Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey

UMUR, Sinasi; TÜMAY GÜRLER, Ali; PEKMEZCİ, Didem; HÖKELEK, Murat; BÖLÜKBAŞ, Cenk Soner; PEKMEZCI, Gokmen Zafer

doi:10.1501/Vetfak_0000002717

Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey

Cenk Soner BÖLÜKBAŞ, (Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Parasitology, Samsun, Turkey)

Gokmen Zafer PEKMEZCI, (Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Preclinical Science, Samsun, Turkey)

Ali TÜMAY GÜRLER, (Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Parasitology, Samsun, Turkey)

Didem PEKMEZCİ, (Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Internal Medicine, Samsun, Turkey)

Murat HÖKELEK, (İstanbul University, Cerrahpaşa Faculty of Medicine, Department of Microbiology, İstanbul, Turkey)

Sinasi UMUR (Ondokuz Mayıs University, Faculty of Veterinary Medicine, Department of Parasitology, Samsun, Turkey)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

4 0

Yıl: 2016 Cilt: 63 Sayı: 2 Sayfa Aralığı: 111 - 114 Metin Dili: İngilizce DOI: 10.1501/Vetfak_0000002717 İndeks Tarihi: 10-12-2019

Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey

Öz:

The World Health Organization considers leishmaniasis to be one of the most important zoonotic diseases with approximately 350 million people in 98 countries at risk of contracting the disease. The leishmaniasis also remains a severe public health problem in Turkey. It is important to evaluate the prevalence of Canine leishmaniasis (CanL) for designing control policies. In the present study, we report for the first time the occurrence of Leishmania infection in dogs living in the Middle Black Sea Region of Turkey. For the diagnosis of the infection parasitological (direct microscopic examination of lymph nodes), serological (ELISA) and molecular (kDNA-PCR) methods were performed using blood samples obtained from 240 dogs. Sand flies were also collected using CDC miniature light traps from the same locations for testing Leishmania presence by kDNA-PCR. Only one (0.41%) dog out of 240 was found to be positive by both tests. Amastigotes of Leishmania spp. were detected in the same animal lymph node aspirate sample. A total of 18 sand flies were collected and no Leishmania was detected Phlebotomus spp. sample pool. Our results showed that CanL could have public health implications and future screening studies are needed in this region of Turkey.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Aslantaş Ö, Özdemir V, Kılıç S, et al (2005): Seroepidemiology of leptospirosis, toxoplasmosis, and leishmaniosis among dogs in Ankara, Turkey. Vet Parasitol, 129, 187-191.
2. Atasoy A, Paşa S, Özensoy Toz S, et al (2010): Seroprevalence of canine visceral leishmaniasis around the Aegean cost of Turkey. Kafkas Univ Vet Fak Derg, 16, 1-6.
3. Baneth G, Koutinas AF, Solano-Gallego L, et al (2008): Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part one. Trends Parasitol, 24, 324-330.
4. Ertabaklar H, Özensoy Toz S, Taylan Özkan A, et al (2005): Serological and entomological survey in a zoonotic visceral leishmaniasis focus of North Central Anatolia, Turkey: Corum province. Acta Trop, 93, 239-246.
5. Gomes AHS, Ferreira IMR, Lima MLSR, et al (2007): PCR identification of Leishmania in diagnosis and control of canine leishmaniasis. Vet Parasitol, 144, 234-241.
6. Miró G, Cardoso L, Pennisi MG, et al (2008): Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part two. Trends Parasitol, 24, 371-377.
7. Motazedian H, Karamian M, Noyes HA, et al (2002): DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasit, 96, 31-34.
8. Ok ÜZ, Balcıoğlu İC, Özkan AT, et al (2002): Leishmaniasis in Turkey. Acta Tropica, 84, 43-48.
9. Özbel Y (2013): The infections transmitted by Sand flies in Turkey. Ankara Univ Vet Fak Derg, 60, 225-228.
10. Özbel Y, Oskam L, Özensoy S, et al (2000): A survey on canine leishmaniasis in western Turkey by parasite, DNA and antibody detection assays. Acta Tropica, 74, 1-6.
11. Özensoy Toz S, Ertabaklar H, Özbel Y, et al (2005): Seroprevalance of canine visceral leishmaniasis in Kusadasi, Turkey. Turkish J Vet Anim Sci, 29, 23-26.
12. Özensoy Toz S, Özbel Y, Ertabaklar H, et al (2005): Comparisons of clinical findings and serological data in the diagnosis of canine leishmaniosis. Turk J Vet Anim Sci, 29, 269-273.
13. Özensoy Toz S, Özbel Y, Turgay N, et al (1998): Serodiagnosis and epidemiology of visceral leishmaniasis in Turkey. Am J Trop Med Hyg, 59, 363-369.
14. Özensoy Toz S, Şakru N, Ertabaklar H, et al (2009): Serological and entomological survey of zoonotic visceral leishmaniasis in Denizli province, Aegean Region, Turkey. New Microbiologica, 32, 93-100.
15. Özerdem D, Eroğlu F, Genç A, et al (2009): Comparison of microscopic examination, rK39, and PCR for visceral leishmaniasis diagnosis in Turkey. Parasitol Res, 106, 197-200.
16. Reale S, Maxia L, Vitale F, et al (1999): Detection of Leishmania infantum in dogs by PCR with lymph node aspirates and blood. J Clin Microbiol, 37, 2931-2935.
17. Rodgers MR, Popper SJ, Wirth DF (1990): Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol, 71, 267-275.
18. Schallig HDFH, Oskam L (2002): Molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification. Trop Med Int Health, 7, 641-651.
19. Totan M, Hökelek M, Çetinkaya MC (2003): Five pneumonia cases associated with visceral leishmaniasis in Turkey. Intern Pediatr, 18, 185-187.

