Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis

Leyla ACAR, (Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey)
NAZAN ATALAN ÖZLEN, (Clinic of Anesthesia and Reanimation, Siyami Ersek Thoracic Cardiovascular Surgery Training and Research Hospital, İstanbul, Turkey)
E Hande KARAGEDİK, (Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey)
Arzu ERGEN (Department of Molecular Medicine, İstanbul University Institute of Experimental Medicine, İstanbul, Turkey)
Balkan Medical Journal
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Yıl: 2018 Cilt: 35 Sayı: 1 Sayfa Aralığı: 30 - 35 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis

Öz:
Background: The humoral system is activated andvarious cytokines are released due to infections intissues and traumatic damage. Nuclear factor-kappa Bdimers are encoded by nuclear factor-kappa B genesand regulate transcription of several crucial proteins ofinflammation such as tumour necrosis factor-alpha.Aims: To investigate the possible effect ofpolymorphisms on tumour necrosis factor-alpha serumlevels with clinical and prognostic parameters of sepsisby determining the nuclear factor-kappa B-1-94 ins/del ATTG and tumour necrosis factor-alpha (-308 G/A)gene polymorphisms and tumour necrosis factor-alphaserum levels.Study Design: Case-control study.Methods: Seventy-two patients with sepsis and 104healthy controls were included in the study. In order todetermine the polymorphisms of nuclear factor-kappaB-1-94 ins/del ATTG and tumour necrosis factor-alpha(-308 G/A), polymerase chain reaction–restrictionfragment length polymorphism analysis was performedand serum tumour necrosis factor-alpha levels weredetermined using an enzyme-linked immunosorbentassay.Results: We observed no significant differences intumour necrosis factor-alpha serum levels between thestudy groups. In the patient group, an increase in thetumour necrosis factor-alpha serum levels in patientscarrying the tumour necrosis factor-alpha (-308 G/A) Aallele compared to those without the A allele was foundto be statistically significant. Additionally, an increase inthe tumour necrosis factor-alpha serum levels in patientscarrying tumour necrosis factor-alpha (-308 G/A) AAgenotype compared with patients carrying the AG or GGgenotypes was statistically significant. No significantdifferences were found in these 2 polymorphismsbetween the patient and control groups (p>0.05).Conclusion: Our results showed the AA genotype andthe A allele of the tumour necrosis factor-alpha (-308G/A) polymorphism may be used as a predictor ofelevated tumour necrosis factor-alpha levels in patientswith sepsis.
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  • Hotchkiss RS, Karl IE. The Pathophysiology and treatment of sepsis. N Engl J Med 2003;348:138-50.
  • Karban AS, Okazaki T, Panhuysen CI, Gallegos T, Potter JJ, Bailey-Wilson JE, et al. Functional annotation of a novel NFKB1 promoter polymorphism that increases risk for ulcerative colitis. Hum Mol Genet 2004;13:35-45.
  • Perkins ND. Integrating cell-signalling pathways with NF-kappaB and IKK function. Nat Rev Mol Cell Biol 2007;8:49-62.
  • Chen F, Castranova V, Shi X, Demers LM. New insights into the role of nuclear factor-kappaB, a ubiquitous transcription factor in the initiation of diseases. Clin Chem 1999;45:7-17.
  • Schäfer ST, Gessner S, Scherag A, Rump K, Frey UH, Siffert W, et al. Hydrocortisone fails to abolish NF-κB1 protein nuclear translocation in deletion allele carriers of the NFKB1 promoter polymorphism (-94ins/ delATTG) and is associated with increased 30-day mortality in septic shock. PLoS One 2014;9:e104953.
  • Reinhart K, Karzai W. Anti-tumor necrosis factor therapy in sepsis: update on clinical trials and lessons learned. Crit Care Med 2001;29:121-5.
  • Feezor RJ, Oberholzer C, Baker HV, Novick D, Rubinstein M, Moldawer LL, et al. Molecular characterization of the acute inflammatory response to infections with gram-negative versus gram-positive bacteria. Infect Immun 2003;5803-13.
  • Pujhari SK, Ratho RK, Prabhakar S, Mishra B, Modi M. TNF-α promoter polymorphism: a factor contributing to the different immunological and clinical phenotypes in Japanese encephalitis. BMC Infect Dis 2012;12:23.
