Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect
Yıl: 2019 Cilt: 46 Sayı: 1 Sayfa Aralığı: 125 - 132 Metin Dili: İngilizce DOI: 10.5798/dicletip.534849 İndeks Tarihi: 25-10-2019
Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect
Öz: Objective: The effect of lipoic acid (LA), which is considered to be a potential antioxidant, on oxidative stressparameters in liver tissue damage has not been sufficiently investigated. Therefore, we aimed to investigate therelationship of LA with oxidant and antioxidant parameters in lipopolysaccharide (LPS) induced liver damage in rats.Methods: First group (n=10) was injected a single dose of 20 mg/kg saline (control), second group (n=10) wasinjected a single dose of 20 mg/kg LPS (LPS) and third group (n=10) was injected a single dose of 20 mg/kg LPS at theend of third day of 10 mg/kg/day LA injection (LA+LPS). The glutathione peroxidase (GPX), catalase (CAT) andsuperoxide dismutase (SOD) activities and the levels of malondialdehyde (MDA) were determined byspectrophotometric method. 3-Nitrotyrosine (3-NT) level was measured by high performance liquid chromatography. Results: It was found that 3-NT and MDA levels were significantly lower in LA+LPS group than those of LPS group.Moreover, it was found that antioxidant enzyme activity values of LA+LPS approached to the values of control group.Conclusion: These findings indicate that the oxidant/antioxidant status is balanced in the LA group. Therefore, it maybe suggested that LA supplementation to be beneficial for preventing oxidative stress in LPS-induced liver damage inrats.
Anahtar Kelime: Konular:
Alfa-lipoik asit, antioksidan etkisi ile sıçanlarda lipopolisakkarit kaynaklı karaciğer hasarını azaltır
Öz: Amaç: Potansiyel bir antioksidan olarak kabul edilen lipoik asidin (LA), karaciğer doku hasarında oksidatif stres parametreleri üzerindeki etkisi yeterince incelenmemiştir. Bu nedenle, sıçanlarda lipopolisakkaridle (LPS) indüklenen karaciğer hasarında LA'nın oksidan ve antioksidan parametrelerle ilişkisini incelemeyi amaçladık. Yöntemler: Birinci gruba (n=10), 20 mg/kg tek doz salin enjekte edildi (kontrol), ikinci gruba (n=10), tek doz 20 mg/kg LPS enjekte edildi (LPS) ve üçüncü gruba (n=10) 3 gün boyunca enjekte edilen 10 mg/kg LA sonrasında tek doz 20 mg/kg LPS enjekte edildi (LA+LPS). Glutatyon peroksidaz (GPX), katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ve malondialdehid (MDA) düzeyleri spektrofotometrik yöntemle belirlendi. 3-Nitrotirosin (3-NT) seviyesi, yüksek performanslı sıvı kromatografisi ile ölçüldü. Bulgular: LA+LPS grubunda 3-NT ve MDA düzeylerinin LPS grubuna göre anlamlı derecede düşük olduğu bulundu. Ayrıca, LA+LPS'nin antioksidan enzim aktivite değerlerinin kontrol grubu değerlerine yaklaştığı bulundu. Sonuç: Bu bulgular, LA grubunda oksidan/antioksidan durumunun dengelendiğini göstermektedir. Bundan dolayı, LA takviyesinin, sıçanlarda LPS kaynaklı karaciğer hasarındaki oksidatif stresi önlemede faydalı olduğu öne sürülebilir
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Çimen B, Çimen L, ÇETİN İ, CETİNYUREK--YAVUZ A (2019). Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. , 125 - 132. 10.5798/dicletip.534849 |
| Chicago | Çimen Behzat,Çimen Leyla,ÇETİN İHSAN,CETİNYUREK--YAVUZ Aysun Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. (2019): 125 - 132. 10.5798/dicletip.534849 |
| MLA | Çimen Behzat,Çimen Leyla,ÇETİN İHSAN,CETİNYUREK--YAVUZ Aysun Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. , 2019, ss.125 - 132. 10.5798/dicletip.534849 |
| AMA | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. . 2019; 125 - 132. 10.5798/dicletip.534849 |
| Vancouver | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. . 2019; 125 - 132. 10.5798/dicletip.534849 |
| IEEE | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A "Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect." , ss.125 - 132, 2019. 10.5798/dicletip.534849 |
| ISNAD | Çimen, Behzat vd. "Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect". (2019), 125-132. https://doi.org/10.5798/dicletip.534849 |
| APA | Çimen B, Çimen L, ÇETİN İ, CETİNYUREK--YAVUZ A (2019). Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. Dicle Tıp Dergisi, 46(1), 125 - 132. 10.5798/dicletip.534849 |
| Chicago | Çimen Behzat,Çimen Leyla,ÇETİN İHSAN,CETİNYUREK--YAVUZ Aysun Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. Dicle Tıp Dergisi 46, no.1 (2019): 125 - 132. 10.5798/dicletip.534849 |
| MLA | Çimen Behzat,Çimen Leyla,ÇETİN İHSAN,CETİNYUREK--YAVUZ Aysun Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. Dicle Tıp Dergisi, vol.46, no.1, 2019, ss.125 - 132. 10.5798/dicletip.534849 |
| AMA | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. Dicle Tıp Dergisi. 2019; 46(1): 125 - 132. 10.5798/dicletip.534849 |
| Vancouver | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect. Dicle Tıp Dergisi. 2019; 46(1): 125 - 132. 10.5798/dicletip.534849 |
| IEEE | Çimen B,Çimen L,ÇETİN İ,CETİNYUREK--YAVUZ A "Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect." Dicle Tıp Dergisi, 46, ss.125 - 132, 2019. 10.5798/dicletip.534849 |
| ISNAD | Çimen, Behzat vd. "Alpha-lipoic acid alleviates lipopolysaccharide-induced liver damage in rats via antioxidant effect". Dicle Tıp Dergisi 46/1 (2019), 125-132. https://doi.org/10.5798/dicletip.534849 |
