Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)

ALTUNTAS, HULYA; DUMAN ERBAŞ, EMİNE

Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)

EMİNE DUMAN ERBAŞ, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Hülya ALTUNTAŞ (Eskişehir Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Biological Diversity and Conservation

5 1

Yıl: 2018 Cilt: 11 Sayı: 3 Sayfa Aralığı: 24 - 30 Metin Dili: İngilizce İndeks Tarihi: 26-06-2019

Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)

Öz:

In this paper, genotoxic effects of the pure azadirachtin (AZA) on the larval hemocytes of model insect andstorage pest Galleria mellonella L. (Lepidoptera: Pyralidae) was investigated. The comet assay was performed to measureand analyze DNA damage in larval hemocytes at Anadolu University (Eskisehir Technical University) between 2017and 2018 years. For this purpose, sublethal AZA doses (0.5, 1, 1.5, and 2 μg/larva) given to G. mellonella larvae via insectforce feeding method were used to monitor tail intensity, tail moment and tail migration the commonly known cometparameters. These DNA damage indicators were analyzed in hemocytes obtained from larvae at 24 and 72 h post forcefeeding. All comet parameters at all doses of AZA increased in comparison with negative and positive control at 24 and72 h. At 72 h post force feeding with median lethal dose of AZA, a significant increase in DNA damage indicators wasobserved in larval hemocytes as compared with untreated groups. Consequently, this study showed that AZA causedsignificant damage in the genome of G. mellonella larvae even at sublethal doses and the comet assay was useful in themonitoring of in vivo genotoxicity of AZA in larva hemocytes.

Anahtar Kelime:

Azadirachtin’in Galleria mellonella (Lepidoptera: Pyralidae) üzerindeki genotoksisitesi

Öz:

Bu çalışmada, azadirachtin (AZA)’in model böcek ve depo zararlısı olan Galleria mellonella L. (Lepidoptera: Pyralidae)’nın larval hemositleri üzerindeki genotoksik etkileri araştırılmıştır. Larval hemositlerdeki DNA hasarının analiz edilmesi ve ölçülmesi comet analizi kullanılarak, 2017-2018 yılları arasında Anadolu Üniversitesi (Eskişehir Teknik Üniversitesi)’nde gerçekleştilmiştir. Bu amaçla, hemositlerdeki kuyruk yoğunluğu, kuyruk momenti ve kuyruk göçü gibi önemli comet parametrelerinin analiz edilmesi için G. mellonella larvalarına, böcekler için ağızdan besleme yöntemiyle verilen subletal AZA dozları (0.5, 1, 1.5 ve 2 μg/larva) kullanılmıştır. Ağızdan besleme uygulamasından 24 ve 72 saat sonra larvalardan elde edilen hemositlerde bu DNA indikatörleri analiz edilmiştir. AZA uygulanmasından 24 ve 72 saat sonra, larval hemositlerde belirlenen tüm comet parametrelerinde negatif ve pozitif kontrol grubuna kıyasla hem doza hem de zamana bağlı olarak artış meydana geldiği görülmüştür. Özellikle, ortalama letal doz AZA uygulamasından 72 saat sonra, larval hemositlerdeki DNA hasarı indikatörlerinde kontrol gruplarına kıyasla önemli bir artış gözlenmiştir. Sonuç olarak, bu çalışma AZA’nın subletal dozlarda bile G. mellonella larval genomunda önemli oranda hasara neden olduğunu ve larval hemositlerinde AZA’nın genotoksisitesinin in vivo değerlendirilmesinde comet analizinin kullanışlı olduğunu göstermiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DUMAN ERBAŞ E, ALTUNTAS H (2018). Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). , 24 - 30.
Chicago	DUMAN ERBAŞ EMİNE,ALTUNTAS HULYA Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). (2018): 24 - 30.
MLA	DUMAN ERBAŞ EMİNE,ALTUNTAS HULYA Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). , 2018, ss.24 - 30.
AMA	DUMAN ERBAŞ E,ALTUNTAS H Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). . 2018; 24 - 30.
Vancouver	DUMAN ERBAŞ E,ALTUNTAS H Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). . 2018; 24 - 30.
IEEE	DUMAN ERBAŞ E,ALTUNTAS H "Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)." , ss.24 - 30, 2018.
ISNAD	DUMAN ERBAŞ, EMİNE - ALTUNTAS, HULYA. "Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)". (2018), 24-30.

APA	DUMAN ERBAŞ E, ALTUNTAS H (2018). Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation, 11(3), 24 - 30.
Chicago	DUMAN ERBAŞ EMİNE,ALTUNTAS HULYA Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation 11, no.3 (2018): 24 - 30.
MLA	DUMAN ERBAŞ EMİNE,ALTUNTAS HULYA Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation, vol.11, no.3, 2018, ss.24 - 30.
AMA	DUMAN ERBAŞ E,ALTUNTAS H Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation. 2018; 11(3): 24 - 30.
Vancouver	DUMAN ERBAŞ E,ALTUNTAS H Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae). Biological Diversity and Conservation. 2018; 11(3): 24 - 30.
IEEE	DUMAN ERBAŞ E,ALTUNTAS H "Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)." Biological Diversity and Conservation, 11, ss.24 - 30, 2018.
ISNAD	DUMAN ERBAŞ, EMİNE - ALTUNTAS, HULYA. "Genotoxicity of azadirachtin on Galleria mellonella L. (Lepidoptera: Pyralidae)". Biological Diversity and Conservation 11/3 (2018), 24-30.