Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats

NAZİFİ, Saeed; HAMIDI, Seyed Ahmadreza; TABATABAEI NAEINI, Aboutorab; TANIDEH, Nader; TABANDEH, Mohammad Reza; ORYAN, Ahmad

Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats

Seyed Ahmadreza HAMIDI, (Shiraz University, Faculty of Veterinary Medicine, Department of Veterinary Surgery, Shiraz, Iran)

Aboutorab TABATABAEI NAEINI, (KIMS Collage branch of Rajiv Gandhi University, Student in Dentistry, Bangalouru, India)

Ahmad ORYAN, (Shiraz University, Faculty of Veterinary Medicine, Department of Pathology, Shiraz, Iran)

Mohammad Reza TABANDEH, (Shiraz University, Faculty of Veterinary Medicine, Department of Biochemistry, Shiraz, Iran)

Nader TANIDEH, (Shiraz University of Medical Sciences, Department of Stem Cell and Transgenic Research Center, Shiraz, Iran)

Saeed NAZİFİ (KIMS Collage branch of Rajiv Gandhi University, Student in Dentistry, Bangalouru, India)

Turkish Journal of Pharmaceutical Sciences

1 0

Yıl: 2017 Cilt: 14 Sayı: 1 Sayfa Aralığı: 65 - 74 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats

Öz:

Amaç: Bu çalışma, sıçanlarda oluşturulan deneysel yara modelinde Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun, enzimatik antioksidanlar üzerindeki etkisini araştırmak amacıyla yapılmıştır. Gereç ve Yöntemler: Genel anestezi altına alınan hayvanlarda, aseptik koşullarda, insizyon yapmak suretiyle kare şeklinde yaralar (2x2 cm) oluşturulmuştur. Daha sonra, hayvanlar rasgele dört gruba ayrılmıştır (I- tedavi edilmemiş kontrol grubu, II- haricen uygulanan baz jel grubu, IIIharicen %5 jel uygulanan grup, IV- haricen %10 jel uygulanan grup). Bulgular: Sonuçlar, Pistacia atlantica yağı ile hazırlanmış jel formülasyonunun haricen uygulanmasının, kontrol ve baz jel gruplarına kıyasla, bütünlüğü bozulmamış stratum bazalisi, olgun granülasyon dokusunu, kıl folikülleri ve ter bezleri ile reepitelizasyonu arttırdığını göstermektedir. Yara oluşumundan 21 gün sonra, %10 yağlı jel ile tedavi edilen hayvanların dokularındaki gerilme kuvveti, kopma gerilimi ve bükülme direnci, kontrol ve baz jel grubu ile karşılaştırıldığında önemli ölçüde artmıştır. Aynı evredeki kontrol ve baz jel ile muamele edilen gruplara kıyasla, kollajen liflerin daha organize bir düzenlenme ve dokunun daha düzgün bir iyileşme gösterdiği belirlenmiştir. Sonuç: Pistacia atlantica yağ jelinin haricen uygulanmasının, sıçanlarda oluşturulan deneysel yaralar üzerinde morfolojik, biyokimyasal ve biyomekanik yönden düzeltici rol oynadığı ortaya koyulmuştur.

Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık

Pistacia atlantica Jel Formülasyonunun Haricen Uygulamasının Sıçanlarda Oluşturulan Deri Yaraları Üzerindeki İyileştirici Etkisi

Öz:

Objectives: This study has been undertaken to investigate the antioxidant activity of the gel formulation from Pistacia atlantica oil extraction onenzymatic antioxidants in experimental wound created in rats.Materials and Methods: Under anesthesia, a square-shaped skin defect (2x2 cm) was created aseptically by surgical incision. Then, animals wererandomly allocated to four groups (I- untreated controls, II- topically treated with base gel, III- topically treated with 5% gel, IV- topically treatedwith 10% gel).Results: The results suggest that topical application of Pistacia atlantica oil gels improved reepithelialization with continuous stratum basalis anda mature granulation tissue and adnexa (hair follicles and sweat gland) compared with control and base gel groups. Treatment with 10% oil gelsignificantly enhanced the tensile strength, ultimate stress, yield strength and stiffness in this group compared with the control and base gel groupsat 21 days post injury. The collagen fibers showed a more organized pattern and the tissue alignment was greater as compared to the control andbase gel-treated groups at the same stage.Conclusion: The results suggest that topical application of Pistacia atlantica oil gel improved the morphological, biochemical and biomechanicalproperties of experimentally-induced wound defects in rats.

Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Lingaraju GM, Krishna V, Joy Hoskeri H, Pradeepa K, Venkatesh, Babu PS. Wound healing promoting activity of stem bark extract of Semecarpus anacardium using rats. Nat Prod Res. 2012;26:2344-2347.
2. Abdel-Rahman HY, Youssef AM. Mastich as an antioxidant. J Am Oil Chem Soc. 1975;52:423-423.
3. Farhoosh R, Khodaparast MHH, Sharif A. Bene hull oil as a highly stable and antioxidative vegetable oil. Eur J Lipid Sci Tech. 2009;111:1259-1265.
4. Daneshrad A, Aynehchi Y. Chemical studies of the oil from pistacia nuts growing wild in Iran. J Am Oil Chem Soc. 1980;57:248-249.
5. Farhoosh R, Tavakoli J, Khodaparast M. Chemical Composition and Oxidative Stability of Kernel Oils from Two Current Subspecies of Pistacia atlantica in Iran. J Am Oil Chem Soc. 2008;85:723-729.
6. Farhoosh R, Hossein M, Tavassoli-Kafrani, Sharif A. Antioxidant activity of the fractions separated from the unsaponifiable matter of bene hull oil. Food Chem. 2011;126:583-589.
7. Sharayei P, Farhoosh R, Poorazrang H, Khodaparast M. Effect of bene kernel oil on the frying stability of canola oil. J Am Oil Chem Soc. 2010;88:647-654.
8. Lampi A M, Kajata L, Kamal-Eldin A, Piironen V. Antioxidant activities of a- and g-tocopherols in the oxidation of rapeseed oil triacrylglycerols. J Am Oil Chem Soc. 1999;76:749-755.
9. Papas A M. Antioxidant status, diet, nutrition and health, CRC Press. Boca Raton FL (USA). 1998.
10. Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair Regen. 2004;12:235-243.
11. Hatanaka E, Curi R. Fatty acids and wound healing: a review. Rev Bras. Farm 2007;88:53-58.
12. Ortonne J, Clevy J. Physiology of cutaneous cicatrization. Rev Prat. 1994;44:1733-1737.
13. Moch D, Sheva T, Heihn H, Schimidt D, Buntroc P. The linoleic acid metabolite 2 Ds-hidroxi-10, 12 (E-Z): octadecadienoic acid is a strong, proinflammatory mediator in a experimental would healing model of the rat. Biochim Biophys Acta. 1990;49:201-207.
14. Clarke SR, Mohamed R, Bian L, Routh AF, Kun JFK, Mond JJ, Tarkowski A, Foster SJ. The Staphylococcus aureus surface protein IsdA mediates resistance to innate defenses of human skin. Cell Host Microbe. 2007;1:199-212.
15. Smith PA, Romesberg FE. Combating bacteria and drug resistance by inhibiting mechanisms of persistence and adaptation. Nat Chem Biol. 2007;3:549-556.
16. Sado Kamdem S, Guerzoni M E, Baranyi J, Pin C. Effect of capric, lauric and alpha-linolenic acids on the division time distributions of single cells of Staphylococcus aureus. Int J Food Microbiol. 2008;128:122-128.
17. de Pablo MA, Cienfuegos GAD. Modulatory effects of dietary lipids on immune system functions. Immunol Cell Biol. 2000;78:31-39.
18. Chen CH, Wang Y, Nakatsuji T. An innate bactericidal oleic acid effective against skin infection of methicillin-resistant Staphylococcus aureus: A therapy concordant with evolutionary medicine. J Microb Biot. 2011;21:391-399.
19. Fernandez-Lopez R, Machon C, Longshaw CM. Unsaturated fatty acids are inhibitors of bacterial conjugation. Microbiology 2005;151:3517-3526.
20. Guo Q, Packer L. Ascorbate dependent recycling of vitamin E homologue trolox by dihydrolipoate and glutathione in murine skin homogenate. Free Radic Biol Med. 2000;29:368-374.
21. Picardo M, Passi S. Skin Immune System. CRC Press, New York, PP, 1997;207-226.
22. Shukla A, Rasik A, Patnaik G. Depletion of reduced glutathione, ascorbic acid, vitamin E and antioxidant defence enzymes in a healing cutaneous wound. Free Radical Res Com. 1997;269:93-101.
23. Hamidi S A, Tabatabaii Naeini A, Tanideh N, Nazifi S. Effects of Pistacia atlantica (subsp. Mutica) oil extract on antioxidant activities during experimentally induced cutaneous wound healing in rats. Veterinary Sci. Dev 2015;5:5672.
24. Martin A. The use of antioxidants in healing. Dermatol Surg. 1996;22:156-160.
25. Niwa Y, Kanoh T, Sakane T, Soh H, Kawai S, Miyachi Y. The ratio of lipid peroxides to superoxide dismutase activity in the skin lesions of patients with severe skin disease: An accurate prognostic indicator. Life Sci. 1987;40:921-927.
