Objective: HPLC (High Performance Liquid Chromatography) is the most commonly used method to differentiate the subtypes of hemoglobin in screening for hemoglobinopathy which is a major public health care problem especially in Mediterranean region. Today, different models and trademarks of HPLC equipment have been used in screening for hemoglobinopathy. However, the agreement of the results of the analyses performed by these types of equipment are unknown. In this study, the aim was to investigate the agreement between the results of the analyses of HbA2 values with D-10TM Hemoglobin testing system (BioRad,Hercules,USA) and Tosoh HLC 723 G8 (TosohBioscience, Japan) trademark.Methods: A total of 30 individuals, 12 males and 18 females, applied to the Antalya Training and Research Hospital laboratory between January 1 and January 31, 2016 for thalassemia screening were included to this study. Statistical correlations of hemoglobin A2 (HbA2) values, analyzed in each analyzer as a single analysis from each sample, were evaluated using two-way random intraclass correlation coefficient (ICC), Cohen’s kappa coefficient, correlation coefficient (r) and Bland Altman analysis. Results are presented with graphics.Results: As a result of the analysis, 14 of the samples (46.66%) were detected as – thalassemia carrier with Tosoh HLC 723 G8 system and nine of the samples (30%) were detected as – thalassemia carrier with BioRad D-10TM system. There was significant differance in HbA2 values measured with both systems (z= -4.487p <0.001). For HbA2 values; intraclass correlation coefficient was 0.898, coefficeint of concordance was 0.783, Spearman corelation coefficiency was 0.944, Cohen’s Kappa value 0.658 and Bland Altman analysis bias value was 0.75 (LowerLimit: -0.49; Upper Limit: 1.99). Conclusion: According to the regression, correlation and other statistical analyses, agreement between the results of HbA2 values measured by the two analyzers were found to be insufficient.