The role of ADAMTS genes in the end stage of hip osteoarthritis

Ataoğlu, Baybars; DEMİRCAN, Kadir; Atalar, Hakan; Turanlı, Sacit; Esen, Erdinç; AYANOĞLU, Tacettin

The role of ADAMTS genes in the end stage of hip osteoarthritis

Tacettin AYANOĞLU, (Yozgat Şehir Hastanesi)

Hakan ATALAR, (Gazi Üniversitesi)

Erdinç ESEN, (Gazi Üniversitesi)

Muhammet Baybars ATAOĞLU, (Gazi Üniversitesi)

Sacit TURANLI, (Gazi Üniversitesi)

Kadir DEMİRCAN (Gazi Üniversitesi)

Acta Orthopaedica et Traumatologica Turcica

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Yıl: 2019 Cilt: 53 Sayı: 2 Sayfa Aralığı: 140 - 144 Metin Dili: İngilizce İndeks Tarihi: 09-12-2019

The role of ADAMTS genes in the end stage of hip osteoarthritis

Öz:

Purpose: The aim of this study is to investigate which ADAMTS genes play a major role in the development of primary hip osteoarthritis, by comparing the tissue and blood samples in patients with hiposteoarthritis and a control group.Material and methods: Human articular cartilage was obtained from femoral heads of 15 patients withend stage osteoarthritis undergoing total hip replacement. As the control group, the cartilages wasobtained from femoral heads of 15 patients, who did not have osteoarthritis or degenerative changes inhip joint, undergoing hip replacement following the fracture of the femoral neck. After the cartilagesamples were taken from the resection materials, the DNA polymorphisms in the patients' cartilagesamples were tested by Polymerase Chain Reaction (PCR), the serum levels of aggrecanase genes wereanalyzed with Enzyme-Linked ImmunoSorbent Assay (ELISA).Results: The level of ADAMTS5 and ADAMTS9 genes were found significantly lower as a result of ELISAanalysis degenerative arthritis group than the control group (p < 0,05). ADAMTS 1, 4, 8, 15 were similarbetween the two groups in ELISA analysis (p > 0,05). As a result of quantitative real time RT-PCR analysis,the level of ADAMTS8 mRNA increased 3.5 fold in hip degenerative arthritis group when compared withfemoral neck fractures group. ADAMTS1, ADAMTS4 and ADAMTS5 expression levels in hip degenerativearthritis group were decreased 2.5, 2 and 2.5 fold, respectively. ADAMTS9, 15 were found to be similarbetween two groups.Concluson: As a result of this study on hip osteoarthritis, the ADAMTS8 levels was found to be significantly higher in the end stage of hip osteoarthritis. Unlike similar studies on knee osteoarthritis,ADAMTS1,4,5 levels were found to be lower.

Anahtar Kelime:

Konular: Ortopedi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Bibliyografik

Steinberg J, Zeggini E. Functional genomics in osteoarthritis: past, present, and future. J Orthop Res. 2016;34:1105e1110.
Atik OS, Erdogan D, Seymen CM, et al. Is there crosstalk between subc hondral bone, cartilage, and meniscus in the pathogenesis of osteoarthritis? Eklem Hastalık Cerrahisi. 2016;27:62e67.
Apte SS. A disintegrin-like and metalloprotease (reprolysin-type) with thrombospondin type 1 motif (ADAMTS) superfamily: functions and mechanisms. J Biol Chem. 2009;284:31493e314977.
Glasson SS, Askew R, Sheppard B, et al. Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature. 2005;434: 644e648.
Stanton H, Rogerson FM, East CJ, et al. ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro. Nature. 2005;434:648e652.
Rogerson FM, Chung YM, Deutscher ME, et al. Cytokine-induced increases in ADAMTS-4 mRNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice. Arthritis Rheum. 2010;62:3365e3373.
Fosang AJ, Rogerson FM. Identifying the human aggrecanase. Osteoarthritis Cartilage. 2010;18:1109e1116.
Kellgren JH, Lawrence JS. Radiological assessment of osteo-arthrosis. Ann Rheum Dis. 1957;16:494e502.
Kevorkian L, Young DA, Darrah C, et al. Expression profiling of metalloproteinases and their inhibitors in cartilage. Arthritis Rheum. 2004;50:131e141.
Atik OS. The role of metabolomics in osteoarthritis for early diagnosis, monitoring prognosis and treatment. Eklem Hastalık Cerrahisi. 2015;26:1.
Verma P, Dalal K. ADAMTS-4 and ADAMTS-5: key enzymes in osteoarthritis. J Cell Biochem. 2011;112:3507e3514.
van den Berg WB. Osteoarthritis year 2010 in review: pathomechanisms. Osteoarthritis Cartilage. 2011;19:338e341.
Collins-Racie LA. ADAMTS-8 exhibits aggrecanase activity and is expressed in human articular cartilage. Matrix Biol. 2014;23:219e230.
Somerville RPT. Characterization of ADAMTS-9 and ADAMTS-20 as a distinct. ADAMTS subfamily related to Caenorhabditis elegans GON-1. JBiol Chem. 2003;278:9503e9513.
Rodrıquez-Manzaneque JC. ADAMTS1 cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors. Biochem Biophys Res Commun. 2002;293:501e508.
Malfait AM, Liu RQ, Ijiri K. Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage. J Biol Chem. 2002;277: 22201e22208.
Ilic MZ, East CJ, Rogerson FM. Distinguishing aggrecan loss from aggrecan proteolysis in ADAMTS-4 and ADAMTS-5 single and double deficient mice. J Biol Chem. 2007;282:37420e37428.
Bau B, Gebhard PM, Haag J, et al. Relative messenger RNA expression profiling of collagenases and aggrecanases in human articular chondrocytes in vivo and in vitro. Arthritis Rheum. 2002;46:2648e2657.
Song RH, Tortorella MD, Malfait AM, et al. Aggrecan degradation in human articular cartilage explants is mediated by both ADAMTS-4 and ADAMTS-5. Arthritis Rheum. 2007;56:575e585.
Naito S, Shiomi T, Okada A. Expression of ADAMTS4 (aggrecanase-1) in human osteoarthritic cartilage. Pathol Int. 2007;57:703e711.
Zhang E, Yan X, Zhang M, et al. Aggrecanases in the human synovial fluid at different stages of osteoarthritis. Clin Rheumatol. 2010;32:797e803.
Demircan K, Gürlü G, Güven KN, et al. The potential link of COMP-ADAMTS and aggrecanase-ADAMTS genes with osteoarthrit. FASEB J. 2016;30(Supp):645e651.
Vankemmelbeke MN, Holen I, Wilson AG, et al. Expression and activity of ADAMTS-5 in synovium. Eur J Biochem. 2001;268:1259e1268.
Larkin J, Lohr TA, Elefante L, et al. Translational development of an ADAMTS-5 antibody for osteoarthritis disease modification. Osteoarthritis Cartilage. 2015 Aug;23(8):1254e1266.