APA	BÖLÜKBAŞ C, PEKMEZCI G, TÜMAY GÜRLER A, PEKMEZCİ D, HÖKELEK M, UMUR S (2016). Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. , 111 - 114. 10.1501/Vetfak_0000002717
Chicago	BÖLÜKBAŞ Cenk Soner,PEKMEZCI Gokmen Zafer,TÜMAY GÜRLER Ali,PEKMEZCİ Didem,HÖKELEK Murat,UMUR Sinasi Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. (2016): 111 - 114. 10.1501/Vetfak_0000002717
MLA	BÖLÜKBAŞ Cenk Soner,PEKMEZCI Gokmen Zafer,TÜMAY GÜRLER Ali,PEKMEZCİ Didem,HÖKELEK Murat,UMUR Sinasi Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. , 2016, ss.111 - 114. 10.1501/Vetfak_0000002717
AMA	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. . 2016; 111 - 114. 10.1501/Vetfak_0000002717
Vancouver	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. . 2016; 111 - 114. 10.1501/Vetfak_0000002717
IEEE	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S "Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey." , ss.111 - 114, 2016. 10.1501/Vetfak_0000002717
ISNAD	BÖLÜKBAŞ, Cenk Soner vd. "Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey". (2016), 111-114. https://doi.org/10.1501/Vetfak_0000002717

APA	BÖLÜKBAŞ C, PEKMEZCI G, TÜMAY GÜRLER A, PEKMEZCİ D, HÖKELEK M, UMUR S (2016). Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(2), 111 - 114. 10.1501/Vetfak_0000002717
Chicago	BÖLÜKBAŞ Cenk Soner,PEKMEZCI Gokmen Zafer,TÜMAY GÜRLER Ali,PEKMEZCİ Didem,HÖKELEK Murat,UMUR Sinasi Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi 63, no.2 (2016): 111 - 114. 10.1501/Vetfak_0000002717
MLA	BÖLÜKBAŞ Cenk Soner,PEKMEZCI Gokmen Zafer,TÜMAY GÜRLER Ali,PEKMEZCİ Didem,HÖKELEK Murat,UMUR Sinasi Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.63, no.2, 2016, ss.111 - 114. 10.1501/Vetfak_0000002717
AMA	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2016; 63(2): 111 - 114. 10.1501/Vetfak_0000002717
Vancouver	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2016; 63(2): 111 - 114. 10.1501/Vetfak_0000002717
IEEE	BÖLÜKBAŞ C,PEKMEZCI G,TÜMAY GÜRLER A,PEKMEZCİ D,HÖKELEK M,UMUR S "Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63, ss.111 - 114, 2016. 10.1501/Vetfak_0000002717
ISNAD	BÖLÜKBAŞ, Cenk Soner vd. "Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey". Ankara Üniversitesi Veteriner Fakültesi Dergisi 63/2 (2016), 111-114. https://doi.org/10.1501/Vetfak_0000002717