Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines
Yıl: 2018 Cilt: 15 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: İngilizce DOI: 10.4274/tjps.80764 İndeks Tarihi: 18-08-2020
Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines
Öz: Objectives: Vanadium compounds have various pharmacologic effects and all available evidence reveals that the effects of vanadium compoundsdepend on many factors, mainly on the type of cells and dose. The proapoptotic or antiapoptotic effect of vanadium compounds depends stronglyon the cell type.Materials and Methods: In this study, the effects of vanadium pentoxide (V2O5) were investigated using several tumor cell lines: a colorectal cancercell line (Colo-205), a human breast adenocarcinoma cell line (MCF-7), and a normal human fibroblast cell line. Five different concentrations of V2O5between 25-200 µM were applied on the cells and xCELLigence real-time cell analysis was conducted to evaluate the impedance alterations. Thisstudy is the first to show V2O5’s effects on Colo-205 and MCF-7 and human fibroblast cell lines in a real-time manner.Results: In the Colo-205 cell line, cell index (CI) alterations decreased slightly at 25 µM and 50 µM, and increased at 100 µM, 150 µM and 200 µMconcentrations. In the MCF-7 cell line, CI alterations increased at all concentrations compared with the untreated control. However, in the healthyfibroblast cell line, the CI alterations decreased at all concentrations compared with the untreated control, which limits the use of V2O5 for itscytotoxic effect in vivo.Conclusion: The combination of conventional anticancer drugs can be used to increase the effectiveness and reduce the adverse effects of thesedrugs considering stages of cancer and cancer type. Our results suggest that V2O5 has disparate effects on several cancer cells at differentconcentrations.
Anahtar Kelime: Vanadyum Pentoksidin Çeşitli Kanser Hücre Hatlarındaki Etkilerinin Empedans Değişiklikleri ile Gerçek Zamanlı Analizi
Öz: Amaç: Vanadyum bileşiklerinin çeşitli farmakolojik etkileri vardır ve tüm kanıtlar, vanadyum bileşiklerinin etkilerinin, başta doz ve hücre tipi olmak üzere birçok faktöre bağlı olduğunu ortaya koymaktadır. Vanadyum bileşiklerinin proapoptotik veya antiapoptotik etkisi esas olarak hücre türüne bağlıdır. Gereç ve Yöntemler: Bu çalışmada, çeşitli kanser hücre hatlarında; kolorektal kanser hücre hattı (Colo-205), insan meme adenokarsinoma hücre hattı (MCF-7) ve normal insan fibroblast hücre hattı kullanılarak vanadyum pentoksidin (V2 O5 ) sitotoksik aktivitesi araştırıldı. Sitotoksik etkilerini incelemek için V2 O525-200 μM arasında 5 farklı konsantrasyonda hücrelere uygulandı ve empedans değişikliklerini değerlendirmek için xCELLigence gerçek zamanlı hücre analizi yapıldı. Bu çalışma, V2 O5 ’in Colo-205, MCF-7 ve insan fibroblast hücre hatlarındaki etkilerini gerçek zamanlı olarak gösteren ilk çalışmadır. Bulgular: V2 O5 , normal insan fibroblastında 12 saat sonuçlarına göre tüm konsantrasyonlarda Colo-205 ve MCF-7’den daha düşük sitotoksik etki gösterdi. Sağlıklı fibroblast hücre hattında, cell indeks (CI) kontrol ile karşılaştırıldığında tüm konsantrasyonlarda azaldı. Colo-205 hücre hattında CI, 25 µM ve 50 µM’de azaldı; 100 µM, 150 µM ve 200 µM konsantrasyonlarında arttı. MCF-7 hücre hattında, CI kontrole kıyasla tüm konsantrasyonlarda arttı. Sonuç: Konvansiyonel anti-kanser ilaçlar ile kombinasyonu, kanser türü ve evreleri dikkate alınarak bu ilaçların yan etkilerini azaltmak ve etkinliği arttırmak için kullanılabilir. Bulgularımız; V2 O5 ’in farklı konsantrasyonlarda çeşitli kanser hücreleri üzerinde farklı etkilere sahip olduğunu düşündürmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Ozturk E, Arslan A, dokumacı a, Yerer Aycan M (2018). Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. , 1 - 6. 10.4274/tjps.80764 |
| Chicago | Ozturk Ebru,Arslan Ayşe Kübra Karaboğa,dokumacı alim hüseyin,Yerer Aycan Mukerrem Betul Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. (2018): 1 - 6. 10.4274/tjps.80764 |
| MLA | Ozturk Ebru,Arslan Ayşe Kübra Karaboğa,dokumacı alim hüseyin,Yerer Aycan Mukerrem Betul Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. , 2018, ss.1 - 6. 10.4274/tjps.80764 |
| AMA | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. . 2018; 1 - 6. 10.4274/tjps.80764 |
| Vancouver | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. . 2018; 1 - 6. 10.4274/tjps.80764 |
| IEEE | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M "Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines." , ss.1 - 6, 2018. 10.4274/tjps.80764 |
| ISNAD | Ozturk, Ebru vd. "Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines". (2018), 1-6. https://doi.org/10.4274/tjps.80764 |
| APA | Ozturk E, Arslan A, dokumacı a, Yerer Aycan M (2018). Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. Turkish Journal of Pharmaceutical Sciences, 15(1), 1 - 6. 10.4274/tjps.80764 |
| Chicago | Ozturk Ebru,Arslan Ayşe Kübra Karaboğa,dokumacı alim hüseyin,Yerer Aycan Mukerrem Betul Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. Turkish Journal of Pharmaceutical Sciences 15, no.1 (2018): 1 - 6. 10.4274/tjps.80764 |
| MLA | Ozturk Ebru,Arslan Ayşe Kübra Karaboğa,dokumacı alim hüseyin,Yerer Aycan Mukerrem Betul Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. Turkish Journal of Pharmaceutical Sciences, vol.15, no.1, 2018, ss.1 - 6. 10.4274/tjps.80764 |
| AMA | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. Turkish Journal of Pharmaceutical Sciences. 2018; 15(1): 1 - 6. 10.4274/tjps.80764 |
| Vancouver | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines. Turkish Journal of Pharmaceutical Sciences. 2018; 15(1): 1 - 6. 10.4274/tjps.80764 |
| IEEE | Ozturk E,Arslan A,dokumacı a,Yerer Aycan M "Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines." Turkish Journal of Pharmaceutical Sciences, 15, ss.1 - 6, 2018. 10.4274/tjps.80764 |
| ISNAD | Ozturk, Ebru vd. "Real-time Analysis of Impedance Alterations by the Effects of Vanadium Pentoxide on Several Carcinoma Cell Lines". Turkish Journal of Pharmaceutical Sciences 15/1 (2018), 1-6. https://doi.org/10.4274/tjps.80764 |
