Objectives: Single-incision laparoscopic procedures have been gaining popularity in recent years due to their minimal incisionalmorbidity and improved cosmetic outcomes. Herein, we will compare the feasibility and outcomes of single-incision and conventional multiport laparoscopic distal pancreatectomy.Methods: This study involves consecutive patients who underwent either single-incision or conventional multiport laparoscopicdistal pancreatectomy from March 2007 to February 2014. The patients were divided into two groups, based on the surgical approach: single-incision laparoscopic surgery (Group 1) and conventional multiport laparoscopic surgery (Group 2). The parametersevaluated included estimated blood loss, operative time, postoperative pain, duration of hospital stay, complications, conversion,and final pathology.Results: There were a total of 20 patients, 10 in each study group. Baseline characteristics were similar, and there was no mortalityin either of the groups. The low-flow pancreatic fistula was the most commonly seen morbidity, but it was ceased spontaneouslyin each group by a ratio of 30%. The operative time was significantly shorter in Group 2, with a mean of 116 minutes versus a meanof 180 minutes for Group 1 (p<0.001). One of the procedures in Group 2 was converted to open surgery due to peroperative bleeding. The median follow-up periods of the patients were 22 months and 56 months. The spleen was preserved in only 1 patient fromGroup 1, whereas in Group 2, the spleen preservation was achieved in 5 patients.Conclusion: The single-incision laparoscopic technique is a safe and effective alternative to standard laparoscopic distal pancreatectomy