26. Silaeva S, Guliaeva N, Khatsernova B, Onufriev M, Nikolaev A. Effects of 4-methyluracil and carnosine on healing of skin wounds in rats. Bull Exp Biol Med. 1990;109:180-182.
27. Slater T. Free radical mechanisms in tissue injury. Biochem J. 1984;222:1-5.
28. Senel O, Cetinkale O, Ozbay G, Ahcioglu F, Bulan R. Oxygen free radicals impair wound healing in ischemic rat skin. Ann Plast Surg. 1997;39:516-523.
29. Schafer M, Werner S. Oxidative stress in normal and impaired wound repair. Pharmacol Res. 2008;58:165-171.
30. Suleyman H, Gumustekin K, Taysi S, Keles S, Oztasan N, Aktas O. Beneficial effects of Hippophae rhamnoides L. on nicotine induced oxidative stress in rat blood compared with vitamin E. Biol Pharm Bull. 2002;25:1133-1136.
31. Baumann LS, Spencer J. The effects of topical vitamin E on the cosmetic appearance of scars. Dermatol Surg. 1999;25:311-315.
32. Rojas AI, Phillips TJ. Patients with chronic leg ulcers show diminished levels of vitamins A and E, carotenes, and zinc. Dermatol Surg. 1999;25:601-604.
33. Varoglu E, Seven B, Gumustekin K, Aktas O, Sahin A, Dane S. The effects of vitamin E and selenium on blood flow to experimental skin burns in rats using the 133Xe clearance technique Cent. Eur J Med Chem. 2010;5:219-223.
34. Farhoosh R, Tavassoli-Kafrani MH. Frying performance of the hull oil unsaponifiable matter of Pistacia atlantica subsp, Mutica. Eur J Lipid Sci Tech. 2010;112:343-348.
35. Oryan A, Tabatabaei Naeini A, Moshiri A, Mohammadalipour A, Tabandeh MR. Modulation of cutaneous wound healing by silymarin in rats. J Wound Care. 2012;21:457-464.
36. Oryan A, Khalafinezad A, Toloo N, Soltani Rad MN. Effects of 4-chloro- 2,6-bis (2-hydroxylbenzyl) phenol on healing of skin wounds and growth of bacteria. J Vet Med. 2007;54:585-591.
37. Oryan A, Moshiri A, Meimandiparizi AH. Effects of sodium-hyaluronate and glucosamine-chondroitin sulfate on remodeling stage of tenotomized superficial digital flexor tendon in rabbits: a clinical, histopathological, ultrastructural, and biomechanical study. Connect Tissue Res. 2011;52:329-339.
38. Oryan A, Moshiri A. A long term study on the role of exogenous human recombinant basic fibroblast growth factor on the superficial digital flexor tendon healing in rabbits. J Musculoskelet Neuronal Interact. 2011;11:185-195.
39. Trudel G, Doherty GP, Koike Y. Restoration of strength despite low stress and abnormal imaging after Achilles injury. Med Sci Sports Exerc. 2009;41:2009-2016.
40. Edwards CA, O'Brien WDJ. Modified assay for determination of hydroxyproline in a tissue hydrolyzate. Clinica Chimica Acta. 1980;104:161-167.
41. Ohkuma S, Shinohara T, Furuhata T. Determination of hexosamines by modified Morgan-Elson reaction. Proc Jpn Acad. 1996;42:970-974.
42. Abramov Y, Golden B, Sullivan M. Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair Regen. 2007;15:80-86.
43. Tanideh N, Masoumi S, Hosseinzadeh M, Safarpour A R, Erjaee H, KoohiHosseinabadi O, Rahimikazerooni S. Healing effect of Pistacia atlantica fruit oil extract in acetic acid-induced colitis in rats. Iran J Med Sci. 2014;39:522-528.
44. Abd Ellah M. Involvement of free radicals in animal diseases. Comparative Clinical Pathology. 2010;19:615-619.
45. Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010;89:219-229.
46. Burgess C. Topical vitamins. J Drugs Dermatol. 2008;7:2-6.
47. Arnold M, Barbul A. Nutrition and wound healing. Plast Reconstr Surg. 2006;117:42-58.
48. Gupta A, Singh R, Raghubir R. Antioxidant status during cutaneous wound healing in immunocompromised rats. Mol Cell Biochem. 2002;241:1-7.
49. Musalmah M, Fairuz A, Gapor M, Wan Ngah W. Effect of palm vitee on wound healing in normal and diabetic rats. Biochem Mol Biol J. 2001;6:34-39.
50. Heyman H, Van De Looverbosch D, Meijer E, Schols J. Benefits of an oral nutritional supplement on pressure ulcer healing in long-term care residents. J Wound Care. 2008;17:476-480.
51. Zampieri N, Zuin V, Burro R, Ottolenghi A, Saverio Camoglio F. A prospective study in children: Pre- and post-surgery use of vitamin E in surgical incisions. J Plast Reconstr Aesthet Surg. 2010;63:1474-1478.
