Objective: The purpose of this study was to evaluate the frequen- cy and severity of symptoms experienced in patients undergoing hemodialysis. Methods: A cross-sectional and descriptive study including 194 patients was conducted between May and November 2015. Data were collected using a face-to-face interview method with “patient information form” and “dialysis symptom index.” The number, percentage, mean, standard deviation, t-test, Mann– Whitney U test, and one-way analysis of variance test were used to evaluate the data. Results: Most patients undergoing hemodialysis experience symptoms such as tiredness/a lack of energy (83.5%; 3.77±1.03), muscle cramps (74.7%; 3.19±0.90), and bone/joint pain (73.7%; 3.27±0.96). Gender, marital status, educational level, working status and occupation, another chronic disease accompanying a chronic kidney disease, hemodialysis access route, additional he- modialysis in the past month, and erythropoietin use negatively affected the dialysis symptom index point average (p<0.05). Conclusion: The frequency and severity of the symptoms expe- rienced by hemodialysis patients should be regularly monitored, and there should be an organized training on chronic renal dis- ease and hemodialysis treatment in these patients.