  • Hillenbrand A, Knippschild U, Weiss M, Schrezenmeier H, Henne- Bruns D, Huber-Lang M, et al. Sepsis induced changes of adipokines and cytokines - septic patients compared to morbidly obese patients. BMC Surg 2010;10:26.
  • Mira JP, Cariou A, Grall F, Delclaux C, Losser MR, Heshmati F, et al. Association of TNF2, a TNF-alpha promoter polymorphism, with septic shock susceptibility and mortality: a multicenter study. JAMA 1999;282:561-8.
  • Appoloni O, Dupont E, Vandercruys M, Andriens M, Duchateau J, Vincent JL. Association of tumor necrosis factor-2 allele with plasma tumor necrosis factor-alpha levels and mortality from septic shock. Am J Med 2001;110:486-488.
  • Louis E, Franchimont D, Piron A, Gevaert Y, Schaaf-Lafontaine N, Roland S, et al. Tumour necrosis factor (TNF) gene polymorphism influences TNF-alpha production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans. Clin Exp Immunol 1998;113:401- 6.
  • Zhang M, Zhao Y, Liu Q. Tumor necrosis factor-α -308G/A and -238G/A polymorphisms are associated with increased risks of sepsis: evidence from an updated meta-analysis. APMIS 2017;125:459-67.
  • Bone RC, Balk RA, Cerra FB, Dellinger RP, Fein AM, Knaus WA, et al. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine. Chest 1992;101:1644-55.
  • Goldstein B, Giroir B, Randolph A; International Consensus Conference on Pediatric Sepsis. International pediatric sepsis consensus conference: definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med 2005;6:2-8.
  • Bernard GR, Vincent JL, Laterre PF, LaRosa SP, Dhainaut JF, Lopez- Rodriguez A, et al. Efficacy and safety of recombinant human activated protein C for severe sepsis. N Eng J Med 2001;344:699-709.
  • Opal SM, DePalo VA. Anti-inflammatory cytokines. Chest 2000;117:1162- 72.
  • O'Dwyer MJ, Mankan AK, Stordeur P, O'Connell B, Duggan E, White M. The occurrence of severe sepsis and septic shock are related to distinct patterns of cytokine gene expression. Shock 2006;26:544-50.
  • Srinivasan L, Page G, Kirpalani H, Murray JC, Das A, Higgins RD, et al. Genome-wide association study of sepsis in extremely premature infants. Arch Dis Child Fetal Neonatal Ed 2017;102:439-45.
  • Somech R, Amariglio N, Spirer Z, Rechavi G. Genetic predisposition to infectious pathogens: a review of less familiar variants. Pediatr Infect Dis J 2003;22:457-61.
  • Emonts M, Hazelzet JA, de Groot R, Hermans PW. Host genetic determinants of Neisseria meningitidis infections. Lancet Infect Dis 2003;3:565-77.
  • Tak PP, Firestein GS. NF-kappaB: a key role in inflammatory diseases. J Clin Invest 2001;107:7-11.
  • Gökay SS, Yıldızdaş RD, Yılmaz M, Aksoy K, Yalın AE, Sertdemir Y, et al. MyD 88 Polymorphisms in Children Diagnosed with Sepsis. Balkan Med J 2016;33:633-8.
  • Giachelia M, Voso MT, Tisi MC, Martini M, Bozzoli V, Massini G, et al. Interleukin-6 plasma levels are modulated by a polymorphism in the NF- κB1 gene and are associated with outcome following rituximab-combined chemotherapy in diffuse large B-cell non-Hodgkin lymphoma. Leuk Lymphoma 2012;53:411-6.
  • Barber RC, Aragaki CC, Rivera-Chavez FA, Purdue GF, Hunt JL, Horton JW. TLR4 and TNF-alpha polymorphisms are associated with an increased risk for severe sepsis following burn injury. J Med Genet 2004;41:808-13.
  • Song Z, Song Y, Yin J, Shen Y, Yao C, Sun Z, et al. Genetic variation in the TNF gene is associated with susceptibility to severe sepsis, but not with mortality. PLoS One 2012;7:46113.
  • Feng B, Mao ZR, Pang K, Zhang SL, Li L. Association of tumor necrosis factor α -308G/A and interleukin-6 -174G/C gene polymorphism with pneumonia-induced sepsis. J Crit Care 2015;30:920-3.
