyclohexadienone glycoside from Eryngium campestre, Phytochemistry 25,741- 743, 1986.
3. Wörz A, On the distribution and relationships of the South-West Asian species of Eryngium L. (Apiaceae-Saniculoideae), Turk J Bot 28, 85-92, 2004.
4. Baytop T, Türkiye’de Bitkilerle TedaviGeçmisten Bugüne (Therapy with Medicinal Plants in Turkey-Past and Present) 2nd Ed, pp.169, Nobel Tip Basimevi, Istanbul, 1999.
5. Güner A, Özhatay N, Ekim T, Baser KHC, Flora of Turkey and the East Aegean Islands, Vol 11, pp.136-138, Edinburgh University Press, Edinburgh, 2000.
6. Sezik E, Yesilada E, Tabata M, Honda G, TakaishiY, Fujita T, Tanaka T, Takeda Y, Traditional medicine in Turkey VIII. Folk medicine in East Anatolia; Erzurum, Erzincan, Agri, Kars, Igdir provinces, Eco Bot 51, 195- 211, 1997.
7. Kartal M, Mitaine-Offer AC, Asaker M, Miyamoto T, Calis I, Wagner H, LacailleDubois MA, Two new triterpenesaponins from Eryngium campestre, Chem Pharm Bull 53(10), 1318-1320, 2005.
8. Kartal M, Mitaine-Offer AC, Paululat T, AbuAsaker M, Wagner H, Mirjolet JF, Guilbaud N, Lacaille-Dubois MA, Triterpenesaponins from Eryngium campestre, J Nat Prod 69(7),1105- 1108, 2006.
9. Burg D, Mulder GJ, Glutathione conjugates and their synthetic derivatives as inhibitors of glutathione-dependent enzymes involved in cancer and drug resistance, Drug Metab Rev 34, 821-863, 2002.
10. Nabekura T, Overcoming multidrug resistance in human cancer cells by natural compounds, Toxins 2(6), 1207-1224, 2010.
11. Sheehan D, Meade G, Foley VM, Dowd CA, Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily, Biochem J 360, 1-16, 2001.
12. Potapova O, Haghighi A, Bost F, Liu CT, Birrer MJ, Gjerset R, Mercola D, The Jun kinase stress-activated protein kinase pathway functions to regulate DNA repair and inhibition of the pathway sensitizes tumor cells to cisplatin, J Biol Chem 272(22), 14041- 14044,1997.
13. Tan AC, Konczak I, Sze DMY, Ramzan I,Molecular pathways for cancer chemoprevention by dietary phytochemicals, Nut Cancer Internat J 63(4), 495-505, 2011.
14. Townsend DM, Tew KD, The role of glutathione-S-transferase in anti-cancer drug resistance, Oncogene 22(47), 7369-7375, 2003.
15. Prasad KN, Cole WC, Kumar B, Prasad KC, Pros and cons of antioxidant use during radiation therapy, Cancer Treat Rev 28(2), 79- 91, 2002.
16. Simone CB, Simone NL, Simone V, Simone CB, Antioxidants and other nutrients do not interfere with chemotherapy or radiation therapy and can increase kill and increase survival, Alter Therap Health Med 13(1), 22- 28, 2007.
17. Coruh N, Sagdicoglu Celep AG, Ozgokce F, Iscan M, Antioxidant capacities of Gundelia tourneforii L. extracts and inhibition on glutathione-S-transferase activity, Food Chem 100, 1249-1253, 2007.
18. Slinkard K, Singleton VL, Total phenol analyses: Automation and comparison with manual methods, J Enol Viticul 28, 49-55, 1977.
19. Chang CC, Yang MH, Wen HM, Chern JC, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J Food Drug Anal 10(3), 178-182, 2002.
20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, Protein measurement with the Folin phenol reagent, J Biol Chem 193(1), 265-275, 1951.
21. Habig WH, Pabst MJ, Jakoby WB, Glutathione-S-transferases the first enzymatic step in mercapturic acid formation, J Biol Chem 249, 7130-7139, 1974.
22. Isgor BS, Isgor YG, Effect of alpha-1- adrenoceptor blocker on cytosolic enzyme targets for potential use in cancer chemotherapy, Int J Pharmacol 8(5), 333-343, 2012.
23. Paglia DE, Valentine WN, Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase, J Lab Clin Med 70(1), 158-169, 1967.
24. Yıldırım Ö, Büyükbingöl Z, Effects of supplementation with a combination of cobalt and ascorbic acid on antioxidant enzymes and lipid peroxidation levels in streptozotocindiabetic rat liver, Biological Trace Element Research 90 (1-3), 143-154, 2002.
