It appears that patient safety is a concept that is evaluated together with some new concepts such as the right to health and patient-centered health care. Patient safety can be expressed as the prevention of errors made due to health services, and reduction or elimination of patient damages by determining the causes of errors arising from the delivery of health care services. The minimization of medical errors that pose a threat to patient safety and may lead to the loss of labor, resource and life can be achieved by knowing the sources of error. Medication errors, hospital infections, surgical operation errors, falls and pressure ulcers are considered as the medical errors that threaten patient safety at the highest level. In this study, it was aimed to investigate whether the patient safety attitudes of nurses working in Süleyman Demirel University Research and Application Hospital had an effect on patient safety culture. For this purpose, it was planned to test the hypotheses of "there is a relationship between patient safety attitudes of nurses and their opinions on patient safety culture" and "patient safety attitudes of nurses have an effect on patient safety culture". The study was carried out on the nurses working in Süleyman Demirel University Research and Application Hospital between January and February of 2018. 341 nurses working in Süleyman Demirel University Research and Application Hospital constituted the population of the study. 200 nurses agreed to participate by giving consent and approval. The "Patient Safety Attitude Scale", "Patient Safety Culture Scale" and the questionnaire form including questions related to demographic and professional knowledge were used as data collection tools. Correlation analysis was performed to determine the relationship between the patient safety attitudes of nurses and their sub-dimensions and patient safety culture and its sub-dimensions, and regression analysis was performed to determine the effects between the variables. As a result of analyses performed, it was determined that there was a relationship between the subdimensions of patient safety attitudes of nurses and the sub-dimensions of their opinions on patient safety culture and that patient safety attitudes of nurses affected patient safety culture.