  • Baghel K, Srivastava RN, Chandra A, Goel SK, Agrawal J, Kazmi HR, et al. TNF-α, IL-6, and IL-8 cytokines and their association with TNF-α-308 G/A polymorphism and postoperative sepsis. J Gastrointest Surg 2014;18:1486-94.
  • de Bont ES, Martens A, van Raan J, Samson G, Fetter WP, Okken A, et al. Tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-6 plasma levels in neonatal sepsis. Pediatr Res 1993;33:380-3.
  • Ozdemir A, Oygür N, Gültekin M, Coşkun M, Yeğin O. Neonatal tumor necrosis factor, interleukin-1 alpha, interleukin-1 beta, and interleukin-6 response to infection. Am J Perinatol 1994;11:282-5.
  • Casey LC, Balk RA, Bone RC. Plasma cytokine and endotoxin levels correlate with survival in patients with the sepsis syndrome. Ann Intern Med 1993;119:771-8.
  • van Deuren M, van der Ven-Jongekrijg J, Demacker PN, Bartelink AK, van Dalen R, Sauerwein RW, et al. Differential expression of proinflammatory cytokines and their inhibitors during the course of meningococcal infections. J Infect Dis 1994;169:157-61.
  • Kothari N, Bogra J, Abbas H, Kohli M, Malik A, Kothari D, et al. Tumor necrosis factor gene polymorphism results in high TNF level in sepsis and septic shock. Cytokine 2013;61:676-81.
  • Bavunoglu I, Genc H, Konukoglu D, Cicekci H, Sozer V, Gelisgen R, et al. Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis. J Infect Dev Ctries 2016;10:1045-52.
  • Butt C, Sun S, Peddle L, Greenwood C, Hamilton S, Gladman D, et al. Association of nuclear factor-kappaB in psoriatic arthritis. J Rheumatol 2005;32:1742-4.
  • Borm ME, van Bodegraven AA, Mulder CJ, Kraal G, Bouma G. A NFKB1 promoter polymorphism is involved in susceptibility to ulcerative colitis. Int J Immunogenet 2005;32:401-5.
  • Adamzik M, Schäfer S, Frey UH, Becker A, Kreuzer M, Winning S, et al. The NFKB1 promoter polymorphism (-94ins/delATTG) alters nuclear translocation of NF-κB1 in monocytes after lipopolysaccharide stimulation and is associated with increased mortality in sepsis. Anesthesiology 2013;118:123-33.
APA ACAR L, ATALAN ÖZLEN N, KARAGEDİK E, ERGEN A (2018). Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. , 30 - 35.
Chicago ACAR Leyla,ATALAN ÖZLEN NAZAN,KARAGEDİK E Hande,ERGEN Arzu Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. (2018): 30 - 35.
MLA ACAR Leyla,ATALAN ÖZLEN NAZAN,KARAGEDİK E Hande,ERGEN Arzu Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. , 2018, ss.30 - 35.
AMA ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. . 2018; 30 - 35.
Vancouver ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. . 2018; 30 - 35.
IEEE ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A "Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis." , ss.30 - 35, 2018.
ISNAD ACAR, Leyla vd. "Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis". (2018), 30-35.
APA ACAR L, ATALAN ÖZLEN N, KARAGEDİK E, ERGEN A (2018). Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal, 35(1), 30 - 35.
Chicago ACAR Leyla,ATALAN ÖZLEN NAZAN,KARAGEDİK E Hande,ERGEN Arzu Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal 35, no.1 (2018): 30 - 35.
MLA ACAR Leyla,ATALAN ÖZLEN NAZAN,KARAGEDİK E Hande,ERGEN Arzu Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal, vol.35, no.1, 2018, ss.30 - 35.
AMA ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal. 2018; 35(1): 30 - 35.
Vancouver ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis. Balkan Medical Journal. 2018; 35(1): 30 - 35.
IEEE ACAR L,ATALAN ÖZLEN N,KARAGEDİK E,ERGEN A "Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis." Balkan Medical Journal, 35, ss.30 - 35, 2018.
ISNAD ACAR, Leyla vd. "Tumour Necrosis Factor-alpha and Nuclear Factor-kappa B Gene Variants in Sepsis". Balkan Medical Journal 35/1 (2018), 30-35.
Sayfa Oluşturulma Tarihi
03-05-2024 13:43

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