25. Isgor BS, Isgor YG,Ozalp-Yaman S, The role of metal coordination complexes in cytosolic cellular defense, Pure Appl Chem 85(2), 365- 375, 2013.
26. Bai JZ,Saafi EL, Zhang SP, Cooper GJS, Role of Ca2+ in apoptosis evoked by human amylin in pancreatic islet beta-cells, Biochem J 343, 53-61, 1999.
27. Yıldırım Ö, Aras S, Ergül A, Response of antioxidant system to short-term NaCl stress in rootstock and razaki cultivar, Acta Biol Cracoviensia Series Bot 46, 151-159, 2004.
28. IsgorYG, Iscan M, Ozturk HS, Durak I, Effects of water soluble garlic extract on human leukemia HL60 cell lines, Turk J Biochem 33(3), 78-84, 2008.
29. Fresco P, Borges F, Diniz C, Marques MP, New insights on the anticancer properties of dietary polyphenols, Med Res Rev 26(6), 747- 766, 2006.
30. Huang WY, Cai YZ, Zhang Y, Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention, Nutr Cancer 62(1), 1-20, 2010.
31. Zhang K, Wong KP, Glutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols, Biochem J 325, 417-422, 1997.
32. Gyamfi AM, Ohtani II, Shinno E, Aniya Y, Inhibition of glutathione-S-transferases by thonningianin A, isolated from the African medicinal herb, Thonningia sanguinea in vitro, Food Chem Toxicol 42, 1401-1408, 2004.
33. Nebija F, Stefkov G, Karapandzova M, Stafilov T, KadifkovaPanovska T, Kulevanova S, Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo, Macedon Pharm Bull 55 (1, 2), 22-32, 2009.
34. Kholkhal W, Ilias F, Bekhechi C, AtikBekkara F, Eryngium maritimum: A rich medicinal plant of polyphenols and flavonoids compounds with antioxidant, antibacterial and antifungal activities, Curr Res J Bio Sci 4(4), 437-443, 2012.

APA	GÜNEŞ M, İŞGÖR B, İŞGÖR Y, SHOMALI MOGHADDAM N, GEVEN F, YILDIRIM Ö (2014). The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. , 339 - 346.
Chicago	GÜNEŞ Merve G.,İŞGÖR Belgin S.,İŞGÖR Yasemin G.,SHOMALI MOGHADDAM Naznoosh,GEVEN Fatmagül,YILDIRIM Özlem The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. (2014): 339 - 346.
MLA	GÜNEŞ Merve G.,İŞGÖR Belgin S.,İŞGÖR Yasemin G.,SHOMALI MOGHADDAM Naznoosh,GEVEN Fatmagül,YILDIRIM Özlem The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. , 2014, ss.339 - 346.
AMA	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. . 2014; 339 - 346.
Vancouver	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. . 2014; 339 - 346.
IEEE	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö "The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities." , ss.339 - 346, 2014.
ISNAD	GÜNEŞ, Merve G. vd. "The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities". (2014), 339-346.

APA	GÜNEŞ M, İŞGÖR B, İŞGÖR Y, SHOMALI MOGHADDAM N, GEVEN F, YILDIRIM Ö (2014). The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. Turkish Journal of Pharmaceutical Sciences, 11(3), 339 - 346.
Chicago	GÜNEŞ Merve G.,İŞGÖR Belgin S.,İŞGÖR Yasemin G.,SHOMALI MOGHADDAM Naznoosh,GEVEN Fatmagül,YILDIRIM Özlem The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. Turkish Journal of Pharmaceutical Sciences 11, no.3 (2014): 339 - 346.
MLA	GÜNEŞ Merve G.,İŞGÖR Belgin S.,İŞGÖR Yasemin G.,SHOMALI MOGHADDAM Naznoosh,GEVEN Fatmagül,YILDIRIM Özlem The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. Turkish Journal of Pharmaceutical Sciences, vol.11, no.3, 2014, ss.339 - 346.
AMA	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. Turkish Journal of Pharmaceutical Sciences. 2014; 11(3): 339 - 346.
Vancouver	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities. Turkish Journal of Pharmaceutical Sciences. 2014; 11(3): 339 - 346.
IEEE	GÜNEŞ M,İŞGÖR B,İŞGÖR Y,SHOMALI MOGHADDAM N,GEVEN F,YILDIRIM Ö "The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities." Turkish Journal of Pharmaceutical Sciences, 11, ss.339 - 346, 2014.
ISNAD	GÜNEŞ, Merve G. vd. "The Effects of Eryngium campestre Extracts on Glutathione-S-Transferase, Glutathione Peroxidase and Catalase Enzyme Activities". Turkish Journal of Pharmaceutical Sciences 11/3 (2014), 339-346.