52. Ehrlich H, Tarver H, Hunt T. Inhibitory effects of vitamin E on collagen synthesis and wound repair. Ann Surg. 1972;175:235-240.
53. Erhan V, Bedri S, Kenan G, Omer A, Ali S, Senol D. The effects of vitamin E and selenium on blood flow to experimental skin burns in rats using the 133Xe clearance technique. Cent Eur J Med. 2010;5:219-223.
54. Altavilla D, Saitta A, Cucinotta D. Inhibition of lipid peroxidation restores impaired vascular endothelial growth factor expression and stimulates wound healing and angiogenesis in the genetically diabetic mouse. Diabetes. 2001;50:667-674.
55. Galeano M, Torre V, Deodato B. Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice. Surgery. 2001;129:467-477.
56. Musalmah M, Nizrana M, Fairuz A, NoorAini A, Azian A, Gapor M. Comparative effects of palm vitamin e and alpha-tocopherol on healing and wound tissue antioxidant enzyme levels in diabetic rats. Lipids 2005;40:575-580.
57. Tsoureli-Nikita E, Hercogova J, Lotti T, Menchini G. Evaluation of dietary intake of vitamin E in the treatment of atopic dermatitis: a study of the clinical course and evaluation of the immunoglobulin E serum levels. J Dermatol. 2002;41:146-150.
58. Cabral G. Lipids as bioeffectors in the immune system. Life Sci. 2005;77:1699-1710.
59. Peres C, Otton R, Curi R. Modulation of lymphocyte proliferation by macrophages and macrophages loaded with arachidonic acid. Cell Biochem Biophys. 2005;23:373-381.
60. Declair V. The usefulness of topical application of essential fatty acids (EFA) to prevent pressure ulcers. Ostomy Wound Manag. 1997;43:48-52.
61. Pieper B, Caliri M. Nontraditional wound care: a review of the evidence for the use of sugar, papaya/papain, and fatty acids, J Wound Ostomy Continence Nurs. 2003;30:175-183.
62. Ruthig D, Meckling-Gill K. N-3 and n-6 fatty acids stimulate restitution by independent mechanisms in the IEC-6 model of intestinal wound healing. J Nutr Biochem 2002;13:27-35.
63. Caffrey B, Jonsson HT J. Role of essential fatty acids in cutaneous wound healing in rats. Prog Lipid Res. 1981;20:641-647.
64. Burney D, Goodwin C, Caldwell M, Amoury R. Essential fatty acidy deficiency and impaired wound healing in an infant with gastroschisis. Am Surg. 1979;45:542.
65. Simpson D, Ross R. The neutrophilic leukocyte in wound repair a study with antineutrophil serum. J Clin Invest. 1972;51:2009-2023.
66. Wilgus TA. Immune cells in the healing skin wound: Influential players at each stage of repair. Pharmacol Res. 2008;58:112-116.
67. Dovi J, He L, DiPietro L. Accelerated wound closure in neutrophildepleted mice. J Leukoc Biol. 2003;73:448-455.
68. Pereira LM, Hatanaka E, Martins EF, Oliveira F, Liberti EA, Farsky SH, Curi R, Pithon-Curi TC. Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem Funct. 2008;26:197-204.
69. Cardosoa CR, Oliveiraa LL, Vancima D, Barbana JO, Ferraza GB. Oleic acid modulation of the immune response in wound healing: A new approach for skin repair. Immunobiology. 2011;216:409-415.
70. Jouvenaz G, Nugteren D, Beerthuis R, Van Dorp D. A sensitive method for the determination of prostaglandins by gas chromatography with electron-capture detection. Biochim Biophys Acta. 1970;202:231-234.
71. Calder P. N-3 fatty acids, inflammation, and immunityrelevance to postsurgical and critically ill patients. Lipids. 2004;39:1147-1161.
72. Dirier A, Akmansu M, Bora H. The effect of vitamin E on acute skin reaction caused by radiotherapy. Clin Exp Dermatol. 2007;32:571-573.
73. Panin G, Strumia R, Ursini F. Topical a-tocopherol acetate in the bulk phase. Eight years of experience in skin treatment. Ann N Y Acad Sci. 2004;1031:443-447.
74. Khoosal D, Goldman R. Vitamin E for treating children's scar: does it help reduce scarring? Can Fam Physician. 2006;52:855-856.
75. Greenwald D, Scharzer L, Padawer J, Levenson S, Seifter E. Zone II flexor tendon repair: effects vitamin A, E and beta-carotene. J Surg Res. 1990;49:98-102.
76. Taren D L, Chvapil M, Weber CW. Increasing the breaking strength of wounds exposed to preoperative irradiation using vitamin E supplementation. Int J Vitam Nutr Res. 1987;57:133-137.

