Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode
Yıl: 2019 Cilt: 43 Sayı: 4 Sayfa Aralığı: 1063 - 1074 Metin Dili: İngilizce DOI: 10.3906/kim-1901-13 İndeks Tarihi: 08-05-2020
Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode
Öz: Biosensing of urea by a biosensor as a direct detection method at ambient temperature and pressureinstead of chromatography leads to a significant reduction in processing costs. Amperometric biosensors based onurease immobilization on macroporous polypyrrole (MPPy) and pyrrole on the surface of a Pt electrode were developed.Applying cyclic voltammetry (CV), we demonstrated the synthesis of MPPy using monodispersed polystyrene spheres(460 nm) as a template. CV and chronoamperometric studies were conducted to evaluate the electrochemical currentof the modified electrodes. For the electrode with polypyrrole (PPy), the biosensor response was linear in the range of1.67–8.32 mM (R2 = 0.99). Sensitivity, detection limit, and response time of this biosensor were 0.0035 mA mM −1,2.57 mM, and ∼7 s, respectively. For the electrode with MPPy, the linear range was 0.5–10.82 mM (R2 = 0.99). Forthis biosensor, sensitivity, detection limit (S/N = 3), and response time were 0.0432 mA mM −1, 0.208 mM, and ∼5 s,respectively. The modified biosensor with MPPy showed high stability and desirable selectivity for urea.
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| APA | HOSSEINIAN M, NAJAFPOUR G, RAHIMPOUR A (2019). Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. , 1063 - 1074. 10.3906/kim-1901-13 |
| Chicago | HOSSEINIAN Maliheh,NAJAFPOUR Ghasem,RAHIMPOUR Ahmad Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. (2019): 1063 - 1074. 10.3906/kim-1901-13 |
| MLA | HOSSEINIAN Maliheh,NAJAFPOUR Ghasem,RAHIMPOUR Ahmad Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. , 2019, ss.1063 - 1074. 10.3906/kim-1901-13 |
| AMA | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. . 2019; 1063 - 1074. 10.3906/kim-1901-13 |
| Vancouver | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. . 2019; 1063 - 1074. 10.3906/kim-1901-13 |
| IEEE | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A "Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode." , ss.1063 - 1074, 2019. 10.3906/kim-1901-13 |
| ISNAD | HOSSEINIAN, Maliheh vd. "Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode". (2019), 1063-1074. https://doi.org/10.3906/kim-1901-13 |
| APA | HOSSEINIAN M, NAJAFPOUR G, RAHIMPOUR A (2019). Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. Turkish Journal of Chemistry, 43(4), 1063 - 1074. 10.3906/kim-1901-13 |
| Chicago | HOSSEINIAN Maliheh,NAJAFPOUR Ghasem,RAHIMPOUR Ahmad Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. Turkish Journal of Chemistry 43, no.4 (2019): 1063 - 1074. 10.3906/kim-1901-13 |
| MLA | HOSSEINIAN Maliheh,NAJAFPOUR Ghasem,RAHIMPOUR Ahmad Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. Turkish Journal of Chemistry, vol.43, no.4, 2019, ss.1063 - 1074. 10.3906/kim-1901-13 |
| AMA | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. Turkish Journal of Chemistry. 2019; 43(4): 1063 - 1074. 10.3906/kim-1901-13 |
| Vancouver | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode. Turkish Journal of Chemistry. 2019; 43(4): 1063 - 1074. 10.3906/kim-1901-13 |
| IEEE | HOSSEINIAN M,NAJAFPOUR G,RAHIMPOUR A "Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode." Turkish Journal of Chemistry, 43, ss.1063 - 1074, 2019. 10.3906/kim-1901-13 |
| ISNAD | HOSSEINIAN, Maliheh vd. "Amperometric urea biosensor based on immobilized urease on polypyrrole and macroporous polypyrrole modified Pt electrode". Turkish Journal of Chemistry 43/4 (2019), 1063-1074. https://doi.org/10.3906/kim-1901-13 |
