Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method
Yıl: 2019 Cilt: 36 Sayı: 2 Sayfa Aralığı: 96 - 105 Metin Dili: İngilizce DOI: 10.4274/balkanmedj.galenos.2018.2017.1244 İndeks Tarihi: 17-01-2020
Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method
Öz: Background: Flavonoids are natural compounds with antioxidant, anticarcinogenic, and anti-inflammatory effects.Aims: To determine the cytotoxic effects of flavonoids and drug resistance related to P-gp on K562 human chronic myeloid leukemia cells. We also aimed to evaluate the therapeutic potential of imatinib and flavonoid combinations.Study Design: Cell culture study.Methods: In this study, K562 cells were treated with apigenin, luteolin, 5-desmethyl sinensetin and the anticancer drug imatinib mesylate. The effect of flavonoids on K562 cell proliferation was detected using the 3-(4,5-dimethylthiazolyl)2,5‑diphenyl‑tetrazolium bromide assay. Concentrations of apigenin, luteolin, and 5-desmethyl sinensetin ranging from 25 to 200 μM and of imatinib from 5 to 50 μM administered for 72 h were studied. Apoptosis/necrosis and P-gp activity were measured using flow cytometry. The combined effects of different concentrations of flavonoids with imatinib were evaluated according to combination index values calculated using CompuSyn software.Results: In our study, the IC50 values for apigenin, luteolin, and 5-desmethyl sinensetin were found to be 140 μM, 100 μM, and >200 μM, respectively. Luteolin (100 μM) had the highest cytotoxic activity of these flavonoids. These results were statistically significant (p<0.05). Among the flavonoids studied, the combination of luteolin and imatinib was the most effective and is therefore recommended for its cytotoxic activity in the K562 cell line. After 72 h of incubation at their respective IC50 concentrations, all flavonoids were associated with an apoptosis rate of approximately 50%. P-glycoprotein activity was increased in all groups. Combination treatment may provide better outcomes in terms of cytotoxicity and thus reduce the dosages of imatinib used.Conclusion: The combination of some flavonoids and imatinib mesylate may increase the cytotoxic effect; However, the antagonistic effect should be considered in combined use on k562 cells.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | DANIŞMAN KALINDEMİRTAŞ F, BİRMAN H, ÇANDÖKEN E, GAZİOĞLU S, Melikoğlu G, erdem kuruca s (2019). Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. , 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| Chicago | DANIŞMAN KALINDEMİRTAŞ Ferdane,BİRMAN Hüsniye,ÇANDÖKEN Eda,GAZİOĞLU Sema Bilgiç,Melikoğlu Gülay,erdem kuruca serap Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. (2019): 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| MLA | DANIŞMAN KALINDEMİRTAŞ Ferdane,BİRMAN Hüsniye,ÇANDÖKEN Eda,GAZİOĞLU Sema Bilgiç,Melikoğlu Gülay,erdem kuruca serap Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. , 2019, ss.96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| AMA | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. . 2019; 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| Vancouver | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. . 2019; 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| IEEE | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s "Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method." , ss.96 - 105, 2019. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| ISNAD | DANIŞMAN KALINDEMİRTAŞ, Ferdane vd. "Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method". (2019), 96-105. https://doi.org/10.4274/balkanmedj.galenos.2018.2017.1244 |
| APA | DANIŞMAN KALINDEMİRTAŞ F, BİRMAN H, ÇANDÖKEN E, GAZİOĞLU S, Melikoğlu G, erdem kuruca s (2019). Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal, 36(2), 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| Chicago | DANIŞMAN KALINDEMİRTAŞ Ferdane,BİRMAN Hüsniye,ÇANDÖKEN Eda,GAZİOĞLU Sema Bilgiç,Melikoğlu Gülay,erdem kuruca serap Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal 36, no.2 (2019): 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| MLA | DANIŞMAN KALINDEMİRTAŞ Ferdane,BİRMAN Hüsniye,ÇANDÖKEN Eda,GAZİOĞLU Sema Bilgiç,Melikoğlu Gülay,erdem kuruca serap Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal, vol.36, no.2, 2019, ss.96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| AMA | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal. 2019; 36(2): 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| Vancouver | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method. Balkan Medical Journal. 2019; 36(2): 96 - 105. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| IEEE | DANIŞMAN KALINDEMİRTAŞ F,BİRMAN H,ÇANDÖKEN E,GAZİOĞLU S,Melikoğlu G,erdem kuruca s "Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method." Balkan Medical Journal, 36, ss.96 - 105, 2019. 10.4274/balkanmedj.galenos.2018.2017.1244 |
| ISNAD | DANIŞMAN KALINDEMİRTAŞ, Ferdane vd. "Cytotoxic Effects of Some Flavonoids and Imatinib on the K562 Chronic Myeloid Leukemia Cell Line: Data Analysis Using the Combination Index Method". Balkan Medical Journal 36/2 (2019), 96-105. https://doi.org/10.4274/balkanmedj.galenos.2018.2017.1244 |