APA	HAMIDI S, TABATABAEI NAEINI A, ORYAN A, TABANDEH M, TANIDEH N, NAZİFİ S (2017). Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. , 65 - 74.
Chicago	HAMIDI Seyed Ahmadreza,TABATABAEI NAEINI Aboutorab,ORYAN Ahmad,TABANDEH Mohammad Reza,TANIDEH Nader,NAZİFİ Saeed Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. (2017): 65 - 74.
MLA	HAMIDI Seyed Ahmadreza,TABATABAEI NAEINI Aboutorab,ORYAN Ahmad,TABANDEH Mohammad Reza,TANIDEH Nader,NAZİFİ Saeed Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. , 2017, ss.65 - 74.
AMA	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. . 2017; 65 - 74.
Vancouver	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. . 2017; 65 - 74.
IEEE	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S "Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats." , ss.65 - 74, 2017.
ISNAD	HAMIDI, Seyed Ahmadreza vd. "Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats". (2017), 65-74.

APA	HAMIDI S, TABATABAEI NAEINI A, ORYAN A, TABANDEH M, TANIDEH N, NAZİFİ S (2017). Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turkish Journal of Pharmaceutical Sciences, 14(1), 65 - 74.
Chicago	HAMIDI Seyed Ahmadreza,TABATABAEI NAEINI Aboutorab,ORYAN Ahmad,TABANDEH Mohammad Reza,TANIDEH Nader,NAZİFİ Saeed Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turkish Journal of Pharmaceutical Sciences 14, no.1 (2017): 65 - 74.
MLA	HAMIDI Seyed Ahmadreza,TABATABAEI NAEINI Aboutorab,ORYAN Ahmad,TABANDEH Mohammad Reza,TANIDEH Nader,NAZİFİ Saeed Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turkish Journal of Pharmaceutical Sciences, vol.14, no.1, 2017, ss.65 - 74.
AMA	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turkish Journal of Pharmaceutical Sciences. 2017; 14(1): 65 - 74.
Vancouver	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turkish Journal of Pharmaceutical Sciences. 2017; 14(1): 65 - 74.
IEEE	HAMIDI S,TABATABAEI NAEINI A,ORYAN A,TABANDEH M,TANIDEH N,NAZİFİ S "Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats." Turkish Journal of Pharmaceutical Sciences, 14, ss.65 - 74, 2017.
ISNAD	HAMIDI, Seyed Ahmadreza vd. "Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats". Turkish Journal of Pharmaceutical Sciences 14/1 (2017), 65-74.