Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection

SÜRSAL, Neslihan; KARAER, Zafer; koc, Nafiye; ÇAKMAK, Ayşe; NALBANTOĞLU, AYŞE SERPİL; Orkun, Ömer

Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection

AYŞE SERPİL NALBANTOĞLU, (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY)

Zafer KARAER, (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY)

Ayşe ÇAKMAK, (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY)

Neslihan SÜRSAL, (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY)

Nafiye KOÇ, (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY)

Ömer ORKUN (Department of Parasitology, Faculty of Veterinary Medicine, Ankara University, TR-06110 Diskapi, Ankara - TURKEY ORCID: 0000-0002-4478-2521)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

8 1

Yıl: 2018 Cilt: 24 Sayı: 2 Sayfa Aralığı: 227 - 232 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection

Öz:

Kene kaynaklı kan protozoonları köpek sağlığında önemli bir yer tutmaktadır. Bu protozoonların başında Babesia ve Hepatozoon türleri yer almaktadır. Mevcut türler köpeklerde ılımlı hastalık tablosundan şiddetli enfeksiyona varan derecede klinik belirtilere neden olmaktadırlar. Bu çalışmada, sokak köpeklerindeki kene kaynaklı kan protozoonlarının varlığı mikroskobik ve moleküler olarak araştırılmıştır. İncelenen kan preparatlarının hiçbirinde Babesia spp.'nin piroplazmik formuna rastlanmamıştır. Ancak örneklerin %3.8'inde Hepatozoon spp.'nin gametositleri tespit edilmiştir. Yapılan PCR analizleri sonucunda köpeklerde yüksek oranda (%49.5) Hepatozoon enfeksiyonu tespit edilmiştir. Buna karşın hiçbir köpekte Babesia spp. varlığına rastlanmamıştır. Sekans ve filogenetik analizler sonucunda pozitiflerin %86'sının Hepatozoon canis, %13.7'sinin ise Hepatozoon sp. MF olduğu belirlenmiştir. Bu çalışmada elde edilen pozitiflik oranın, Türkiye'deki birçok bölgeden rapor edilen oranlardan daha yüksek olduğu görülmüştür. Bu durum bölgedeki köpekler için Hepatozoon enfeksiyonu açısından risk teşkil etmektedir. Ayrıca henüz patojenitesi hakkında net bir veri bulunmayan ve yeni bir genotip olan Hepatozoon sp. MF varlığı da bölgedeki köpeklerden bildirilmiş olup bu genotip ile ilgili detaylı patojenite ve epidemiyolojik çalışmalara ihtiyaç duyulduğu görülmüştür. Sonuç olarak Ankara'daki sokak köpeklerinde H. canis'in yaygın olarak bulunduğu ortaya konmuştur. Bu nedenle, kliniklerde hepatozoonosis enfeksiyonları dikkate alınmalı ve ayırıcı tanıda göz ardı edilmemelidir

Anahtar Kelime:

Konular: Veterinerlik

İç Anadolu Bölgesindeki Sokak Köpeklerinde Kene-Kaynaklı Kan Protozoonları Varlığının Araştırılması ve Yüksek Orandaki Hepatozoon Enfeksiyonunun Moleküler Karakterizasyonu

Öz:

Tick-borne blood protozoa play an important role in canine health, especially, Babesia and Hepatozoon species. In dogs, these species lead to clinical symptoms ranging from mild to severe. This study aimed to investigate the presence of tick-borne blood protozoa in stray dogs using microscopy and molecular methods. While none of the blood smears showed any Babesia spp. piroplasms, Hepatozoon spp. gametocytes were detected in 3.8% of examined samples. PCR analyses revealed the presence of a Hepatozoon infection in 49.5% of dogs. However, the presence of Babesia spp. was not found in any dogs. Sequence and phylogenetic analyses revealed that 86% of the positive cases were Hepatozoon canis and 13.7% were Hepatozoon sp. MF. The positivity rate obtained in this study was higher than the reported rates in many regions in Turkey. This suggests that Hepatozoon infections present a risk to dog health in this region. In addition, Hepatozoon sp. MF (a new genotype of undetermined pathogenicity) is reported in dogs from this region and detailed pathogenicity and epidemiological studies are thus required for this genotype. This study, therefore, suggests that H. canis is common in stray dogs in Ankara. Canine hepatozoonosis should be taken into consideration in pet clinics and the differential diagnosis should not be overlooked

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NALBANTOĞLU A, KARAER Z, ÇAKMAK A, SÜRSAL N, koc N, Orkun Ö (2018). Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. , 227 - 232.
Chicago	NALBANTOĞLU AYŞE SERPİL,KARAER Zafer,ÇAKMAK Ayşe,SÜRSAL Neslihan,koc Nafiye,Orkun Ömer Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. (2018): 227 - 232.
MLA	NALBANTOĞLU AYŞE SERPİL,KARAER Zafer,ÇAKMAK Ayşe,SÜRSAL Neslihan,koc Nafiye,Orkun Ömer Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. , 2018, ss.227 - 232.
AMA	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. . 2018; 227 - 232.
Vancouver	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. . 2018; 227 - 232.
IEEE	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö "Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection." , ss.227 - 232, 2018.
ISNAD	NALBANTOĞLU, AYŞE SERPİL vd. "Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection". (2018), 227-232.

APA	NALBANTOĞLU A, KARAER Z, ÇAKMAK A, SÜRSAL N, koc N, Orkun Ö (2018). Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24(2), 227 - 232.
Chicago	NALBANTOĞLU AYŞE SERPİL,KARAER Zafer,ÇAKMAK Ayşe,SÜRSAL Neslihan,koc Nafiye,Orkun Ömer Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 24, no.2 (2018): 227 - 232.
MLA	NALBANTOĞLU AYŞE SERPİL,KARAER Zafer,ÇAKMAK Ayşe,SÜRSAL Neslihan,koc Nafiye,Orkun Ömer Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.24, no.2, 2018, ss.227 - 232.
AMA	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2018; 24(2): 227 - 232.
Vancouver	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2018; 24(2): 227 - 232.
IEEE	NALBANTOĞLU A,KARAER Z,ÇAKMAK A,SÜRSAL N,koc N,Orkun Ö "Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24, ss.227 - 232, 2018.
ISNAD	NALBANTOĞLU, AYŞE SERPİL vd. "Molecular Characterization of Tick-Borne Blood Protozoa in Stray Dogs from Central Anatolia Region of Turkey with a High-Rate Hepatozoon Infection". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 24/2 (2018), 227-232.