APA	AYANOĞLU T, Atalar H, Esen E, Ataoğlu B, Turanlı S, DEMİRCAN K (2019). The role of ADAMTS genes in the end stage of hip osteoarthritis. , 140 - 144.
Chicago	AYANOĞLU Tacettin,Atalar Hakan,Esen Erdinç,Ataoğlu Baybars,Turanlı Sacit,DEMİRCAN Kadir The role of ADAMTS genes in the end stage of hip osteoarthritis. (2019): 140 - 144.
MLA	AYANOĞLU Tacettin,Atalar Hakan,Esen Erdinç,Ataoğlu Baybars,Turanlı Sacit,DEMİRCAN Kadir The role of ADAMTS genes in the end stage of hip osteoarthritis. , 2019, ss.140 - 144.
AMA	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K The role of ADAMTS genes in the end stage of hip osteoarthritis. . 2019; 140 - 144.
Vancouver	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K The role of ADAMTS genes in the end stage of hip osteoarthritis. . 2019; 140 - 144.
IEEE	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K "The role of ADAMTS genes in the end stage of hip osteoarthritis." , ss.140 - 144, 2019.
ISNAD	AYANOĞLU, Tacettin vd. "The role of ADAMTS genes in the end stage of hip osteoarthritis". (2019), 140-144.

APA	AYANOĞLU T, Atalar H, Esen E, Ataoğlu B, Turanlı S, DEMİRCAN K (2019). The role of ADAMTS genes in the end stage of hip osteoarthritis. Acta Orthopaedica et Traumatologica Turcica, 53(2), 140 - 144.
Chicago	AYANOĞLU Tacettin,Atalar Hakan,Esen Erdinç,Ataoğlu Baybars,Turanlı Sacit,DEMİRCAN Kadir The role of ADAMTS genes in the end stage of hip osteoarthritis. Acta Orthopaedica et Traumatologica Turcica 53, no.2 (2019): 140 - 144.
MLA	AYANOĞLU Tacettin,Atalar Hakan,Esen Erdinç,Ataoğlu Baybars,Turanlı Sacit,DEMİRCAN Kadir The role of ADAMTS genes in the end stage of hip osteoarthritis. Acta Orthopaedica et Traumatologica Turcica, vol.53, no.2, 2019, ss.140 - 144.
AMA	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K The role of ADAMTS genes in the end stage of hip osteoarthritis. Acta Orthopaedica et Traumatologica Turcica. 2019; 53(2): 140 - 144.
Vancouver	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K The role of ADAMTS genes in the end stage of hip osteoarthritis. Acta Orthopaedica et Traumatologica Turcica. 2019; 53(2): 140 - 144.
IEEE	AYANOĞLU T,Atalar H,Esen E,Ataoğlu B,Turanlı S,DEMİRCAN K "The role of ADAMTS genes in the end stage of hip osteoarthritis." Acta Orthopaedica et Traumatologica Turcica, 53, ss.140 - 144, 2019.
ISNAD	AYANOĞLU, Tacettin vd. "The role of ADAMTS genes in the end stage of hip osteoarthritis". Acta Orthopaedica et Traumatologica Turcica 53/2 (2019), 140-144.